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Antimicrobial Agents and Chemotherapy, October 1999, p. 2520-2523, Vol. 43, No. 10
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Drug Efflux and parC Mutations Are Involved in Fluoroquinolone Resistance in Viridans Group Streptococci

María José Ferrándiz,1 Jesús Oteo,2 Belén Aracil,2 Jose Luis Gómez-Garcés,2 and Adela G. De La Campa1,*

Unidad de Genética Bacteriana (Consejo Superior de Investigaciones Científicas), Centro Nacional de Biología Fundamental, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid,1 and Servicio de Microbiología, Hospital de Móstoles, 28935 Madrid,2 Spain

Received 17 May 1999/Returned for modification 29 June 1999/Accepted 29 July 1999

Nine ciprofloxacin-resistant viridans group streptococci isolated from asymptomatic carriers were analyzed. Identification to the species level by using three different commercial systems and a PCR-based approach was inconsistent. The nucleotide sequences of fragments of the parC, parE, gyrA, and gyrB genes showed considerable intra- and interspecies variations, and these variations mainly involved silent mutations. Three isolates had changes in Ser-79 of ParC (to Phe or Tyr). Phenotypic characterization indicated that eight of the nine isolates had a putative efflux mechanism that would confer low-level resistance to ciprofloxacin.

* Corresponding author. Mailing address: Unidad de Genética Bacteriana (Consejo Superior de Investigaciones Científicas), Centro Nacional de Biología Fundamental, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain. Phone: (34) 91-509-7904. Fax: (34) 91-509-7919. E-mail: agcampa{at}isciii.es.

Antimicrobial Agents and Chemotherapy, October 1999, p. 2520-2523, Vol. 43, No. 10
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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