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Antimicrobial Agents and Chemotherapy, October 1999, p. 2538-2541, Vol. 43, No. 10
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Reconstruction of an Active Integron Recombination Site after Integration of a Gene Cassette at a Secondary Site

Heidi Segal,1 M. Victoria Francia,2 Juan M. García Lobo,2 and Gay Elisha1,3,*

Department of Medical Microbiology, University of Cape Town,1 and Groote Schuur Hospital,3 Cape Town, South Africa, and Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, 39011 Santander, Spain2

Received 21 January 1999/Returned for modification 24 May 1999/Accepted 22 July 1999

As the site of insertion of the aadB gene cassette on pRAY, from a clinical isolate of Acinetobacter, is almost identical to the preferred site on integrons, the composite 59-base element (59-BE) associated with this cassette is potentially recombinationally active. By using a conduction assay to quantitate site activity, the 59-BE was recognized by integrase with high frequency, indicating that the composite site is recombinationally active.

* Corresponding author. Mailing address: Department of Medical Microbiology, Medical School UCT, Anzio Rd., Observatory 7925, Cape Town, South Africa. Phone: (27) (21) 406 6378. Fax: (27) (21) 448 8153. E-mail: gelisha{at}medmicro.uct.ac.za.

Antimicrobial Agents and Chemotherapy, October 1999, p. 2538-2541, Vol. 43, No. 10
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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