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Antimicrobial Agents and Chemotherapy, November 1999, p. 2780-2782, Vol. 43, No. 11
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Distribution of Insertion Sequence-Like Element IS1272 and Its Position Relative to Methicillin Resistance Genes in Clinically Important Staphylococci

Nobumichi Kobayashi,1,* Shozo Urasawa,1 Nobuyuki Uehara,2 and Naoki Watanabe2

Department of Hygiene1 and Department of Laboratory Diagnosis,2 Sapporo Medical University School of Medicine, Chuo-ku, Sapporo 060-8556, Japan

Received 14 May 1999/Returned for modification 29 June 1999/Accepted 18 August 1999

The distribution of insertion sequence-like element IS1272 was analyzed for clinical isolates of Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus haemolyticus. In each of the staphylococcal species, IS1272 was detected in both methicillin-resistant (MR) and methicillin-susceptible strains of different genetic types. In MR isolates, IS1272 was generally located downstream of the truncated mecR1 gene (Delta mecR1), with an identical junction sequence occurring between Delta mecR1 and IS1272, although insertion of an additional gene sequence in the junction sequence was detected in one S. epidermidis isolate. These findings suggest that the mec element with the rearranged form of mecR1 (Delta mecR1-IS1272) has been transmitted to multiple clones of staphylococci.

* Corresponding author. Mailing address: Department of Hygiene, Sapporo Medical University School of Medicine, S-1, W-17, Chuo-ku, Sapporo 060-8556, Japan. Phone: 81-11-611-2111, ext. 2351. Fax: 81-11-612-1660. E-mail: nkobayas{at}sapmed.ac.jp.

Antimicrobial Agents and Chemotherapy, November 1999, p. 2780-2782, Vol. 43, No. 11
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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