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Antimicrobial Agents and Chemotherapy, November 1999, p. 2813-2816, Vol. 43, No. 11
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Characterization of rpoB Mutations Conferring Cross-Resistance to Rifamycins on Methicillin-Resistant Staphylococcus aureus

Thomas A. Wichelhaus,* Volker Schäfer, Volker Brade, and Boris Böddinghaus

Institute of Medical Microbiology, University Hospital of Frankfurt, Frankfurt am Main, Germany

Received 1 February 1999/Returned for modification 24 May 1999/Accepted 25 August 1999

Mutations of the rpoB gene conferring resistance to rifampin were analyzed in 40 methicillin-resistant Staphylococcus aureus isolates obtained from six countries. Interestingly, the majority of clinical isolates showed multiple mutations within rpoB. The amino acid substitution 481Hisright-arrowAsn was the most prevalent one, capable of conferring low-level resistance on its own. Cross-resistance to rifampin, rifabutin, and rifapentine was demonstrated for all mutants identified. The level of resistance to rifamycins correlated with both the mutation position and type of amino acid substitution.

* Corresponding author. Mailing address: Institute of Medical Microbiology, University Hospital of Frankfurt, Paul-Ehrlich-Str. 40, 60596 Frankfurt am Main, Germany. Phone: 49/69/6301-5019. Fax: 49/69/6301-5767. E-mail: Wichelhaus{at}em.uni-frankfurt.de.

Antimicrobial Agents and Chemotherapy, November 1999, p. 2813-2816, Vol. 43, No. 11
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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