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Antimicrobial Agents and Chemotherapy, December 1999, p. 2930-2938, Vol. 43, No. 12
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Multilocus Genotypes and DNA Fingerprints Do Not Predict Variation in Azole Resistance among Clinical Isolates of Candida albicans

Leah E. Cowen,1,* Caroline Sirjusingh,1 Richard C. Summerbell,2,3 Sharon Walmsley,4 Susan Richardson,5 Linda M. Kohn,1 and James B. Anderson1

Department of Botany, University of Toronto, Mississauga, Ontario, Canada L5L 1C61; Mycology Laboratory, Ontario Ministry of Health, Laboratories Branch, Toronto, Ontario, Canada M5W 1R52; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada M5G 1L53; Department of Medicine, Division of Infectious Disease, The Toronto Hospital, University of Toronto, Toronto, Ontario, Canada M5G 2C44; Department of Pediatric Laboratory Medicine---Microbiology, The Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X85

Received 18 May 1999/Returned for modification 17 August 1999/Accepted 1 October 1999

If variation in azole resistance is due to inherent differences in strains of Candida albicans, as a predominantly clonal organism, then correlation between multilocus genotypes and drug resistance would be expected. A sample of 81 clinical isolates from patients infected with human immunodeficiency virus in Toronto, Canada, plus 3 reference isolates were genotyped at 16 loci, distributed on all linkage groups, by means of oligonucleotide hybridizations specific for each of the alleles at each locus. These multilocus genotypes were significantly correlated with DNA fingerprints obtained with the species-specific probe 27A, indicating widespread linkage disequilibrium in the genome. There were 64 multilocus diploid genotypes and 77 DNA fingerprint types delineated in this sample. Neither the multilocus genotyping nor DNA fingerprinting alone identified all of the 81 types identified by the combination of these two methods. Multilocus genotypes were not predictive of fluconazole resistance, suggesting that resistance is gained or lost too quickly to be predicted by linkage with neutral markers.

* Corresponding author. Mailing address: Department of Botany, University of Toronto at Mississauga, 3359 Mississauga Rd. North, Mississauga, Ontario, Canada L5L 1C6. Phone: (905) 828-5338. Fax: (905) 828-3792. E-mail: lcowen{at}credit.erin.utoronto.ca

Antimicrobial Agents and Chemotherapy, December 1999, p. 2930-2938, Vol. 43, No. 12
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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