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Antimicrobial Agents and Chemotherapy, December 1999, p. 2960-2963, Vol. 43, No. 12
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Automated Thermal Cycling Is Superior to Traditional Methods for Nucleotide Sequencing of blaSHV Genes

Patricia A. Bradford*

Infectious Disease Discovery Research, Wyeth-Ayerst Research, Pearl River, New York 10965

Received 10 May 1999/Returned for modification 22 July 1999/Accepted 21 September 1999

Genes encoding SHV-1 and SHV-2 were sequenced by different methods. Nucleotide sequencing of the coding strand by standard dideoxy-chain termination methods resulted in errors in the interpretation of the nucleotide sequence and the derived amino acid sequence in two main regions which corresponded to nucleotide and amino acid changes that had been reported previously. The automated thermal cycling method was clearly superior and consistently resulted in the correct sequences for these genes.

* Mailing address: Wyeth-Ayerst Research, 401 N. Middletown Rd., Pearl River, NY 10965. Phone: (914) 732-4396. Fax: (914) 732-5671. E-mail: bradfop{at}war.wyeth.com.

Antimicrobial Agents and Chemotherapy, December 1999, p. 2960-2963, Vol. 43, No. 12
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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