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Antimicrobial Agents and Chemotherapy, December 1999, p. 2998-3000, Vol. 43, No. 12
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Activity of Gemifloxacin against Penicillin- and Ciprofloxacin-Resistant Streptococcus pneumoniae Displaying Topoisomerase- and Efflux-Mediated Resistance Mechanisms

Victoria J. Heaton,1 Colin E. Goldsmith,2 Jane E. Ambler,3 and L. Mark Fisher1,*

Molecular Genetics Group, Department of Biochemistry, St. George's Hospital Medical School, University of London, London SW17 ORE,1 Bacteriology Department, Belfast City Hospital Trust, Belfast BT9 7AB, Northern Ireland,2 and SmithKline Beecham Pharmaceuticals, Harlow, Essex CM19 5AW,3 United Kingdom

Received 1 June 1999/Returned for modification 6 July 1999/Accepted 17 September 1999

Nine penicillin-resistant Streptococcus pneumoniae clinical isolates from Northern Ireland, resistant to ciprofloxacin (MICs, 2 to 64 µg/ml) through topoisomerase- and/or reserpine-sensitive efflux mechanisms, were highly susceptible to gemifloxacin (MICs, 0.03 to 0.12 µg/ml). Two strains (requiring a ciprofloxacin MIC of 64 µg/ml) carried known quinolone resistance mutations in parC, parE, and gyrB, resulting in S79F, D435V, and E474K changes, respectively. Thus, gemifloxacin is active against clinical strains exhibiting altered topoisomerase and efflux phenotypes.

* Corresponding author. Mailing address: Molecular Genetics Group, Department of Biochemistry, St. George's Hospital Medical School, University of London, London SW17 0RE, United Kingdom. Fax: 44 181 725 2992. E-mail: lfisher{at}sghms.ac.uk.

Antimicrobial Agents and Chemotherapy, December 1999, p. 2998-3000, Vol. 43, No. 12
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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