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Antimicrobial Agents and Chemotherapy, December 1999, p. 3036-3038, Vol. 43, No. 12
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Novel Streptomycin and Spectinomycin Resistance Gene as a Gene Cassette within a Class 1 Integron Isolated from Escherichia coli

Dorthe Sandvang*

Danish Veterinary Laboratory, DK-1790 Copenhagen V, and Department of Microbiology, The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, Denmark

Received 11 June 1999/Returned for modification 20 July 1999/Accepted 29 September 1999

The aadA genes, encoding resistance to streptomycin and spectinomycin, have been found as gene cassettes in different gram-negative and gram-positive bacterial species. The present study has revealed the sequence of a new gene, aadA5, integrated as a gene cassette together with the trimethoprim resistance gene dfr7 in a class 1 integron. The integron was located on a plasmid and was identified in a pathogenic porcine Escherichia coli isolate.

* Mailing address: Danish Veterinary Laboratory, Bülowsvej 27, DK-1790 Copenhagen V, Denmark. Phone: 45 35 30 01 00. Fax: 45 35 30 01 20. E-mail: ds{at}svs.dk.

Antimicrobial Agents and Chemotherapy, December 1999, p. 3036-3038, Vol. 43, No. 12
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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