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Antimicrobial Agents and Chemotherapy, February 1999, p. 406-409, Vol. 43, No. 2
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Detection of gyrA Mutations among 335 Pseudomonas aeruginosa Strains Isolated in Japan and Their Susceptibilities to Fluoroquinolones

Takashi Takenouchi,1,* Eiko Sakagawa,1 and Mie Sugawara2

Biological Research Laboratories1 and Biomedical Research Laboratories,2 Sankyo Co., Ltd., 2-58 Hiromachi 1-chome, Shinagawa-ku, Tokyo 140-8710, Japan

Received 6 July 1998/Returned for modification 3 September 1998/Accepted 12 November 1998

gyrA point mutations in 335 clinical Pseudomonas aeruginosa isolates were examined mainly by nonisotopic single-strand conformation polymorphism analysis and direct sequencing. Seven types of missense gyrA mutations were observed in 70 of 335 strains (20.9%), and ciprofloxacin MICs were >= 3.13 µg/ml for 63 of 70 strains (90.0%). These included two double point mutations and three novel mutations (Ala-67right-arrowSer plus Asp-87right-arrowGly, Ala-84right-arrowPro, and Gln-106right-arrowLeu). Thr-83right-arrowIle mutants were predominantly observed (63 of 70 mutants) and showed high-level fluoroquinolone resistance (ciprofloxacin MIC at which 50% of isolates are inhibited, 25 µg/ml).

* Corresponding author. Mailing address: Biological Research Laboratories, Sankyo Co., Ltd., 2-58 Hiromachi 1-chome, Shinagawa-ku, Tokyo 140-8710, Japan. Phone: 81-3-3492-3131. Fax: 81-3-5436-8566. E-mail: takeno{at}shina.sankyo.co.jp.

Antimicrobial Agents and Chemotherapy, February 1999, p. 406-409, Vol. 43, No. 2
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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