Previous Article | Next Article ![]()
Antimicrobial Agents and Chemotherapy, March 1999, p. 693-696, Vol. 43, No. 3
Laboratories of
Ultrastructures,1 Bacteriology and
Medical Mycology,2 and Cellular
Biology,3 Istituto Superiore di
Sanità, 00161 Rome, Italy
Received 9 September 1998/Returned for modification 21 October
1998/Accepted 30 December 1998
Multidrug-resistant Vibrio cholerae O1 El Tor strains
isolated during the 1994 outbreak of cholera in Albania and Italy were characterized for the molecular basis of antibiotic resistance. All
strains were found to be resistant to tetracycline, streptomycin, spectinomycin, trimethoprim, sulfathiazole, and the vibriostatic compound O/129 (2,4-diamino-6,7-diisopropylteridine). Resistance genes
were self-transferable by a conjugative plasmid of about 60 MDa, with
the exception of spectinomycin resistance, which was conferred by the
aadA1 gene cassette located in the bacterial chromosome
within a class 1 integron. The resistance to trimethoprim and O/129 was
conferred by the dfrA1 gene, which was present on the
plasmid. Although the dfrA1 gene is known to be borne on an integron cassette, class 1, 2, or 3 intI genes were not
detected as part of the plasmid DNA from the strains studied.
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Antibiotic Resistance Conferred by a Conjugative
Plasmid and a Class I Integron in Vibrio cholerae O1 El Tor
Strains Isolated in Albania and Italy
*
Corresponding author. Mailing address: Laboratory of
Ultrastructures, Istituto Superiore di Sanità, Viale Regina
Elena, 299, 00161 Rome, Italy. Phone: 39 6 4990 2805. Fax: 39 6 49387112.
This article has been cited by other articles:
| Clin. Vaccine Immunol. | Clin. Microbiol. Rev. |
|---|---|
| J. Clin. Microbiol. | ALL ASM JOURNALS |