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Antimicrobial Agents and Chemotherapy, April 1999, p. 925-929, Vol. 43, No. 4
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A New Resistance Gene, linB, Conferring Resistance to Lincosamides by Nucleotidylation in Enterococcus faecium HM1025

Bülent Bozdogan,1,2 Latifa Berrezouga,1 Ming-Shang Kuo,3 David A. Yurek,3 Kathleen A. Farley,3 Brian J. Stockman,3 and Roland Leclercq1,2,*

Service de Bactériologie-Virologie, Hôpital Henri Mondor---Université Paris XII, 94000 Créteil,1 and Service de Microbiologie, Hôpital Côte de Nacre, Université de Caen, 14033 Caen,2 France, and Pharmacia and Upjohn, Kalamazoo, Michigan 490023

Received 3 August 1998/Returned for modification 15 September 1998/Accepted 6 January 1999

Resistance to lincomycin and clindamycin in the clinical isolate Enterococcus faecium HM1025 is due to a ribosomal methylase encoded by an ermAM-like gene and the plasmid-mediated inactivation of these antibiotics. We have cloned and determined the nucleotide sequence of the gene responsible for the inactivation of lincosamides, linB. This gene encodes a 267-amino-acid lincosamide nucleotidyltransferase. The enzyme catalyzes 3-(5'-adenylation) (the adenylation of the hydroxyl group in position 3 of the molecules) of lincomycin and clindamycin. Expression of linB was observed in both Escherichia coli and Staphylococcus aureus. The deduced amino acid sequence of the enzyme did not display any significant homology with staphylococcal nucleotidyltransferases encoded by linA and linA' genes. Sequences homologous to linB were found in 14 other clinical isolates of E. faecium, indicating the spread of the resistance trait in this species.

* Corresponding author. Mailing address: CHU de Caen, Service de Microbiologie, Avenue Côte de Nacre, 14033 Caen cedex, France. Phone: (33) 2 31 06 45 72. Fax: (33) 2 31 06 45 73. E-mail: leclercq-r{at}chu-caen.fr.

Antimicrobial Agents and Chemotherapy, April 1999, p. 925-929, Vol. 43, No. 4
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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