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Antimicrobial Agents and Chemotherapy, April 1999, p. 969-971, Vol. 43, No. 4
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Sequences of the Genes for the TEM-20, TEM-21, TEM-22, and TEM-29 Extended-Spectrum beta -Lactamases

Guillaume Arlet,1,* Sylvie Goussard,2 Patrice Courvalin,2 and Alain Philippon1,dagger

Service de Microbiologie, Hôpital Saint-Louis, Université Paris VII, 75475 Paris Cedex 10,1 and Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15,2 France

Received 22 July 1998/Returned for modification 3 November 1998/Accepted 3 January 1999

The sequences of the blaTEM genes encoding TEM-20, TEM-21, TEM-22, and TEM-29 extended-spectrum beta -lactamases were determined. Analysis of the deduced amino acid sequences indicated that TEM-20 and TEM-29 were derived from TEM-1 and that TEM-21 and TEM-22 were derived from TEM-2. The substitutions involved were Ser-238 and Thr-182 for TEM-20; His-164 for TEM-29; Lys-104, Arg-153, and Ser-238 for TEM-21; and Lys-104, Gly-237, and Ser-238 for TEM-22. The promoter region of the blaTEM-22 gene was identical to that of blaTEM-3. High-level production of TEM-20 could result from a 135-bp deletion which combined the -35 region of the Pa promoter with the -10 region of the P3 promoter and a Gright-arrowT transition in the latter motif.

* Corresponding author. Mailing address: Service de Microbiologie, Hôpital Saint-Louis, 1, Av. Claude Vellefaux, 75475, Paris Cedex 10, France. Phone: (33) (1) 42 49 93 48. Fax: (33) (1) 42 49 92 00. E-mail: guillaume.arlet{at}tnn.ap-hop-paris.fr.

dagger Present address: Service de Microbiologie, Hôpital Cochin, 75014 Paris, France.

Antimicrobial Agents and Chemotherapy, April 1999, p. 969-971, Vol. 43, No. 4
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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