This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lina, G.
Right arrow Articles by Etienne, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lina, G.
Right arrow Articles by Etienne, J.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, May 1999, p. 1062-1066, Vol. 43, No. 5
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Distribution of Genes Encoding Resistance to Macrolides, Lincosamides, and Streptogramins among Staphylococci

Gerard Lina,1,* Alain Quaglia,1 Marie-Elisabeth Reverdy,1 Roland Leclercq,2 François Vandenesch,1 and Jerome Etienne1

Faculté de Médecine, Laboratoire de Bactériologie, Centre National de Référence des Toxémies à Staphylocoques, EA 1655, 69372 Lyon Cedex 08,1 and Service de Microbiologie, CHU Côte de Nacre, 14033 Caen,2 France

Received 7 October 1998/Returned for modification 15 January 1999/Accepted 11 February 1999

The relative frequency of 10 determinants of resistance to macrolides, lincosamides, and streptogramins was investigated by PCR in a series of 294 macrolide-, lincosamide-, and/or streptogramin-resistant clinical isolates of Staphylococcus aureus and coagulase-negative staphylococci isolated in 1995 from 32 French hospitals. Resistance was mainly due to the presence of ermA or ermC genes, which were detected in 259 strains (88%), in particular those resistant to methicillin (78% of the strains). Macrolide resistance due to msrA was more prevalent in coagulase-negative staphylococci (14.6%) than in S. aureus (2.1%). Genes related to linA/linA' and conferring resistance to lincomycin were detected in one strain of S. aureus and seven strains of coagulase-negative staphylococci. Resistance to pristinamycin and quinupristin-dalfopristin was phenotypically detected in 10 strains of S. aureus and in three strains of coagulase-negative staphylococci; it was always associated with resistance to type A streptogramins encoded by vat or vatB genes and occurred in association with erm genes. The vga gene conferring decreased susceptibility to type A streptogramins was present alone in three strains of coagulase-negative staphylococci and in combination with erm genes in 10 strains of coagulase-negative staphylococci. A combination of vga-vgb-vat and ermA genes was found in a single strain of S. epidermidis.


* Corresponding author. Mailing address: Faculté de Médecine, Laboratoire de Bactériologie, Centre National de Référence des Toxémies à Staphylocoques, Rue Guillaume Paradin, 69372 Lyon Cedex 08, France. Phone: 33-478-77-86-57. Fax: 33-478-77-86-58. E-mail: geralina{at}univ-lyon1.fr.


Antimicrobial Agents and Chemotherapy, May 1999, p. 1062-1066, Vol. 43, No. 5
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Perez-Vazquez, M., Vindel, A., Marcos, C., Oteo, J., Cuevas, O., Trincado, P., Bautista, V., Grundmann, H., Campos, J., on behalf of the EARSS Spain spa-typing Group, (2009). Spread of invasive Spanish Staphylococcus aureus spa-type t067 associated with a high prevalence of the aminoglycoside-modifying enzyme gene ant(4')-Ia and the efflux pump genes msrA/msrB. J Antimicrob Chemother 63: 21-31 [Abstract] [Full Text]  
  • Besier, S., Zander, J., Kahl, B. C., Kraiczy, P., Brade, V., Wichelhaus, T. A. (2008). The Thymidine-Dependent Small-Colony-Variant Phenotype Is Associated with Hypermutability and Antibiotic Resistance in Clinical Staphylococcus aureus Isolates. Antimicrob. Agents Chemother. 52: 2183-2189 [Abstract] [Full Text]  
  • Mendes, R. E., Deshpande, L. M., Castanheira, M., DiPersio, J., Saubolle, M. A., Jones, R. N. (2008). First Report of cfr-Mediated Resistance to Linezolid in Human Staphylococcal Clinical Isolates Recovered in the United States. Antimicrob. Agents Chemother. 52: 2244-2246 [Abstract] [Full Text]  
  • Daurel, C., Huet, C., Dhalluin, A., Bes, M., Etienne, J., Leclercq, R. (2008). Differences in Potential for Selection of Clindamycin-Resistant Mutants Between Inducible erm(A) and erm(C) Staphylococcus aureus Genes. J. Clin. Microbiol. 46: 546-550 [Abstract] [Full Text]  
  • Witte, W., Strommenger, B., Cuny, C., Heuck, D., Nuebel, U. (2007). Methicillin-resistant Staphylococcus aureus containing the Panton-Valentine leucocidin gene in Germany in 2005 and 2006. J Antimicrob Chemother 60: 1258-1263 [Abstract] [Full Text]  
  • Holtfreter, S., Grumann, D., Schmudde, M., Nguyen, H. T. T., Eichler, P., Strommenger, B., Kopron, K., Kolata, J., Giedrys-Kalemba, S., Steinmetz, I., Witte, W., Broker, B. M. (2007). Clonal Distribution of Superantigen Genes in Clinical Staphylococcus aureus Isolates. J. Clin. Microbiol. 45: 2669-2680 [Abstract] [Full Text]  
  • Luthje, P., von Kockritz-Blickwede, M., Schwarz, S. (2007). Identification and characterization of nine novel types of small staphylococcal plasmids carrying the lincosamide nucleotidyltransferase gene lnu(A). J Antimicrob Chemother 59: 600-606 [Abstract] [Full Text]  
  • Luthje, P., Schwarz, S. (2007). Molecular analysis of constitutively expressed erm(C) genes selected in vitro in the presence of the non-inducers pirlimycin, spiramycin and tylosin. J Antimicrob Chemother 59: 97-101 [Abstract] [Full Text]  
  • Liao, R. S., Storch, G. A., Buller, R. S., Orscheln, R. C., Mardis, E. R., Armstrong, J. R., Dunne, W. M. Jr. (2006). Blinded Comparison of Repetitive-Sequence PCR and Multilocus Sequence Typing for Genotyping Methicillin-Resistant Staphylococcus aureus Isolates from a Children's Hospital in St. Louis, Missouri.. J. Clin. Microbiol. 44: 2254-2257 [Abstract] [Full Text]  
  • Luthje, P., Schwarz, S. (2006). Antimicrobial resistance of coagulase-negative staphylococci from bovine subclinical mastitis with particular reference to macrolide-lincosamide resistance phenotypes and genotypes. J Antimicrob Chemother 57: 966-969 [Abstract] [Full Text]  
  • Durand, G., Bes, M., Meugnier, H., Enright, M. C., Forey, F., Liassine, N., Wenger, A., Kikuchi, K., Lina, G., Vandenesch, F., Etienne, J. (2006). Detection of New Methicillin-Resistant Staphylococcus aureus Clones Containing the Toxic Shock Syndrome Toxin 1 Gene Responsible for Hospital- and Community-Acquired Infections in France.. J. Clin. Microbiol. 44: 847-853 [Abstract] [Full Text]  
  • Novotna, G., Adamkova, V., Janata, J., Melter, O., Spizek, J. (2005). Prevalence of Resistance Mechanisms against Macrolides and Lincosamides in Methicillin-Resistant Coagulase-Negative Staphylococci in the Czech Republic and Occurrence of an Undefined Mechanism of Resistance to Lincosamides. Antimicrob. Agents Chemother. 49: 3586-3589 [Abstract] [Full Text]  
  • Issartel, B., Tristan, A., Lechevallier, S., Bruyere, F., Lina, G., Garin, B., Lacassin, F., Bes, M., Vandenesch, F., Etienne, J. (2005). Frequent Carriage of Panton-Valentine Leucocidin Genes by Staphylococcus aureus Isolates from Surgically Drained Abscesses. J. Clin. Microbiol. 43: 3203-3207 [Abstract] [Full Text]  
  • Poole, K. (2005). Efflux-mediated antimicrobial resistance. J Antimicrob Chemother 56: 20-51 [Abstract] [Full Text]  
  • Chesneau, O., Ligeret, H., Hosan-Aghaie, N., Morvan, A., Dassa, E. (2005). Molecular Analysis of Resistance to Streptogramin A Compounds Conferred by the Vga Proteins of Staphylococci. Antimicrob. Agents Chemother. 49: 973-980 [Abstract] [Full Text]  
  • Denis, O., Deplano, A., Nonhoff, C., De Ryck, R., de Mendonca, R., Rottiers, S., Vanhoof, R., Struelens, M. J. (2004). National Surveillance of Methicillin-Resistant Staphylococcus aureus in Belgian Hospitals Indicates Rapid Diversification of Epidemic Clones. Antimicrob. Agents Chemother. 48: 3625-3629 [Abstract] [Full Text]  
  • Spiliopoulou, I., Petinaki, E., Papandreou, P., Dimitracopoulos, G. (2004). erm(C) is the predominant genetic determinant for the expression of resistance to macrolides among methicillin-resistant Staphylococcus aureus clinical isolates in Greece. J Antimicrob Chemother 53: 814-817 [Abstract] [Full Text]  
  • Melter, O., Aires de Sousa, M., Urbaskova, P., Jakubu, V., Zemlickova, H., de Lencastre, H. (2003). Update on the Major Clonal Types of Methicillin-Resistant Staphylococcus aureus in the Czech Republic. J. Clin. Microbiol. 41: 4998-5005 [Abstract] [Full Text]  
  • Fiebelkorn, K. R., Crawford, S. A., McElmeel, M. L., Jorgensen, J. H. (2003). Practical Disk Diffusion Method for Detection of Inducible Clindamycin Resistance in Staphylococcus aureus and Coagulase-Negative Staphylococci. J. Clin. Microbiol. 41: 4740-4744 [Abstract] [Full Text]  
  • Kodjikian, L., Burillon, C., Lina, G., Roques, C., Pellon, G., Freney, J., Renaud, F. N. R. (2003). Biofilm Formation on Intraocular Lenses by a Clinical Strain Encoding the ica Locus: A Scanning Electron Microscopy Study. IOVS 44: 4382-4387 [Abstract] [Full Text]  
  • Kodjikian, L., Burillon, C., Roques, C., Pellon, G., Freney, J., Renaud, F. N. R. (2003). Bacterial Adherence of Staphylococcus Epidermidis to Intraocular Lenses: A Bioluminescence and Scanning Electron Microscopy Study. IOVS 44: 4388-4394 [Abstract] [Full Text]  
  • Strommenger, B., Kettlitz, C., Werner, G., Witte, W. (2003). Multiplex PCR Assay for Simultaneous Detection of Nine Clinically Relevant Antibiotic Resistance Genes in Staphylococcus aureus. J. Clin. Microbiol. 41: 4089-4094 [Abstract] [Full Text]  
  • Butaye, P., Devriese, L. A., Haesebrouck, F. (2003). Antimicrobial Growth Promoters Used in Animal Feed: Effects of Less Well Known Antibiotics on Gram-Positive Bacteria. Clin. Microbiol. Rev. 16: 175-188 [Abstract] [Full Text]  
  • Lina, G., Boutite, F., Tristan, A., Bes, M., Etienne, J., Vandenesch, F. (2003). Bacterial Competition for Human Nasal Cavity Colonization: Role of Staphylococcal agr Alleles. Appl. Environ. Microbiol. 69: 18-23 [Abstract] [Full Text]  
  • Denis, O., Magdalena, J., Deplano, A., Nonhoff, C., Hendrickx, E., Struelens, M. J. (2002). Molecular epidemiology of resistance to macrolides-lincosamides-streptogramins in methicillin-resistant Staphylococcusaureus (MRSA) causing bloodstream infections in patients admitted to Belgian hospitals. J Antimicrob Chemother 50: 755-757 [Full Text]  
  • Prunier, A.-L., Malbruny, B., Tande, D., Picard, B., Leclercq, R. (2002). Clinical Isolates of Staphylococcus aureus with Ribosomal Mutations Conferring Resistance to Macrolides. Antimicrob. Agents Chemother. 46: 3054-3056 [Abstract] [Full Text]  
  • Pavie, J., Lefort, A., Zarrouk, V., Chau, F., Garry, L., Leclercq, R., Fantin, B. (2002). Efficacies of Quinupristin-Dalfopristin Combined with Vancomycin In Vitro and in Experimental Endocarditis Due to Methicillin-Resistant Staphylococcus aureus in Relation to Cross-Resistance to Macrolides, Lincosamides, and Streptogramin B- Type Antibiotics. Antimicrob. Agents Chemother. 46: 3061-3064 [Abstract] [Full Text]  
  • Batard, E., Jacqueline, C., Boutoille, D., Hamel, A., Drugeon, H. B., Asseray, N., Leclercq, R., Caillon, J., Potel, G., Bugnon, D. (2002). Combination of Quinupristin-Dalfopristin and Gentamicin against Methicillin-Resistant Staphylococcus aureus: Experimental Rabbit Endocarditis Study. Antimicrob. Agents Chemother. 46: 2174-2178 [Abstract] [Full Text]  
  • Malbruny, B., Canu, A., Bozdogan, B., Fantin, B., Zarrouk, V., Dutka-Malen, S., Feger, C., Leclercq, R. (2002). Resistance to Quinupristin-Dalfopristin Due to Mutation of L22 Ribosomal Protein in Staphylococcus aureus. Antimicrob. Agents Chemother. 46: 2200-2207 [Abstract] [Full Text]  
  • Dominguez, E., Zarazaga, M., Torres, C. (2002). Antibiotic Resistance in Staphylococcus Isolates Obtained from Fecal Samples of Healthy Children. J. Clin. Microbiol. 40: 2638-2641 [Abstract] [Full Text]  
  • Schmitz, F.-J., Petridou, J., Jagusch, H., Astfalk, N., Scheuring, S., Schwarz, S. (2002). Molecular characterization of ketolide-resistant erm(A)-carrying Staphylococcus aureus isolates selected in vitro by telithromycin, ABT-773, quinupristin and clindamycin. J Antimicrob Chemother 49: 611-617 [Abstract] [Full Text]  
  • Lim, J.-A., Kwon, A.-R., Kim, S.-K., Chong, Y., Lee, K., Choi, E.-C. (2002). Prevalence of resistance to macrolide, lincosamide and streptogramin antibiotics in Gram-positive cocci isolated in a Korean hospital. J Antimicrob Chemother 49: 489-495 [Abstract] [Full Text]  
  • Dufour, P., Jarraud, S., Vandenesch, F., Greenland, T., Novick, R. P., Bes, M., Etienne, J., Lina, G. (2002). High Genetic Variability of the agr Locus in Staphylococcus Species. J. Bacteriol. 184: 1180-1186 [Abstract] [Full Text]  
  • Teng, L.-J., Hsueh, P.-R., Ho, S.-W., Luh, K.-T. (2001). High Prevalence of Inducible Erythromycin Resistance among Streptococcus bovis Isolates in Taiwan. Antimicrob. Agents Chemother. 45: 3362-3365 [Abstract] [Full Text]  
  • Fluit, A. C., Visser, M. R., Schmitz, F.-J. (2001). Molecular Detection of Antimicrobial Resistance. Clin. Microbiol. Rev. 14: 836-871 [Abstract] [Full Text]  
  • Werner, G., Cuny, C., Schmitz, F.-J., Witte, W. (2001). Methicillin-Resistant, Quinupristin-Dalfopristin-Resistant Staphylococcus aureus with Reduced Sensitivity to Glycopeptides. J. Clin. Microbiol. 39: 3586-3590 [Abstract] [Full Text]  
  • Chopra, I., Roberts, M. (2001). Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance. Microbiol. Mol. Biol. Rev. 65: 232-260 [Abstract] [Full Text]  
  • Zarrouk, V., Bozdogan, B., Leclercq, R., Garry, L., Feger, C., Carbon, C., Fantin, B. (2001). Activities of the Combination of Quinupristin-Dalfopristin with Rifampin In Vitro and in Experimental Endocarditis Due to Staphylococcus aureus Strains with Various Phenotypes of Resistance to Macrolide-Lincosamide-Streptogramin Antibiotics. Antimicrob. Agents Chemother. 45: 1244-1248 [Abstract] [Full Text]  
  • Hamilton-Miller, J. M. T., Shah, S. (2000). Patterns of phenotypic resistance to the macrolide-lincosamide-ketolide-streptogramin group of antibiotics in staphylococci. J Antimicrob Chemother 46: 941-949 [Abstract] [Full Text]  
  • Fuchs, P. C., Barry, A. L., Brown, S. D. (2000). Bactericidal Activity of Quinupristin-Dalfopristin against Staphylococcus aureus: Clindamycin Susceptibility as a Surrogate Indicator. Antimicrob. Agents Chemother. 44: 2880-2882 [Abstract] [Full Text]  
  • Schmitz, F.-J., Sadurski, R., Kray, A., Boos, M., Geisel, R., Kohrer, K., Verhoef, J., Fluit, A. C. (2000). Prevalence of macrolide-resistance genes in Staphylococcus aureus and Enterococcus faecium isolates from 24 European university hospitals. J Antimicrob Chemother 45: 891-894 [Abstract] [Full Text]  
  • Zarrouk, V., Bozdogan, B., Leclercq, R., Garry, L., Carbon, C., Fantin, B. (2000). Influence of Resistance to Streptogramin A Type Antibiotics on the Activity of Quinupristin-Dalfopristin In Vitro and in Experimental Endocarditis Due to Staphylococcus aureus. Antimicrob. Agents Chemother. 44: 1168-1173 [Abstract] [Full Text]  
  • Portillo, A., Ruiz-Larrea, F., Zarazaga, M., Alonso, A., Martinez, J. L., Torres, C. (2000). Macrolide Resistance Genes in Enterococcus spp.. Antimicrob. Agents Chemother. 44: 967-971 [Abstract] [Full Text]  
  • Bozdogan, B., Leclercq, R. (1999). Effects of Genes Encoding Resistance to Streptogramins A and B on the Activity of Quinupristin-Dalfopristin against Enterococcus faecium. Antimicrob. Agents Chemother. 43: 2720-2725 [Abstract] [Full Text]