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Antimicrobial Agents and Chemotherapy, May 1999, p. 1062-1066, Vol. 43, No. 5
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Distribution of Genes Encoding Resistance to Macrolides, Lincosamides, and Streptogramins among Staphylococci

Gerard Lina,1,* Alain Quaglia,1 Marie-Elisabeth Reverdy,1 Roland Leclercq,2 François Vandenesch,1 and Jerome Etienne1

Faculté de Médecine, Laboratoire de Bactériologie, Centre National de Référence des Toxémies à Staphylocoques, EA 1655, 69372 Lyon Cedex 08,1 and Service de Microbiologie, CHU Côte de Nacre, 14033 Caen,2 France

Received 7 October 1998/Returned for modification 15 January 1999/Accepted 11 February 1999

The relative frequency of 10 determinants of resistance to macrolides, lincosamides, and streptogramins was investigated by PCR in a series of 294 macrolide-, lincosamide-, and/or streptogramin-resistant clinical isolates of Staphylococcus aureus and coagulase-negative staphylococci isolated in 1995 from 32 French hospitals. Resistance was mainly due to the presence of ermA or ermC genes, which were detected in 259 strains (88%), in particular those resistant to methicillin (78% of the strains). Macrolide resistance due to msrA was more prevalent in coagulase-negative staphylococci (14.6%) than in S. aureus (2.1%). Genes related to linA/linA' and conferring resistance to lincomycin were detected in one strain of S. aureus and seven strains of coagulase-negative staphylococci. Resistance to pristinamycin and quinupristin-dalfopristin was phenotypically detected in 10 strains of S. aureus and in three strains of coagulase-negative staphylococci; it was always associated with resistance to type A streptogramins encoded by vat or vatB genes and occurred in association with erm genes. The vga gene conferring decreased susceptibility to type A streptogramins was present alone in three strains of coagulase-negative staphylococci and in combination with erm genes in 10 strains of coagulase-negative staphylococci. A combination of vga-vgb-vat and ermA genes was found in a single strain of S. epidermidis.

* Corresponding author. Mailing address: Faculté de Médecine, Laboratoire de Bactériologie, Centre National de Référence des Toxémies à Staphylocoques, Rue Guillaume Paradin, 69372 Lyon Cedex 08, France. Phone: 33-478-77-86-57. Fax: 33-478-77-86-58. E-mail: geralina{at}univ-lyon1.fr.

Antimicrobial Agents and Chemotherapy, May 1999, p. 1062-1066, Vol. 43, No. 5
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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