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Antimicrobial Agents and Chemotherapy, May 1999, p. 1294-1297, Vol. 43, No. 5
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genetic Characterization of Resistance to Extended-Spectrum beta -Lactams in Klebsiella oxytoca Isolates Recovered from Patients with Septicemia at Hospitals in the Stockholm Area

Shang Wei Wu,1,2,* Kathrine Dornbusch,1 and Göran Kronvall1

The Department of Laboratory Medicine, Division of Clinical Microbiology, Karolinska Institute and Karolinska Hospital, 171 76 Stockholm, Sweden,1 and Laboratory of Microbiology, The Rockefeller University, New York, New York 100212

Received 13 August 1998/Returned for modification 29 January 1999/Accepted 10 February 1999

Two beta -lactamase gene regions were characterized by DNA sequencing in eight clinical isolates of Klebsiella oxytoca. The blaOXY-2a region encoded a beta -lactamase nearly identical to OXY-2 (one amino acid residue substituted) and conferred aztreonam and cefuroxime resistance on the K. oxytoca isolates. Overproduction of OXY-2a was caused by a G-to-A substitution of the fifth nucleotide in the -10 consensus sequence of blaOXY-2a. The blaOXY-1a was identified in a susceptible strain, and the OXY-1a enzyme differed from OXY-1 by two amino acid residues.

* Corresponding author. Mailing address: Box 152, The Rockefeller University, 1230 York Ave., New York, NY 10021. Phone: (212) 327-8278. Fax: (212) 327-8688. E-mail: wus{at}rockvax.rockefeller.edu.

Antimicrobial Agents and Chemotherapy, May 1999, p. 1294-1297, Vol. 43, No. 5
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Decre, D., Burghoffer, B., Gautier, V., Petit, J.-C., Arlet, G. (2004). Outbreak of multi-resistant Klebsiella oxytoca involving strains with extended-spectrum {beta}-lactamases and strains with extended-spectrum activity of the chromosomal {beta}-lactamase. J Antimicrob Chemother 54: 881-888 [Abstract] [Full Text]  
  • Granier, S. A., Leflon-Guibout, V., Goldstein, F. W., Nicolas-Chanoine, M.-H. (2003). New Klebsiella oxytoca {beta}-Lactamase Genes blaOXY-3 and blaOXY-4 and a Third Genetic Group of K. oxytoca Based on blaOXY-3. Antimicrob. Agents Chemother. 47: 2922-2928 [Abstract] [Full Text]  
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