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Antimicrobial Agents and Chemotherapy, June 1999, p. 1484-1486, Vol. 43, No. 6
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Subpopulations of Helicobacter pylori Are Responsible for Discrepancies in the Outcome of Nitroimidazole Susceptibility Testing

E. J. van der Wouden,1 A. de Jong,2 J. C. Thijs,1 J. H. Kleibeuker,3 and A. A. van Zwet2,*

Department of Internal Medicine, Bethesda Hospital,1 and Regional Public Health Laboratory Groningen/Drenthe,2 Hoogeveen, and Department of Gastroenterology, University Hospital, Groningen,3 The Netherlands

Received 12 October 1998/Returned for modification 15 January 1999/Accepted 20 March 1999

Metronidazole susceptibility testing by E test was compared to that by disk diffusion for 263 Helicobacter pylori isolates and to that by breakpoint agar dilution for 90 H. pylori isolates. In 5% and 6% of the cases, respectively, results were discrepant. For each of 52 clinical isolates an E test was performed on 10 separate colonies. Subpopulations of resistant and susceptible bacteria were found in five cases. From three isolates, each colony was subcultured and tested up to 10 times. All but 1 of 292 tests showed the same result. We conclude that the E test is reliable and that subpopulations are responsible for discordant results.

* Corresponding author. Mailing address: Regional Public Health Laboratory Groningen/Drenthe, Bethesda Hospital, Dr. G. H. Amshoffweg 1, 7909 AA Hoogeveen, The Netherlands. Phone: 31 (0) 528-286222. Fax: 31 (0) 528-286435. E-mail: aavzwet{at}wxs.nl.

Antimicrobial Agents and Chemotherapy, June 1999, p. 1484-1486, Vol. 43, No. 6
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.