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Antimicrobial Agents and Chemotherapy, July 1999, p. 1595-1599, Vol. 43, No. 7
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Antimicrobial Susceptibility and Composition of Microcosm Dental Plaques Supplemented with Sucrose

J. Pratten* and M. Wilson

Department of Microbiology, Eastman Dental Institute for Oral Health Care Sciences, University College London, London, WC1X 8LD, United Kingdom

Received 2 February 1999/Returned for modification 26 March 1999/Accepted 4 May 1999

The aims of this study were to evaluate the effects of repeated chlorhexidine gluconate (CHG) pulsing on the viability and bacterial composition of microcosm dental plaques derived from human saliva. The biofilms were grown on bovine enamel discs in a constant-depth film fermentor fed with an artificial saliva which was supplemented thrice daily with sucrose. The microcosm plaques had total viable anaerobic counts of 5 × 108 CFU per mm2 and consisted of 12% Actinomyces spp., 85% streptococci, and 0.2% Veillonella spp. When pulsed twice daily with 0.2% CHG, there was an immediate 1.3-log10 reduction in the total viable (anaerobic) count. However, as pulsing continued, the viable counts recovered, and after 4 days, the anaerobic count reached its pre-CHG-pulsing level, although the bacterial composition of the biofilms had changed. The results of this study show that twice-daily pulsing with 0.2% CHG over a 4-day period was ineffective at reducing the total anaerobic viable count of the biofilms but did alter their bacterial composition.

* Corresponding author. Mailing address: Department of Microbiology, Eastman Dental Institute for Oral Health Care Sciences, University College London, 256 Grays Inn Rd., London, WC1X 8LD, United Kingdom. Phone: 44 (0) 171 915 1107. Fax: 44 (0) 171 915 1127. E-mail: jpratten{at}eastman.ucl.ac.uk.

Antimicrobial Agents and Chemotherapy, July 1999, p. 1595-1599, Vol. 43, No. 7
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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