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Antimicrobial Agents and Chemotherapy, July 1999, p. 1595-1599, Vol. 43, No. 7
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Antimicrobial Susceptibility and Composition of
Microcosm Dental Plaques Supplemented with Sucrose
J.
Pratten* and
M.
Wilson
Department of Microbiology, Eastman Dental
Institute for Oral Health Care Sciences, University College London,
London, WC1X 8LD, United Kingdom
Received 2 February 1999/Returned for modification 26 March
1999/Accepted 4 May 1999
The aims of this study were to evaluate the effects of repeated
chlorhexidine gluconate (CHG) pulsing on the viability and bacterial
composition of microcosm dental plaques derived from human saliva. The
biofilms were grown on bovine enamel discs in a constant-depth film
fermentor fed with an artificial saliva which was supplemented thrice
daily with sucrose. The microcosm plaques had total viable anaerobic
counts of 5 × 108 CFU per mm2 and
consisted of 12% Actinomyces spp., 85% streptococci, and 0.2% Veillonella spp. When pulsed twice daily with 0.2%
CHG, there was an immediate 1.3-log10 reduction in the
total viable (anaerobic) count. However, as pulsing continued, the
viable counts recovered, and after 4 days, the anaerobic count reached
its pre-CHG-pulsing level, although the bacterial composition of the
biofilms had changed. The results of this study show that twice-daily
pulsing with 0.2% CHG over a 4-day period was ineffective at reducing the total anaerobic viable count of the biofilms but did alter their
bacterial composition.
*
Corresponding author. Mailing address: Department of
Microbiology, Eastman Dental Institute for Oral Health Care Sciences, University College London, 256 Grays Inn Rd., London, WC1X 8LD, United
Kingdom. Phone: 44 (0) 171 915 1107. Fax: 44 (0) 171 915 1127. E-mail:
jpratten{at}eastman.ucl.ac.uk.
Antimicrobial Agents and Chemotherapy, July 1999, p. 1595-1599, Vol. 43, No. 7
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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