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Antimicrobial Agents and Chemotherapy, July 1999, p. 1657-1661, Vol. 43, No. 7
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Characterization of TEM-59 (IRT-17), a Novel Inhibitor-Resistant TEM-Derived beta -Lactamase in a Clinical Isolate of Klebsiella oxytoca

H. Bermudes,1 F. Jude,1 E. B. Chaibi,2 C. Arpin,1 C. Bebear,3 R. Labia,2 and C. Quentin1,*

Laboratoire de Microbiologie, UFR des Sciences Pharmaceutiques, Université de Bordeaux 2,1 and Laboratoire de Bactériologie, Hôpital Pellegrin,3 33076 Bordeaux Cedex, and UMR 175 CNRS-MNHN, 29000 Quimper,2 France

Received 16 November 1998/Returned for modification 26 February 1999/Accepted 10 May 1999

A clinical isolate of Klebsiella oxytoca (Kox 443) was found to have a low-level resistance to broad-spectrum penicillins (MICs of amoxicillin and ticarcillin, 256 and 32 µg/ml, respectively), without substantial potentiation by 2 µg of clavulanic acid per ml (amoxicillin- and ticarcillin-clavulanate, 128 and 8 µg/ml, respectively), while being fully susceptible to cephalosporins and other beta -lactam antibiotics. These resistances were carried by a ca. 50-kb conjugative plasmid that encodes a single beta -lactamase with a pI of 5.6. Compared to TEM-2, this enzyme exhibited a 3- to 30-fold higher Km and a decreased maximal hydrolysis rate for beta -lactams; higher concentrations of suicide inactivators (5- to 500-fold higher concentrations giving a 50% reduction in hydrolysis) were required for inhibition. Nucleotide sequence analysis revealed identity between the blaTEM gene of Kox 443 and the blaTEM-2 gene, except for a single A-to-G change at position 590, leading to the amino acid change from Ser-130 Gly. This mutation has not been reported previously in the TEM type beta -lactamases produced by clinical strains, and the novel enzyme was called TEM-59 (alternative name IRT-17). This is the first description of an inhibitor-resistant TEM-derived enzyme in the species K. oxytoca.

* Corresponding author. Mailing address: Laboratoire de Microbiologie, Université de Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France. Phone: (33) 5 57 57 10 75. Fax: (33) 5 56 90 90 72. E-mail: claudine.quentin{at}bacterio.u-bordeaux2.fr.

Antimicrobial Agents and Chemotherapy, July 1999, p. 1657-1661, Vol. 43, No. 7
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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