This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Werckenthin, C.
Right arrow Articles by Westh, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Werckenthin, C.
Right arrow Articles by Westh, H.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, July 1999, p. 1681-1685, Vol. 43, No. 7
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Structural Alterations in the Translational Attenuator of Constitutively Expressed ermC Genes

Christiane Werckenthin,1 Stefan Schwarz,1,* and Henrik Westh2,3

Institut für Tierzucht und Tierverhalten der Bundesforschungsanstalt für Landwirtschaft Braunschweig (FAL), 29223 Celle, Germany,1 and Department of Clinical Microbiology, Hvidovre Hospital, 2650 Hvidovre,2 and Staphylococcus Laboratory, Statens Serum Institut, 2300 Copenhagen,3 Denmark

Received 13 October 1998/Returned for modification 28 December 1998/Accepted 4 May 1999

Sequence deletions of 16, 59, and 111 bp as well as a tandem duplication of 272 bp with respect to the corresponding sequence of pT48 were identified in the regulatory regions of constitutively expressed ermC genes. Constitutive ermC gene expression as a consequence of these structural alterations is based on either the prevention of the formation of mRNA secondary structures in the translational attenuator or the preferential formation of those mRNA secondary structures which do not interfere with the translation of the ermC transcripts. A model for the development of sequence deletions in the ermC translational attenuator by homologous recombination is presented and experimentally tested by in vitro selection of constitutively expressed mutants in staphylococcal strains deficient and proficient in homologous recombination.

* Corresponding author. Mailing address: Institut für Tierzucht und Tierverhalten der Bundesforschungsanstalt für Landwirtschaft Braunschweig (FAL), Dörnbergstr. 25-27, 29223 Celle, Germany. Phone: (49) 5141-384673/77. Fax: (49) 5141-381849. E-mail: SCHWARZ{at}KTF.FAL.DE.

Antimicrobial Agents and Chemotherapy, July 1999, p. 1681-1685, Vol. 43, No. 7
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Yilmaz, G., Aydin, K., Iskender, S., Caylan, R., Koksal, I. (2007). Detection and prevalence of inducible clindamycin resistance in staphylococci. J Med Microbiol 56: 342-345 [Abstract] [Full Text]  
  • Depardieu, F., Podglajen, I., Leclercq, R., Collatz, E., Courvalin, P. (2007). Modes and Modulations of Antibiotic Resistance Gene Expression. Clin. Microbiol. Rev. 20: 79-114 [Abstract] [Full Text]  
  • Luthje, P., Schwarz, S. (2007). Molecular analysis of constitutively expressed erm(C) genes selected in vitro in the presence of the non-inducers pirlimycin, spiramycin and tylosin. J Antimicrob Chemother 59: 97-101 [Abstract] [Full Text]  
  • O'Sullivan, M. V. N., Cai, Y., Kong, F., Zeng, X., Gilbert, G. L. (2006). Influence of Disk Separation Distance on Accuracy of the Disk Approximation Test for Detection of Inducible Clindamycin Resistance in Staphylococcus spp.. J. Clin. Microbiol. 44: 4072-4076 [Abstract] [Full Text]  
  • Luthje, P., Schwarz, S. (2006). Antimicrobial resistance of coagulase-negative staphylococci from bovine subclinical mastitis with particular reference to macrolide-lincosamide resistance phenotypes and genotypes. J Antimicrob Chemother 57: 966-969 [Abstract] [Full Text]  
  • Hauschild, T., Luthje, P., Schwarz, S. (2005). Staphylococcal tetracycline-MLSB resistance plasmid pSTE2 is the product of an RSA-mediated in vivo recombination. J Antimicrob Chemother 56: 399-402 [Abstract] [Full Text]  
  • Doktor, S. Z., Shortridge, V. (2005). Differences in the DNA Sequences in the Upstream Attenuator Region of erm(A) in Clinical Isolates of Streptococcus pyogenes and Their Correlation with Macrolide/Lincosamide Resistance. Antimicrob. Agents Chemother. 49: 3070-3072 [Abstract] [Full Text]  
  • Fiebelkorn, K. R., Crawford, S. A., McElmeel, M. L., Jorgensen, J. H. (2003). Practical Disk Diffusion Method for Detection of Inducible Clindamycin Resistance in Staphylococcus aureus and Coagulase-Negative Staphylococci. J. Clin. Microbiol. 41: 4740-4744 [Abstract] [Full Text]  
  • Butaye, P., Devriese, L. A., Haesebrouck, F. (2003). Antimicrobial Growth Promoters Used in Animal Feed: Effects of Less Well Known Antibiotics on Gram-Positive Bacteria. Clin. Microbiol. Rev. 16: 175-188 [Abstract] [Full Text]  
  • Schwarz, S., Kehrenberg, C., Ojo, K. K. (2002). Staphylococcus sciuri Gene erm(33), Encoding Inducible Resistance to Macrolides, Lincosamides, and Streptogramin B Antibiotics, Is a Product of Recombination between erm(C) and erm(A). Antimicrob. Agents Chemother. 46: 3621-3623 [Abstract] [Full Text]  
  • Schmitz, F.-J., Petridou, J., Jagusch, H., Astfalk, N., Scheuring, S., Schwarz, S. (2002). Molecular characterization of ketolide-resistant erm(A)-carrying Staphylococcus aureus isolates selected in vitro by telithromycin, ABT-773, quinupristin and clindamycin. J Antimicrob Chemother 49: 611-617 [Abstract] [Full Text]  
  • Schmitz, F.-J., Witte, W., Werner, G., Petridou, J., Fluit, A. C., Schwarz, S. (2001). Characterization of the translational attenuator of 20 methicillin-resistant, quinupristin/dalfopristin-resistant Staphylococcus aureus isolates with reduced susceptibility to glycopeptides. J Antimicrob Chemother 48: 939-941 [Full Text]  
  • Fluit, A. C., Visser, M. R., Schmitz, F.-J. (2001). Molecular Detection of Antimicrobial Resistance. Clin. Microbiol. Rev. 14: 836-871 [Abstract] [Full Text]  
  • Fines, M., Gueudin, M., Ramon, A., Leclercq, R. (2001). In vitro selection of resistance to clindamycin related to alterations in the attenuator of the erm(TR) gene of Streptococcus pyogenes UCN1 inducibly resistant to erythromycin. J Antimicrob Chemother 48: 411-416 [Abstract] [Full Text]  
  • Chopra, I., Roberts, M. (2001). Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance. Microbiol. Mol. Biol. Rev. 65: 232-260 [Abstract] [Full Text]  
  • Schmitz, F.-J., Petridou, J., Astfalk, N., Scheuring, S., Köhrer, K., Verhoef, J., Fluit, A. C., Schwarz, S. (2001). Structural Alterations in the Translational Attenuator of Constitutively Expressed erm(A) Genes in Staphylococcus aureus. Antimicrob. Agents Chemother. 45: 1603-1604 [Full Text]  
  • Werckenthin, C., Schwarz, S. (2000). Molecular analysis of the translational attenuator of a constitutively expressed erm(A) gene from Staphylococcus intermedius. J Antimicrob Chemother 46: 785-788 [Abstract] [Full Text]  
  • Roberts, M. C., Sutcliffe, J., Courvalin, P., Jensen, L. B., Rood, J., Seppala, H. (1999). Nomenclature for Macrolide and Macrolide-Lincosamide-Streptogramin B Resistance Determinants. Antimicrob. Agents Chemother. 43: 2823-2830 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»