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Antimicrobial Agents and Chemotherapy, July 1999, p. 1779-1782, Vol. 43, No. 7
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Rapid Detection, by PCR and Reverse Hybridization, of Mutations in the Helicobacter pylori 23S rRNA Gene, Associated with Macrolide Resistance

Leen-Jan van Doorn,1,* Yvette J. Debets-Ossenkopp,2 Armelle Marais,3 Ricardo Sanna,1 Francis Mégraud,3 Johannes G. Kusters,2 and Wim G. V. Quint1

Delft Diagnostic Laboratory, Delft,1 and Department of Medical Microbiology, Free University of Amsterdam, Amsterdam,2 The Netherlands, and Laboratoire de Bactériologie, Hôpital Pellegrin and University Victor Ségalen Bordeaux 2, Bordeaux, France3

Received 23 November 1998/Returned for modification 15 February 1999/Accepted 4 May 1999

A PCR-based reverse hybridization system (research prototype kit INNO-LiPA for H. pylori resistance) was developed and evaluated for simultaneous detection of 23S ribosomal DNA point mutations, associated with macrolide resistance in Helicobacter pylori. Fifty-seven H. pylori strains (51 natural, 6 laboratory-derived artificial, 52 resistant, and 5 susceptible strains) were tested by PCR-LiPA (detecting mutations A2115right-arrowG, G2141right-arrowA, A2142right-arrowG, A2142right-arrowC, A2143right-arrowG, A2143right-arrowC, and A2143right-arrowT), DNA sequencing, restriction fragment length polymorphism, and/or hybridization to oligonucleotide probes. Results were highly concordant, but PCR-LiPA appears to be more sensitive for the simultaneous detection of multiple mutants.

* Corresponding author. Mailing address: Delft Diagnostic Laboratory, R. de Graafweg 7, 2625 AD Delft, The Netherlands. Phone: 31-15-2604577. Fax: 31-15-2604550. E-mail: L.J.van.Doorn{at}ddl.nl.

Antimicrobial Agents and Chemotherapy, July 1999, p. 1779-1782, Vol. 43, No. 7
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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