This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Doorn, L.-J.
Right arrow Articles by Quint, W. G. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Doorn, L.-J.
Right arrow Articles by Quint, W. G. V.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, July 1999, p. 1779-1782, Vol. 43, No. 7
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Rapid Detection, by PCR and Reverse Hybridization, of Mutations in the Helicobacter pylori 23S rRNA Gene, Associated with Macrolide Resistance

Leen-Jan van Doorn,1,* Yvette J. Debets-Ossenkopp,2 Armelle Marais,3 Ricardo Sanna,1 Francis Mégraud,3 Johannes G. Kusters,2 and Wim G. V. Quint1

Delft Diagnostic Laboratory, Delft,1 and Department of Medical Microbiology, Free University of Amsterdam, Amsterdam,2 The Netherlands, and Laboratoire de Bactériologie, Hôpital Pellegrin and University Victor Ségalen Bordeaux 2, Bordeaux, France3

Received 23 November 1998/Returned for modification 15 February 1999/Accepted 4 May 1999

A PCR-based reverse hybridization system (research prototype kit INNO-LiPA for H. pylori resistance) was developed and evaluated for simultaneous detection of 23S ribosomal DNA point mutations, associated with macrolide resistance in Helicobacter pylori. Fifty-seven H. pylori strains (51 natural, 6 laboratory-derived artificial, 52 resistant, and 5 susceptible strains) were tested by PCR-LiPA (detecting mutations A2115right-arrowG, G2141right-arrowA, A2142right-arrowG, A2142right-arrowC, A2143right-arrowG, A2143right-arrowC, and A2143right-arrowT), DNA sequencing, restriction fragment length polymorphism, and/or hybridization to oligonucleotide probes. Results were highly concordant, but PCR-LiPA appears to be more sensitive for the simultaneous detection of multiple mutants.


* Corresponding author. Mailing address: Delft Diagnostic Laboratory, R. de Graafweg 7, 2625 AD Delft, The Netherlands. Phone: 31-15-2604577. Fax: 31-15-2604550. E-mail: L.J.van.Doorn{at}ddl.nl.


Antimicrobial Agents and Chemotherapy, July 1999, p. 1779-1782, Vol. 43, No. 7
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Pandori, M. W., Gordones, C., Castro, L., Engelman, J., Siedner, M., Lukehart, S., Klausner, J. (2007). Detection of Azithromycin Resistance in Treponema pallidum by Real-Time PCR. Antimicrob. Agents Chemother. 51: 3425-3430 [Abstract] [Full Text]  
  • Lottspeich, C., Schwarzer, A., Panthel, K., Koletzko, S., Russmann, H. (2007). Evaluation of the Novel Helicobacter pylori ClariRes Real-Time PCR Assay for Detection and Clarithromycin Susceptibility Testing of H. pylori in Stool Specimens from Symptomatic Children. J. Clin. Microbiol. 45: 1718-1722 [Abstract] [Full Text]  
  • Megraud, F., Lehours, P. (2007). Helicobacter pylori Detection and Antimicrobial Susceptibility Testing. Clin. Microbiol. Rev. 20: 280-322 [Abstract] [Full Text]  
  • Gibreel, A., Taylor, D. E. (2006). Macrolide resistance in Campylobacter jejuni and Campylobacter coli. J Antimicrob Chemother 58: 243-255 [Abstract] [Full Text]  
  • Posteraro, P., Branca, G., Sanguinetti, M., Ranno, S., Cammarota, G., Rahimi, S., De Carlo, M., Posteraro, B., Fadda, G. (2006). Rapid detection of clarithromycin resistance in Helicobacter pylori using a PCR-based denaturing HPLC assay. J Antimicrob Chemother 57: 71-78 [Abstract] [Full Text]  
  • Schabereiter-Gurtner, C., Hirschl, A. M., Dragosics, B., Hufnagl, P., Puz, S., Kovach, Z., Rotter, M., Makristathis, A. (2004). Novel Real-Time PCR Assay for Detection of Helicobacter pylori Infection and Simultaneous Clarithromycin Susceptibility Testing of Stool and Biopsy Specimens. J. Clin. Microbiol. 42: 4512-4518 [Abstract] [Full Text]  
  • Megraud, F (2004). H pylori antibiotic resistance: prevalence, importance, and advances in testing. Gut 53: 1374-1384 [Full Text]  
  • Oleastro, M., Menard, A., Santos, A., Lamouliatte, H., Monteiro, L., Barthelemy, P., Megraud, F. (2003). Real-Time PCR Assay for Rapid and Accurate Detection of Point Mutations Conferring Resistance to Clarithromycin in Helicobacter pylori. J. Clin. Microbiol. 41: 397-402 [Abstract] [Full Text]  
  • WOLLE, K., LEODOLTER, A., MALFERTHEINER, P., KONIG, W. (2002). Antibiotic susceptibility of Helicobacter pylori in Germany: stable primary resistance from 1995 to 2000. J Med Microbiol 51: 705-709 [Abstract] [Full Text]  
  • Menard, A., Santos, A., Megraud, F., Oleastro, M. (2002). PCR-Restriction Fragment Length Polymorphism Can Also Detect Point Mutation A2142C in the 23S rRNA Gene, Associated with Helicobacter pylori Resistance to Clarithromycin. Antimicrob. Agents Chemother. 46: 1156-1157 [Full Text]  
  • Owen, R J (2002). Molecular testing for antibiotic resistance in Helicobacter pylori. Gut 50: 285-289 [Abstract] [Full Text]  
  • Israel, D. A., Salama, N., Krishna, U., Rieger, U. M., Atherton, J. C., Falkow, S., Peek, R. M. Jr. (2001). Helicobacter pylori genetic diversity within the gastric niche of a single human host. Proc. Natl. Acad. Sci. USA 10.1073/pnas.251551698v1 [Abstract] [Full Text]  
  • Fluit, A. C., Visser, M. R., Schmitz, F.-J. (2001). Molecular Detection of Antimicrobial Resistance. Clin. Microbiol. Rev. 14: 836-871 [Abstract] [Full Text]  
  • van der Ende, A., van Doorn, L.-J., Rooijakkers, S., Feller, M., Tytgat, G. N. J., Dankert, J. (2001). Clarithromycin-Susceptible and -Resistant Helicobacter pylori Isolates with Identical Randomly Amplified Polymorphic DNA-PCR Genotypes Cultured from Single Gastric Biopsy Specimens Prior to Antibiotic Therapy. J. Clin. Microbiol. 39: 2648-2651 [Abstract] [Full Text]  
  • van Doorn, L.-J., Glupczynski, Y., Kusters, J. G., Mégraud, F., Midolo, P., Maggi-Solcà, N., Queiroz, D. M. M., Nouhan, N., Stet, E., Quint, W. G. V. (2001). Accurate Prediction of Macrolide Resistance in Helicobacter pylori by a PCR Line Probe Assay for Detection of Mutations in the 23S rRNA Gene: Multicenter Validation Study. Antimicrob. Agents Chemother. 45: 1500-1504 [Abstract] [Full Text]  
  • Ryan, K. A., van Doorn, L.-J., Moran, A. P., Glennon, M., Smith, T., Maher, M. (2001). Evaluation of Clarithromycin Resistance and cagA and vacA Genotyping of Helicobacter pylori Strains from the West of Ireland Using Line Probe Assays. J. Clin. Microbiol. 39: 1978-1980 [Abstract] [Full Text]  
  • Vester, B., Douthwaite, S. (2001). Macrolide Resistance Conferred by Base Substitutions in 23S rRNA. Antimicrob. Agents Chemother. 45: 1-12 [Full Text]  
  • Bollhalder, M., Mura, C., Landt, O., Maly, F. E. (1999). LightCycler PCR Assay for Simultaneous Detection of the H63D and S65C Mutations in the HFE Hemochromatosis Gene Based on Opposite Melting Temperature Shifts. Clin. Chem. 45: 2275-2278 [Full Text]  
  • Israel, D. A., Salama, N., Krishna, U., Rieger, U. M., Atherton, J. C., Falkow, S., Peek, R. M. Jr. (2001). Helicobacter pylori genetic diversity within the gastric niche of a single human host. Proc. Natl. Acad. Sci. USA 98: 14625-14630 [Abstract] [Full Text]