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Antimicrobial Agents and Chemotherapy, September 1999, p. 2161-2164, Vol. 43, No. 9
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

VanE, a New Type of Acquired Glycopeptide Resistance in Enterococcus faecalis BM4405

Marguerite Fines,1,dagger Bruno Perichon,1,* Peter Reynolds,2 Daniel F. Sahm,3 and Patrice Courvalin1

Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15, France1; Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom2; and MRL Pharmaceutical Services, Herndon, Virginia 201713

Received 25 March 1999/Returned for modification 2 June 1999/Accepted 21 June 1999

Enterococcus faecalis BM4405 was resistant to low levels of vancomycin (MIC, 16 µg/ml) and was susceptible to teicoplanin (MIC, 0.5 µg/ml). No PCR product was obtained when the total DNA of this clinical isolate was used as a template with primers specific for glycopeptide resistance genes vanA, vanB, vanC, and vanD. However, a 604-bp PCR fragment was obtained when V1 and V2 degenerate primers were used and total DNA was digested with HindIII as a template. The product was cloned and sequenced. The deduced amino acid sequence had greater identity (55%) with VanC than with VanA (45%), VanB (43%), or VanD (44%). This was consistent with the fact that BM4405 synthesized peptidoglycan precursors that terminated in D-serine residues. After induction with vancomycin, weak D,D-dipeptidase and penicillin-insensitive D,D-carboxypeptidase activities were detected in cytoplasmic extracts of BM4405, whereas a serine racemase activity was found in the membrane preparation. This new type of acquired glycopeptide resistance was named VanE.

* Corresponding author. Mailing address: Unité des Agents Antibactériens, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: (33) (1) 45 68 83 18. Fax: (33) (1) 45 68 83 19. E-mail: brunoper{at}pasteur.fr.

dagger Present address: Laboratoire de Microbiologie, CHU de la Côte de Nacre, 14033 Caen Cedex, France.

Antimicrobial Agents and Chemotherapy, September 1999, p. 2161-2164, Vol. 43, No. 9
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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