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Antimicrobial Agents and Chemotherapy, September 1999, p. 2176-2182, Vol. 43, No. 9
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Structural Gene for Microcin H47 Encodes a Peptide Precursor with Antibiotic Activity

Eliana Rodríguez, Carina Gaggero,dagger and Magela Laviña*

Sección de Fisiología y Genética Bacterianas, Facultad de Ciencias, Iguá 4225, Malvín Norte, Montevideo, Uruguay

Received 8 June 1999/Accepted 14 June 1999

Microcin H47 is a bactericidal antibiotic produced by a naturally occurring Escherichia coli strain isolated in Uruguay. The microcin genetic system is located in the chromosome and extends over a 10-kb DNA segment containing the genes required for microcin synthesis, secretion, and immunity. The smallest microcin synthesis gene, mchB, was sequenced and shown to encode a highly hydrophobic peptide. An mchB-phoA gene fusion, which directed the synthesis of a hybrid bifunctional protein with both PhoA and microcin H47-like activities, was isolated. The results presented herein lead us to propose that microcin H47 is indeed a ribosomally synthesized peptide antibiotic and that its peptide precursor already has antibiotic activity of the same specificity as that of mature microcin.

* Corresponding author. Mailing address: Sección de Fisiología y Genética Bacterianas, Facultad de Ciencias, Iguá 4225, Malvín Norte, Montevideo, Uruguay. Phone: (5982) 525 86 29. Fax: (5982) 525 86 29. E-mail: magela{at}fcien.edu.uy.

dagger Present address: División de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo 11600, Uruguay.

Antimicrobial Agents and Chemotherapy, September 1999, p. 2176-2182, Vol. 43, No. 9
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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