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Antimicrobial Agents and Chemotherapy, September 1999, p. 2317-2319, Vol. 43, No. 9
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Relationship between Pyrazinamide Resistance, Loss of Pyrazinamidase Activity, and Mutations in the pncA Locus in Multidrug-Resistant Clinical Isolates of Mycobacterium tuberculosis

M. Mestdagh,1 P. A. Fonteyne,2 L. Realini,2 R. Rossau,3 G. Jannes,3 W. Mijs,3 K. A. L. De Smet,3 F. Portaels,2 and E. Van den Eeckhout1,*

Laboratory for Pharmaceutical Biotechnology, FFW, University of Ghent, 9000 Ghent,1 Unit of Mycobacteriology, Institute of Tropical Medicine, 2000 Antwerp,2 and Innogenetics n.v., 9052 Ghent,3 Belgium

Received 25 March 1999/Returned for modification 16 June 1999/Accepted 13 July 1999

Sixty-two Mycobacterium tuberculosis isolates were tested for pyrazinamidase activity, and their pyrazinamide susceptibility was determined by the radiometric method. Sequencing of pncA genes in the 23 resistant strains revealed mutations in 16 pyrazinamidase-negative strains, 11 of which had not been previously described. Six isolates containing wild-type pncA might possess alternative resistance mechanisms.

* Corresponding author. Mailing address: Laboratory for Pharmaceutical Biotechnology, FFW, University of Ghent, Harelbekestraat 72, 9000 Ghent, Belgium. Phone: 32-9.264.80.52. Fax: 32-9.220.66.88. E-mail: Elfriede.VandenEeckhout{at}rug.ac.be.

Antimicrobial Agents and Chemotherapy, September 1999, p. 2317-2319, Vol. 43, No. 9
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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