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Antimicrobial Agents and Chemotherapy, January 2000, p. 134-138, Vol. 44, No. 1
0066-4804/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Chemical Specificity of the PDR5 Multidrug Resistance Gene Product of Saccharomyces cerevisiae Based on Studies with Tri-n-Alkyltin Chlorides

John Golin,1,* Alisa Barkatt,1 Susan Cronin,1,3 George Eng,4 and Leopold May2

Departments of Biology1 and Chemistry,2 The Catholic University of America, Washington, D.C. 20064; Department of Biology, Immaculata College, Immaculata, Pennsylvania 193453; and Department of Chemistry and Physics, University of the District of Columbia, Washington, D.C. 200084

Received 15 July 1999/Returned for modification 16 September 1999/Accepted 12 October 1999

To understand the chemical basis of action for the PDR5-encoded multidrug resistance transporter of Saccharomyces cerevisiae, we compared the relative hypersensitivities of the wild-type (RW2802) and null mutant strains toward a series of tri-n-alkyltin compounds. These compounds differ from each other in a systematic fashion---either by hydrocarbon chain length or by anion composition. Using zone-of-inhibition and fixed-concentration assays, we found that the ethyl, propyl, and butyl compounds are strong PDR5 substrates, whereas the methyl and pentyl compounds are weak. We conclude that hydrophobicity and anion makeup are relatively unimportant factors in determining whether a tri-n-alkyltin compound is a good PDR5 substrate but that the dissociation of the compound and the molecular size are significant.

* Corresponding author. Mailing address: Department of Biology, The Catholic University of America, Washington, DC 20064. Phone: (202) 319-5722. Fax: (202) 319-5721. E-mail: Golin{at}cua.edu.

Antimicrobial Agents and Chemotherapy, January 2000, p. 134-138, Vol. 44, No. 1
0066-4804/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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