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Antimicrobial Agents and Chemotherapy, October 2000, p. 2684-2688, Vol. 44, No. 10
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Characterization of Integrons in Acinetobacter baumannii: Description of a Hybrid Class 2 Integron

Marie-Cécile Ploy,1,2,* François Denis,1 Patrice Courvalin,2 and Thierry Lambert2,3

Laboratoire de Bactériologie-Virologie-Hygiène, CHU Dupuytren, 87042 Limoges,1 Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15,2 and Centre d'Etudes Pharmaceutiques, 92296 Châtenay-Malabry,3 France

Received 15 December 1999/Returned for modification 28 March 2000/Accepted 1 July 2000

Twenty Acinetobacter baumannii strains resistant to various antibiotics were analyzed for integron content and sequences of the amplification products. Sixteen clinical isolates had a class 1 integron, 2 contained an additional class 1 or class 2 integron, but no class 3 integron was detected. Thirteen strains had integrons with a single cassette: aac(3)-Ia (9 strains), ant(2")-Ia (2 strains), or aac(6')-Ib (2 strains); 1 had aac(6')-Ib and oxa20 cassettes and an unknown gene; and 1 had an integron containing ant(2")-Ia and an oxa3 cassette truncated by IS6100. The remaining strains harbored class 1 integrons with gene cassettes previously found in Enterobacteriaceae. One integron had a hybrid structure composed of intI2 and the 3' conserved segment of class 1 integrons. These data indicate that integrons play a major role in multidrug resistance in Acinetobacter.

* Corresponding author. Mailing address: Laboratoire de Bactériologie-Virologie-Hygiène, CHU Dupuytren, 2, Avenue Martin Luther King, 87042 Limoges Cedex, France. Phone: (33) (5) 55 05 61 66. Fax: (33) (5) 55 05 67 22. E-mail: marieploy{at}lemel.fr.

Antimicrobial Agents and Chemotherapy, October 2000, p. 2684-2688, Vol. 44, No. 10
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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