Molecular Characterization of Integrons in Acinetobacter baumannii: Description of a Hybrid Class 2 Integron

doi:10.1128/AAC.44.10.2684-2688.2000

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Antimicrobial Agents and Chemotherapy, October 2000, p. 2684-2688, Vol. 44, No. 10
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Characterization of Integrons in Acinetobacter baumannii: Description of a Hybrid Class 2 Integron

Marie-Cécile Ploy,¹^,²^,^* François Denis,¹ Patrice Courvalin,² and Thierry Lambert²^,³

Laboratoire de Bactériologie-Virologie-Hygiène, CHU Dupuytren, 87042 Limoges,¹ Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15,² and Centre d'Etudes Pharmaceutiques, 92296 Châtenay-Malabry,³ France

Received 15 December 1999/Returned for modification 28 March 2000/Accepted 1 July 2000

Twenty Acinetobacter baumannii strains resistant to various antibiotics were analyzed for integron content and sequences ofthe amplification products. Sixteen clinical isolates had a class1 integron, 2 contained an additional class 1 or class 2 integron,but no class 3 integron was detected. Thirteen strains had integronswith a single cassette: aac(3)-Ia (9 strains), ant(2")-Ia (2 strains),or aac(6')-Ib (2 strains); 1 had aac(6')-Ib and oxa20 cassettesand an unknown gene; and 1 had an integron containing ant(2")-Iaand an oxa3 cassette truncated by IS6100. The remaining strainsharbored class 1 integrons with gene cassettes previously foundin Enterobacteriaceae. One integron had a hybrid structure composedof intI2 and the 3' conserved segment of class 1 integrons. Thesedata indicate that integrons play a major role in multidrug resistancein Acinetobacter.

^* Corresponding author. Mailing address: Laboratoire de Bactériologie-Virologie-Hygiène, CHU Dupuytren, 2, Avenue Martin LutherKing, 87042 Limoges Cedex, France. Phone: (33) (5) 55 05 61 66.Fax: (33) (5) 55 05 67 22. E-mail: marieploy{at}lemel.fr.

Antimicrobial Agents and Chemotherapy, October 2000, p. 2684-2688, Vol. 44, No. 10
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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