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Antimicrobial Agents and Chemotherapy, October 2000, p. 2701-2705, Vol. 44, No. 10
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

In Vitro Antimicrobial Properties of Recombinant ASABF, an Antimicrobial Peptide Isolated from the Nematode Ascaris suum

Hong Zhang,1,2 Shigenobu Yoshida,1 Tomoyasu Aizawa,3 Ritsuko Murakami,1 Masato Suzuki,3 Nozomu Koganezawa,3 Atsushi Matsuura,3 Mitsuhiro Miyazawa,1 Keiichi Kawano,3,dagger Katsutoshi Nitta,3 and Yusuke Kato1,*

National Institute of Sericultural and Entomological Science, Tsukuba,1 Graduate School of Science, Hokkaido University, Sapporo,3 and Department of Pediatrics, University of Tsukuba, Ibaraki,2 Japan

Received 5 April 2000/Returned for modification 29 May 2000/Accepted 5 July 2000

ASABF is a CSalpha beta -type antimicrobial peptide that contains four intramolecular disulfide bridges (Y. Kato and S. Komatsu, J. Biol. Chem. 271:30493-30498, 1996). In the present study, a recombinant ASABF was produced by using a yeast expression system, and its antimicrobial activity was characterized in detail. The recombinant ASABF was active against all gram-positive bacteria tested (7 of 7; minimum bactericidal concentration [MBC], 0.03 to 1 µg/ml) except Leuconostoc mesenteroides, some gram-negative bacteria (8 of 14; MBC, >0.5 µg/ml), and some yeasts (3 of 9; MBC >3 µg/ml). Slight hemolytic activity (4.2% at 100 µg/ml) against human erythrocytes was observed only under low-ionic-strength conditions. Less than 1 min of contact was enough to kill Staphylococcus aureus ATCC 6538P. The bactericidal activity against S. aureus was inhibited by salts.

* Corresponding author. Mailing address: Laboratory of Metabolism, National Institute of Sericultural and Entomological Science, Oowashi 1-2, Tsukuba, Ibaraki 305-8634, Japan. Phone: 81-298-38-6106. Fax: 81-298-38-6028. E-mail: kato{at}nises.affrc.go.jp.

dagger Present address: Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Toyama, Japan.

Antimicrobial Agents and Chemotherapy, October 2000, p. 2701-2705, Vol. 44, No. 10
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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