Animal and Human Multidrug-Resistant, Cephalosporin-Resistant Salmonella Isolates Expressing a Plasmid-Mediated CMY-2 AmpC beta -Lactamase

doi:10.1128/AAC.44.10.2777-2783.2000

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Winokur, P. L.
	Articles by Doern, G. V.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Winokur, P. L.
	Articles by Doern, G. V.

Previous Article | Next Article

Antimicrobial Agents and Chemotherapy, October 2000, p. 2777-2783, Vol. 44, No. 10
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Animal and Human Multidrug-Resistant, Cephalosporin-Resistant Salmonella Isolates Expressing a Plasmid-Mediated CMY-2 AmpC $beta$ -Lactamase

P. L. Winokur,¹^,²^,^* A. Brueggemann,¹ D. L. DeSalvo,² L. Hoffmann,³ M. D. Apley,³ E. K. Uhlenhopp,³ M. A. Pfaller,¹ and G. V. Doern¹

University of Iowa College of Medicine¹ and The Veterans Affairs Medical Center,² Iowa City, and Iowa State College of Veterinary Medicine, Ames,³ Iowa

Received 18 February 2000/Returned for modification 21 June 2000/Accepted 25 July 2000

Salmonella spp. are important food-borne pathogens that are demonstrating increasing antimicrobial resistance rates in isolatesobtained from food animals and humans. In this study, 10 multidrug-resistant,cephalosporin-resistant Salmonella isolates from bovine, porcine,and human sources from a single geographic region were identified.All isolates demonstrated resistance to cephamycins and extended-spectrumcephalosporins as well as tetracycline, chloramphenicol, streptomycin,and sulfisoxazole. Molecular epidemiological analyses revealedeight distinct chromosomal DNA patterns, suggesting that clonalspread could not entirely explain the distribution of this antimicrobialresistance phenotype. However, all isolates encoded an AmpC-like $beta$ -lactamase, CMY-2. Eight isolates contained a large nonconjugativeplasmid that could transform Escherichia coli. Transformants coexpressedcephalosporin, tetracycline, chloramphenicol, streptomycin, andsulfisoxazole resistances. Plasmid DNA revealed highly relatedrestriction fragments though plasmids appeared to have undergonesome evolution over time. Multidrug-resistant, cephalosporin-resistantSalmonella spp. present significant therapeutic problems in animaland human health care and raise further questions about the associationbetween antimicrobial resistance, antibiotic use in animals, andtransfer of multidrug-resistant Salmonella spp. between animalsandman.

^* Corresponding author. Mailing address: SW34 GH, 200 Hawkins Dr., Iowa City, IA 52242. Phone: (319) 356-3909. Fax: (319) 345-4600.E-mail: patricia-winokur{at}uiowa.edu.

Antimicrobial Agents and Chemotherapy, October 2000, p. 2777-2783, Vol. 44, No. 10
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

Kaldhone, P., Nayak, R., Lynne, A. M., David, D. E., McDermott, P. F., Logue, C. M., Foley, S. L. (2008). Characterization of Salmonella enterica serovar Heidelberg from Turkey-Associated Sources. Appl. Environ. Microbiol. 74: 5038-5046 [Abstract] [Full Text]
Wu, J.-J., Ko, W.-C., Wu, H.-M., Yan, J.-J. (2008). Prevalence of Qnr determinants among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae in a Taiwanese Hospital, 1999-2005. J Antimicrob Chemother 61: 1234-1239 [Abstract] [Full Text]
Smet, A., Martel, A., Persoons, D., Dewulf, J., Heyndrickx, M., Catry, B., Herman, L., Haesebrouck, F., Butaye, P. (2008). Diversity of Extended-Spectrum {beta}-Lactamases and Class C {beta}-Lactamases among Cloacal Escherichia coli Isolates in Belgian Broiler Farms. Antimicrob. Agents Chemother. 52: 1238-1243 [Abstract] [Full Text]
Adler, H., Fenner, L., Walter, P., Hohler, D., Schultheiss, E., Oezcan, S., Frei, R. (2008). Plasmid-mediated AmpC {beta}-lactamases in Enterobacteriaceae lacking inducible chromosomal ampC genes: prevalence at a Swiss university hospital and occurrence of the different molecular types in Switzerland. J Antimicrob Chemother 61: 457-458 [Full Text]
Daniels, J. B., Call, D. R., Besser, T. E. (2007). Molecular Epidemiology of blaCMY-2 Plasmids Carried by Salmonella enterica and Escherichia coli Isolates from Cattle in the Pacific Northwest. Appl. Environ. Microbiol. 73: 8005-8011 [Abstract] [Full Text]
Zaidi, M. B., Leon, V., Canche, C., Perez, C., Zhao, S., Hubert, S. K., Abbott, J., Blickenstaff, K., McDermott, P. F. (2007). Rapid and widespread dissemination of multidrug-resistant blaCMY-2 Salmonella Typhimurium in Mexico. J Antimicrob Chemother 60: 398-401 [Abstract] [Full Text]
Sawant, A. A., Hegde, N. V., Straley, B. A., Donaldson, S. C., Love, B. C., Knabel, S. J., Jayarao, B. M. (2007). Antimicrobial-Resistant Enteric Bacteria from Dairy Cattle. Appl. Environ. Microbiol. 73: 156-163 [Abstract] [Full Text]
You, Y., Rankin, S. C., Aceto, H. W., Benson, C. E., Toth, J. D., Dou, Z. (2006). Survival of Salmonella enterica Serovar Newport in Manure and Manure-Amended Soils. Appl. Environ. Microbiol. 72: 5777-5783 [Abstract] [Full Text]
Fonseca, E. L., Mykytczuk, O. L., Asensi, M. D., Reis, E. M. F., Ferraz, L. R., Paula, F. L., Ng, L. K., Rodrigues, D. P. (2006). Clonality and Antimicrobial Resistance Gene Profiles of Multidrug- Resistant Salmonella enterica Serovar Infantis Isolates from Four Public Hospitals in Rio de Janeiro, Brazil.. J. Clin. Microbiol. 44: 2767-2772 [Abstract] [Full Text]
Travis, R. M., Gyles, C. L., Reid-Smith, R., Poppe, C., McEwen, S. A., Friendship, R., Janecko, N., Boerlin, P. (2006). Chloramphenicol and kanamycin resistance among porcine Escherichia coli in Ontario. J Antimicrob Chemother 58: 173-177 [Abstract] [Full Text]
Donaldson, S. C., Straley, B. A., Hegde, N. V., Sawant, A. A., DebRoy, C., Jayarao, B. M. (2006). Molecular Epidemiology of Ceftiofur-Resistant Escherichia coli Isolates from Dairy Calves.. Appl. Environ. Microbiol. 72: 3940-3948 [Abstract] [Full Text]
Yan, J.-J., Hsueh, P.-R., Lu, J.-J., Chang, F.-Y., Shyr, J.-M., Wan, J.-H., Liu, Y.-C., Chuang, Y.-C., Yang, Y.-C., Tsao, S.-M., Wu, H.-H., Wang, L.-S., Lin, T.-P., Wu, H.-M., Chen, H.-M., Wu, J.-J. (2006). Extended-Spectrum {beta}-Lactamases and Plasmid-Mediated AmpC Enzymes among Clinical Isolates of Escherichia coli and Klebsiella pneumoniae from Seven Medical Centers in Taiwan.. Antimicrob. Agents Chemother. 50: 1861-1864 [Abstract] [Full Text]
Sidjabat, H. E., Townsend, K. M., Hanson, N. D., Bell, J. M., Stokes, H. W., Gobius, K. S., Moss, S. M., Trott, D. J. (2006). Identification of blaCMY-7 and associated plasmid-mediated resistance genes in multidrug-resistant Escherichia coli isolated from dogs at a veterinary teaching hospital in Australia. J Antimicrob Chemother 57: 840-848 [Abstract] [Full Text]
Kang, M.-S., Besser, T. E., Call, D. R. (2006). Variability in the Region Downstream of the blaCMY-2 {beta}-Lactamase Gene in Escherichia coli and Salmonella enterica Plasmids.. Antimicrob. Agents Chemother. 50: 1590-1593 [Abstract] [Full Text]
Su, L.-H., Chen, H.-L., Chia, J.-H., Liu, S.-Y., Chu, C., Wu, T.-L., Chiu, C.-H. (2006). Distribution of a transposon-like element carrying blaCMY-2 among Salmonella and other Enterobacteriaceae. J Antimicrob Chemother 57: 424-429 [Abstract] [Full Text]
Pournaras, S., Ikonomidis, A., Tzouvelekis, L. S., Tokatlidou, D., Spanakis, N., Maniatis, A. N., Legakis, N. J., Tsakris, A. (2005). VIM-12, a Novel Plasmid-Mediated Metallo-{beta}-Lactamase from Klebsiella pneumoniae That Resembles a VIM-1/VIM-2 Hybrid. Antimicrob. Agents Chemother. 49: 5153-5156 [Abstract] [Full Text]
Rankin, S. C., Whichard, J. M., Joyce, K., Stephens, L., O'Shea, K., Aceto, H., Munro, D. S., Benson, C. E. (2005). Detection of a blaSHV Extended-Spectrum {beta}-Lactamase in Salmonella enterica Serovar Newport MDR-AmpC. J. Clin. Microbiol. 43: 5792-5793 [Abstract] [Full Text]
Alcaine, S. D., Sukhnanand, S. S., Warnick, L. D., Su, W.-L., McGann, P., McDonough, P., Wiedmann, M. (2005). Ceftiofur-Resistant Salmonella Strains Isolated from Dairy Farms Represent Multiple Widely Distributed Subtypes That Evolved by Independent Horizontal Gene Transfer. Antimicrob. Agents Chemother. 49: 4061-4067 [Abstract] [Full Text]
Black, J. A., Moland, E. S., Thomson, K. S. (2005). AmpC Disk Test for Detection of Plasmid-Mediated AmpC {beta}-Lactamases in Enterobacteriaceae Lacking Chromosomal AmpC {beta}-Lactamases. J. Clin. Microbiol. 43: 3110-3113 [Abstract] [Full Text]
Li, W.-C., Huang, F.-Y., Liu, C.-P., Weng, L.-C., Wang, N.-Y., Chiu, N.-C., Chiang, C.-S. (2005). Ceftriaxone Resistance of Nontyphoidal Salmonella enterica Isolates in Northern Taiwan Attributable to Production of CTX-M-14 and CMY-2 {beta}-Lactamases. J. Clin. Microbiol. 43: 3237-3243 [Abstract] [Full Text]
Hasman, H., Mevius, D., Veldman, K., Olesen, I., Aarestrup, F. M. (2005). {beta}-Lactamases among extended-spectrum {beta}-lactamase (ESBL)-resistant Salmonella from poultry, poultry products and human patients in The Netherlands. J Antimicrob Chemother 56: 115-121 [Abstract] [Full Text]
Poppe, C., Martin, L. C., Gyles, C. L., Reid-Smith, R., Boerlin, P., McEwen, S. A., Prescott, J. F., Forward, K. R. (2005). Acquisition of Resistance to Extended-Spectrum Cephalosporins by Salmonella enterica subsp. enterica Serovar Newport and Escherichia coli in the Turkey Poult Intestinal Tract. Appl. Environ. Microbiol. 71: 1184-1192 [Abstract] [Full Text]
Mulvey, M. R., Bryce, E., Boyd, D. A., Ofner-Agostini, M., Land, A. M., Simor, A. E., Paton, S., the Canadian Hospital Epidemiology Committee, , the Canadian Nosocomial Infection Surveillance Pro, (2005). Molecular Characterization of Cefoxitin-Resistant Escherichia coli from Canadian Hospitals. Antimicrob. Agents Chemother. 49: 358-365 [Abstract] [Full Text]
Yan, J.-J., Wu, J.-J., Ko, W.-C., Tsai, S.-H., Chuang, C.-L., Wu, H.-M., Lu, Y.-J., Li, J.-D. (2004). Plasmid-mediated 16S rRNA methylases conferring high-level aminoglycoside resistance in Escherichia coli and Klebsiella pneumoniae isolates from two Taiwanese hospitals. J Antimicrob Chemother 54: 1007-1012 [Abstract] [Full Text]
Kruger, T., Szabo, D., Keddy, K. H., Deeley, K., Marsh, J. W., Hujer, A. M., Bonomo, R. A., Paterson, D. L. (2004). Infections with Nontyphoidal Salmonella Species Producing TEM-63 or a Novel TEM Enzyme, TEM-131, in South Africa. Antimicrob. Agents Chemother. 48: 4263-4270 [Abstract] [Full Text]
Kim, S., Kim, J., Kang, Y., Park, Y., Lee, B. (2004). Occurrence of Extended-Spectrum {beta}-Lactamases in Members of the Genus Shigella in the Republic of Korea. J. Clin. Microbiol. 42: 5264-5269 [Abstract] [Full Text]
Yan, J.-J., Ko, W.-C., Wu, H.-M., Tsai, S.-H., Chuang, C.-L., Wu, J.-J. (2004). Complexity of Klebsiella pneumoniae Isolates Resistant to Both Cephamycins and Extended-Spectrum Cephalosporins at a Teaching Hospital in Taiwan. J. Clin. Microbiol. 42: 5337-5340 [Abstract] [Full Text]
Giles, W. P., Benson, A. K., Olson, M. E., Hutkins, R. W., Whichard, J. M., Winokur, P. L., Fey, P. D. (2004). DNA Sequence Analysis of Regions Surrounding blaCMY-2 from Multiple Salmonella Plasmid Backbones. Antimicrob. Agents Chemother. 48: 2845-2852 [Abstract] [Full Text]
Gray, J. T., Hungerford, L. L., Fedorka-Cray, P. J., Headrick, M. L. (2004). Extended-Spectrum-Cephalosporin Resistance in Salmonella enterica Isolates of Animal Origin. Antimicrob. Agents Chemother. 48: 3179-3181 [Abstract] [Full Text]
Gebreyes, W. A., Thakur, S., Davies, P. R., Funk, J. A., Altier, C. (2004). Trends in antimicrobial resistance, phage types and integrons among Salmonella serotypes from pigs, 1997-2000. J Antimicrob Chemother 53: 997-1003 [Abstract] [Full Text]
Aarestrup, F. M., Hasman, H., Olsen, I., Sorensen, G. (2004). International Spread of blaCMY-2-Mediated Cephalosporin Resistance in a Multiresistant Salmonella enterica Serovar Heidelberg Isolate Stemming from the Importation of a Boar by Denmark from Canada. Antimicrob. Agents Chemother. 48: 1916-1917 [Full Text]
Yan, J.-J., Hong, C.-Y., Ko, W.-C., Chen, Y.-J., Tsai, S.-H., Chuang, C.-L., Wu, J.-J. (2004). Dissemination of blaCMY-2 among Escherichia coli Isolates from Food Animals, Retail Ground Meats, and Humans in Southern Taiwan. Antimicrob. Agents Chemother. 48: 1353-1356 [Abstract] [Full Text]
Zhao, S., Qaiyumi, S., Friedman, S., Singh, R., Foley, S. L., White, D. G., McDermott, P. F., Donkar, T., Bolin, C., Munro, S., Baron, E. J., Walker, R. D. (2003). Characterization of Salmonella enterica Serotype Newport Isolated from Humans and Food Animals. J. Clin. Microbiol. 41: 5366-5371 [Abstract] [Full Text]
Pitout, J. D. D., Reisbig, M. D., Mulvey, M., Chui, L., Louie, M., Crowe, L., Church, D. L., Elsayed, S., Gregson, D., Ahmed, R., Tilley, P., Hanson, N. D. (2003). Association between Handling of Pet Treats and Infection with Salmonella enterica Serotype Newport Expressing the AmpC {beta}-Lactamase, CMY-2. J. Clin. Microbiol. 41: 4578-4582 [Abstract] [Full Text]
Makanera, A., Arlet, G., Gautier, V., Manai, M. (2003). Molecular Epidemiology and Characterization of Plasmid-Encoded {beta}-Lactamases Produced by Tunisian Clinical Isolates of Salmonella enterica Serotype Mbandaka Resistant to Broad-Spectrum Cephalosporins. J. Clin. Microbiol. 41: 2940-2945 [Abstract] [Full Text]
Hanson, N. D. (2003). AmpC {beta}-lactamases: what do we need to know for the future?. J Antimicrob Chemother 52: 2-4 [Full Text]
Miriagou, V., Tzouvelekis, L. S., Rossiter, S., Tzelepi, E., Angulo, F. J., Whichard, J. M. (2003). Imipenem Resistance in a Salmonella Clinical Strain Due to Plasmid-Mediated Class A Carbapenemase KPC-2. Antimicrob. Agents Chemother. 47: 1297-1300 [Abstract] [Full Text]
Su, L.-H., Chiu, C.-H., Chu, C., Wang, M.-H., Chia, J.-H., Wu, T.-L. (2003). In Vivo Acquisition of Ceftriaxone Resistance in Salmonella enterica Serotype Anatum. Antimicrob. Agents Chemother. 47: 563-567 [Abstract] [Full Text]
Rankin, S. C., Aceto, H., Cassidy, J., Holt, J., Young, S., Love, B., Tewari, D., Munro, D. S., Benson, C. E. (2002). Molecular Characterization of Cephalosporin-Resistant Salmonella enterica Serotype Newport Isolates from Animals in Pennsylvania. J. Clin. Microbiol. 40: 4679-4684 [Abstract] [Full Text]
Miriagou, V., Filip, R., Coman, G., Tzouvelekis, L. S. (2002). Expanded-Spectrum Cephalosporin-Resistant Salmonella Strains in Romania. J. Clin. Microbiol. 40: 4334-4336 [Abstract] [Full Text]
Decre, D., Verdet, C., Raskine, L., Blanchard, H., Burghoffer, B., Philippon, A., Sanson-Le-Pors, M. J., Petit, J. C., Arlet, G. (2002). Characterization of CMY-type {beta}-lactamases in clinical strains of Proteus mirabilis and Klebsiella pneumoniae isolated in four hospitals in the Paris area. J Antimicrob Chemother 50: 681-688 [Abstract] [Full Text]
Gebreyes, W. A., Altier, C. (2002). Molecular Characterization of Multidrug-Resistant Salmonella enterica subsp. enterica Serovar Typhimurium Isolates from Swine. J. Clin. Microbiol. 40: 2813-2822 [Abstract] [Full Text]
Perez-Perez, F. J., Hanson, N. D. (2002). Detection of Plasmid-Mediated AmpC {beta}-Lactamase Genes in Clinical Isolates by Using Multiplex PCR. J. Clin. Microbiol. 40: 2153-2162 [Abstract] [Full Text]
Carattoli, A., Tosini, F., Giles, W. P., Rupp, M. E., Hinrichs, S. H., Angulo, F. J., Barrett, T. J., Fey, P. D. (2002). Characterization of Plasmids Carrying CMY-2 from Expanded-Spectrum Cephalosporin-Resistant Salmonella Strains Isolated in the United States between 1996 and 1998. Antimicrob. Agents Chemother. 46: 1269-1272 [Abstract] [Full Text]
Philippon, A., Arlet, G., Jacoby, G. A. (2002). Plasmid-Determined AmpC-Type {beta}-Lactamases. Antimicrob. Agents Chemother. 46: 1-11 [Full Text]
Zhao, S., White, D. G., McDermott, P. F., Friedman, S., English, L., Ayers, S., Meng, J., Maurer, J. J, Holland, R., Walker, R. D. (2001). Identification and Expression of Cephamycinase blaCMY Genes in Escherichia coli and Salmonella Isolates from Food Animals and Ground Meat. Antimicrob. Agents Chemother. 45: 3647-3650 [Abstract] [Full Text]
White, D. G., Zhao, S., Sudler, R., Ayers, S., Friedman, S., Chen, S., McDermott, P. F., McDermott, S., Wagner, D. D., Meng, J. (2001). The Isolation of Antibiotic-Resistant Salmonella from Retail Ground Meats. NEJM 345: 1147-1154 [Abstract] [Full Text]
Winokur, P. L., Vonstein, D. L., Hoffman, L. J., Uhlenhopp, E. K., Doern, G. V. (2001). Evidence for Transfer of CMY-2 AmpC {beta}-Lactamase Plasmids between Escherichia coli and Salmonella Isolates from Food Animals and Humans. Antimicrob. Agents Chemother. 45: 2716-2722 [Abstract] [Full Text]
Navarro, F., Perez-Trallero, E., Marimon, J. M., Aliaga, R., Gomariz, M., Mirelis, B. (2001). CMY-2-producing Salmonella enterica, Klebsiella pneumoniae, Klebsiella oxytoca, Proteus mirabilis and Escherichia coli strains isolated in Spain (October 1999-December 2000). J Antimicrob Chemother 48: 383-389 [Abstract] [Full Text]

Clin. Vaccine Immunol.	Clin. Microbiol. Rev.
J. Clin. Microbiol.	ALL ASM JOURNALS

Animal and Human Multidrug-Resistant, Cephalosporin-Resistant Salmonella Isolates Expressing a Plasmid-Mediated CMY-2 AmpC -Lactamase

Animal and Human Multidrug-Resistant, Cephalosporin-Resistant Salmonella Isolates Expressing a Plasmid-Mediated CMY-2 AmpC $beta$ -Lactamase