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Antimicrobial Agents and Chemotherapy, October 2000, p. 2845-2847, Vol. 44, No. 10
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The D-Alanine Residues of Staphylococcus aureus Teichoic Acids Alter the Susceptibility to Vancomycin and the Activity of Autolytic Enzymes

Andreas Peschel,* Cuong Vuong, Michael Otto, and Friedrich Götz

Microbial Genetics, University of Tübingen, 72076 Tübingen, Germany

Received 29 November 1999/Returned for modification 18 March 2000/Accepted 15 June 2000

Recently, Staphylococcus aureus strains with intermediate resistance to vancomycin, the antibiotic of last resort, have been described. Multiple changes in peptidoglycan turnover and structure contribute to the resistance phenotype. Here, we describe that structural changes of teichoic acids in the cell envelope have a considerable influence on the susceptibility to vancomycin and other glycopeptides. S. aureus cells lacking D-alanine esters in teichoic acids exhibited an at least threefold-increased sensitivity to glycopeptide antibiotics. Furthermore, the autolytic activity of the D-alanine mutant was reduced compared to the wild-type, and the mutant was more susceptible to the staphylolytic enzyme lysostaphin. Vancomycin inhibited autolysis at very high concentrations but neither in the wild-type nor in the mutant was the autolytic activity influenced in the range of the MIC. Mutant cells had a considerably higher capacity to bind vancomycin.

* Corresponding author. Mailing address: Microbial Genetics, University of Tübingen, Waldhäuser Str. 70/8, 72076 Tübingen, Germany. Phone: 49-7071-297-5938. Fax: 49-7071-29-5937. E-mail: andreas.peschel{at}uni-tuebingen.de.

Antimicrobial Agents and Chemotherapy, October 2000, p. 2845-2847, Vol. 44, No. 10
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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