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Antimicrobial Agents and Chemotherapy, October 2000, p. 2876-2879, Vol. 44, No. 10
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Detection of the High-Level Aminoglycoside Resistance Gene aph(2")-Ib in Enterococcus faecium

Susan J. Kao,1 Il You,1 Don B. Clewell,2 Susan M. Donabedian,3 Marcus J. Zervos,3 Joanne Petrin,4 Karen J. Shaw,4,dagger and Joseph W. Chow1,*

Research and Medical Service, John D. Dingell VA Medical Center, and Wayne State University School of Medicine, Detroit, Michigan 482011; Departments of Biologic and Materials Sciences and Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan 481092; William Beaumont Hospital, Royal Oak, Michigan 480733; and Schering-Plough Research Institute, Kenilworth, New Jersey 070334

Received 22 December 1999/Returned for modification 6 March 2000/Accepted 17 July 2000

A new high-level gentamicin resistance gene, designated aph(2")-Ib, was cloned from Enterococcus faecium SF11770. The deduced amino acid sequence of the 897-bp open reading frame of aph(2")-Ib shares homology with the aminoglycoside-modifying enzymes AAC(6')-APH(2"), APH(2")-Ic, and APH(2")-Id. The observed phosphotransferase activity is designated APH(2")-Ib.

* Corresponding author. Mailing address: Medical Service, John D. Dingell VA Medical Center, Detroit, MI 48201-1932. Phone: (313) 576-3310. Fax: (313) 576-1122. E-mail: aa2563{at}wayne.edu.

dagger Present address: RW Johnson Pharmaceutical Research Institute, San Diego, CA 92121.

Antimicrobial Agents and Chemotherapy, October 2000, p. 2876-2879, Vol. 44, No. 10
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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