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Antimicrobial Agents and Chemotherapy, October 2000, p. 2911-2914, Vol. 44, No. 10
Laboratory of Bacteriology and Medical
Mycology1 and Laboratory of Cellular
Biology,2 Istituto Superiore di Sanità,
Dipartimento di Biologia, Università di Roma
Tre,3 and IRCCS Lazzaro
Spallanzani,4 Rome, Italy
Received 17 February 2000/Returned for modification 18 May
2000/Accepted 1 July 2000
A conjugative IncL/M plasmid (pSEM) conferring resistance to
gentamicin, amikacin, kanamycin, sulfonamides, and expanded-spectrum cephalosporins was found in pathogenic strains of Salmonella
enterica serotype Typhimurium. Resistance to aminoglycosides was
encoded by a sul1-type class 1 integron (In-t3). An
extended-spectrum beta-lactamase gene,
blaSHV-5, was identified 3.5 kb downstream of
the integrase (intI1) gene of In-t3. Nucleotide sequence
analysis of the 5.3-kb blaSHV-5-In-t3 region
of pSEM highlighted striking similarities with IncL/M plasmids isolated
from nosocomial gram-negative pathogens, conferring resistance to
expanded-spectrum cephalosporins and aminoglycosides.
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Multiple-Antibiotic Resistance Mediated by Structurally
Related IncL/M Plasmids Carrying an Extended-Spectrum
-Lactamase Gene and a Class 1 Integron
*
Corresponding author. Mailing address: Laboratory of
Bacteriology and Medical Mycology, Istituto Superiore di Sanità,
viale Regina Elena 299, 00161 Rome, Italy. Phone: 39-06-4990-3128. Fax: 39-06-4938-7112. E-mail: alecara{at}iss.it.
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