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Antimicrobial Agents and Chemotherapy, October 2000, p. 2911-2914, Vol. 44, No. 10
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Multiple-Antibiotic Resistance Mediated by Structurally Related IncL/M Plasmids Carrying an Extended-Spectrum beta -Lactamase Gene and a Class 1 Integron

Laura Villa,1 Cristina Pezzella,1 Fabio Tosini,2 Paolo Visca,3,4 Andrea Petrucca,1 and Alessandra Carattoli1,*

Laboratory of Bacteriology and Medical Mycology1 and Laboratory of Cellular Biology,2 Istituto Superiore di Sanità, Dipartimento di Biologia, Università di Roma Tre,3 and IRCCS Lazzaro Spallanzani,4 Rome, Italy

Received 17 February 2000/Returned for modification 18 May 2000/Accepted 1 July 2000

A conjugative IncL/M plasmid (pSEM) conferring resistance to gentamicin, amikacin, kanamycin, sulfonamides, and expanded-spectrum cephalosporins was found in pathogenic strains of Salmonella enterica serotype Typhimurium. Resistance to aminoglycosides was encoded by a sul1-type class 1 integron (In-t3). An extended-spectrum beta-lactamase gene, blaSHV-5, was identified 3.5 kb downstream of the integrase (intI1) gene of In-t3. Nucleotide sequence analysis of the 5.3-kb blaSHV-5-In-t3 region of pSEM highlighted striking similarities with IncL/M plasmids isolated from nosocomial gram-negative pathogens, conferring resistance to expanded-spectrum cephalosporins and aminoglycosides.

* Corresponding author. Mailing address: Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, viale Regina Elena 299, 00161 Rome, Italy. Phone: 39-06-4990-3128. Fax: 39-06-4938-7112. E-mail: alecara{at}iss.it.

Antimicrobial Agents and Chemotherapy, October 2000, p. 2911-2914, Vol. 44, No. 10
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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