This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ASM journals
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bemer-Melchior, P.
Right arrow Articles by Drugeon, H.-B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bemer-Melchior, P.
Right arrow Articles by Drugeon, H.-B.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, November 2000, p. 2999-3002, Vol. 44, No. 11
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

In Vitro Activity of the New Ketolide Telithromycin Compared with Those of Macrolides against Streptococcus pyogenes: Influences of Resistance Mechanisms and Methodological Factors

Pascale Bemer-Melchior,1,* Marie-Emmanuelle Juvin,1 Sandrine Tassin,1 Andre Bryskier,2 Gian Carlo Schito,3 and Henri-B. Drugeon1

Department of Microbiology, Laënnec University Hospital, Nantes,1 and Clinical Pharmacology of Anti-Infectives, Hoechst Marion Roussel, Romainville,2 France, Institute of Microbiology, University of Genoa, Genoa, Italy3

Received 19 May 2000/Returned for modification 22 June 2000/Accepted 2 August 2000

One hundred and seven clinical isolates of Streptococcus pyogenes, 80 susceptible to macrolides and 27 resistant to erythromycin A (MIC >0.5 µg/ml), were examined. The erythromycin A-lincomycin double-disk test assigned 7 resistant strains to the M-phenotype, 8 to the inducible macrolide, lincosamide, and streptogramin B resistance (iMLSB) phenotype, and 12 to the constitutive MLSB resistance (cMLSB) phenotype. MICs of erythromycin A, clarithromycin, azithromycin, roxithromycin, and clindamycin were determined by a broth microdilution method. MICs of telithromycin were determined by three different methods (broth microdilution, agar dilution, and E-test methods) in an ambient air atmosphere and in a 5 to 6% CO2 atmosphere. Erythromycin A resistance genes were investigated by PCR in the 27 erythromycin A-resistant isolates. MICs of erythromycin A and clindamycin showed six groups of resistant strains, groups A to F. iMLSB strains (A, B, and D groups) are characterized by two distinct patterns of resistance correlated with genotypic results. A- and B-group strains were moderately resistant to 14- and 15-membered ring macrolides and highly susceptible to telithromycin. All A- and B-group isolates harbored erm TR gene, D-group strains, highly resistant to macrolides and intermediately resistant to telithromycin (MICs, 1 to 16 µg/ml), were all characterized by having the ermB gene. All M-phenotype isolates (C group), resistant to 14- and 15-membered ring macrolides and susceptible to clindamycin and telithromycin, harbored the mefA gene. All cMLSB strains (E and F groups) with high level of resistance to macrolides, lincosamide, and telithromycin had the ermB gene. The effect of 5 to 6% CO2 was remarkable on resistant strains, by increasing MICs of telithromycin from 1 to 6 twofold dilutions against D-E- and F-group isolates.

* Corresponding author. Mailing address: Department of Microbiology, Laënnec University Hospital, Bd J. Monod, 44093 Saint-Herblain Cedex 1, France. Phone: (33) 2 40 16 54 57. Fax: (33) 2 40 16 54 55. E-mail: pascale.bemermelchior{at}chu-nantes.fr.

Antimicrobial Agents and Chemotherapy, November 2000, p. 2999-3002, Vol. 44, No. 11
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Richter, S. S., Heilmann, K. P., Dohrn, C. L., Beekmann, S. E., Riahi, F., Garcia-de-Lomas, J., Ferech, M., Goossens, H., Doern, G. V. (2008). Increasing telithromycin resistance among Streptococcus pyogenes in Europe. J Antimicrob Chemother 61: 603-611 [Abstract] [Full Text]  
  • Jing, H.-b., Ning, B.-a., Hao, H.-j., Zheng, Y.-l., Chang, D., Jiang, W., Jiang, Y.-q. (2006). Epidemiological analysis of group A streptococci recovered from patients in China.. J Med Microbiol 55: 1101-1107 [Abstract] [Full Text]  
  • Rantala, M., Haanpera-Heikkinen, M., Lindgren, M., Seppala, H., Huovinen, P., Jalava, J., the Finnish Study Group for Antimicrobial Resistan, (2006). Streptococcus pneumoniae Isolates Resistant to Telithromycin. Antimicrob. Agents Chemother. 50: 1855-1858 [Abstract] [Full Text]  
  • Bouchillon, S. K., Johnson, J. L., Hoban, D. J., Stevens, T. M., Johnson, B. M. (2005). Impact of carbon dioxide on the susceptibility of key respiratory tract pathogens to telithromycin and azithromycin. J Antimicrob Chemother 56: 224-227 [Abstract] [Full Text]  
  • Klaassen, C. H. W., Mouton, J. W. (2005). Molecular Detection of the Macrolide Efflux Gene: To Discriminate or Not To Discriminate between mef(A) and mef(E). Antimicrob. Agents Chemother. 49: 1271-1278 [Full Text]  
  • Canton, R., Mazzariol, A., Morosini, M.-I., Baquero, F., Cornaglia, G. (2005). Telithromycin activity is reduced by efflux in Streptococcus pyogenes. J Antimicrob Chemother 55: 489-495 [Abstract] [Full Text]  
  • Batard, E., Juvin, M. E., Jacqueline, C., Bugnon, D., Caillon, J., Potel, G., Drugeon, H. B. (2005). Influence of Carbon Dioxide on the MIC of Telithromycin for Streptococcus pneumoniae: an In Vitro-In Vivo Study. Antimicrob. Agents Chemother. 49: 464-466 [Abstract] [Full Text]  
  • Reinert, R. R. (2004). Clinical efficacy of ketolides in the treatment of respiratory tract infections. J Antimicrob Chemother 53: 918-927 [Abstract] [Full Text]  
  • Walsh, F., Carnegy, F., Willcock, J., Amyes, S. (2004). Comparative in vitro activity of telithromycin against macrolide-resistant and -susceptible Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae. J Antimicrob Chemother 53: 793-796 [Abstract] [Full Text]  
  • Hsueh, P.-R., Teng, L.-J., Lee, C.-M., Huang, W.-K., Wu, T.-L., Wan, J.-H., Yang, D., Shyr, J.-M., Chuang, Y.-C., Yan, J.-J., Lu, J.-J., Wu, J.-J., Ko, W.-C., Chang, F.-Y., Yang, Y.-C., Lau, Y.-J., Liu, Y.-C., Leu, H.-S., Liu, C.-Y., Luh, K.-T. (2003). Telithromycin and Quinupristin-Dalfopristin Resistance in Clinical Isolates of Streptococcus pyogenes: SMART Program 2001 Data. Antimicrob. Agents Chemother. 47: 2152-2157 [Abstract] [Full Text]  
  • Morosini, M.-I., Canton, R., Loza, E., del Campo, R., Almaraz, F., Baquero, F. (2003). Streptococcus pyogenes isolates with characterized macrolide resistance mechanisms in Spain: in vitro activities of telithromycin and cethromycin. J Antimicrob Chemother 52: 50-55 [Abstract] [Full Text]  
  • Ackermann, G., Rodloff, A. C. (2003). Drugs of the 21st century: telithromycin (HMR 3647)--the first ketolide. J Antimicrob Chemother 51: 497-511 [Abstract] [Full Text]  
  • Reinert, R. R., Lutticken, R., Bryskier, A., Al-Lahham, A. (2003). Macrolide-Resistant Streptococcus pneumoniae and Streptococcus pyogenes in the Pediatric Population in Germany during 2000-2001. Antimicrob. Agents Chemother. 47: 489-493 [Abstract] [Full Text]  
  • Betriu, C., Culebras, E., Redondo, M., Rodriguez-Avial, I., Gomez, M., Boloix, A., Picazo, J. J. (2002). Prevalence of macrolide and tetracycline resistance mechanisms in Streptococcus pyogenes isolates and in vitro susceptibility to telithromycin. J Antimicrob Chemother 50: 436-438 [Full Text]  
  • Hakanen, A., Huovinen, P., Kotilainen, P., Siitonen, A., Jousimies-Somer, H. (2002). Quality Control Strains Used in Susceptibility Testing of Campylobacter spp.. J. Clin. Microbiol. 40: 2705-2706 [Full Text]  
  • Nagai, K., Appelbaum, P. C., Davies, T. A., Kelly, L. M., Hoellman, D. B., Andrasevic, A. T., Drukalska, L., Hryniewicz, W., Jacobs, M. R., Kolman, J., Miciuleviciene, J., Pana, M., Setchanova, L., Thege, M. K., Hupkova, H., Trupl, J., Urbaskova, P. (2002). Susceptibilities to Telithromycin and Six Other Agents and Prevalence of Macrolide Resistance Due to L4 Ribosomal Protein Mutation among 992 Pneumococci from 10 Central and Eastern European Countries. Antimicrob. Agents Chemother. 46: 371-377 [Abstract] [Full Text]  
  • Nagai, K., Appelbaum, P. C., Davies, T. A., Kelly, L. M., Hoellman, D. B., Andrasevic, A. T., Drukalska, L., Hryniewicz, W., Jacobs, M. R., Kolman, J., Miciuleviciene, J., Pana, M., Setchanova, L., Thege, M. K., Hupkova, H., Trupl, J., Urbaskova, P. (2002). Susceptibility to Telithromycin in 1,011 Streptococcus pyogenes Isolates from 10 Central and Eastern European Countries. Antimicrob. Agents Chemother. 46: 546-549 [Abstract] [Full Text]  
  • Nagai, K., Davies, T. A., Ednie, L. M., Bryskier, A., Palavecino, E., Jacobs, M. R., Appelbaum, P. C. (2001). Activities of a New Fluoroketolide, HMR 3787, and Its (Des)-Fluor Derivative RU 64399 Compared to Those of Telithromycin, Erythromycin A, Azithromycin, Clarithromycin, and Clindamycin against Macrolide-Susceptible or -Resistant Streptococcus pneumoniae and S. pyogenes. Antimicrob. Agents Chemother. 45: 3242-3245 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»