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Antimicrobial Agents and Chemotherapy, November 2000, p. 3133-3136, Vol. 44, No. 11
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Antibiotic Susceptibility Pattern of Mycobacterium marinum

Alexandra Aubry,1 Vincent Jarlier,1 Sylvie Escolano,2 Chantal Truffot-Pernot,1 and Emmanuelle Cambau1,*

Laboratoire de Bactériologie-Hygiène et Centre National de Référence pour la Surveillance des Infections à Mycobactéries et de leur Résistance aux Antituberculeux,1 and Unité INSERM U436,2 Faculté de Médecine Pitié-Salpêtrière, Paris, France

Received 18 May 2000/Returned for modification 20 July 2000/Accepted 24 August 2000

In vitro activities of 17 antibiotics against 53 clinical strains of Mycobacterium marinum, an atypical mycobacterium responsible for cutaneous infections, were determined using the reference agar dilution method. Rifampin and rifabutin were the most active drugs (MICs at which 90% of the isolates tested were inhibited [MIC90s], 0.5 and 0.6 µg/ml, respectively). MICs of minocycline (MIC90, 4 µg/ml), doxycycline (MIC90, 16 µg/ml), clarithromycin (MIC90, 4 µg/ml), sparfloxacin (MIC90, 2 µg/ml), moxifloxacin (MIC90, 1 µg/ml), imipenem (MIC90, 8 µg/ml), sulfamethoxazole (MIC90, 8 µg/ml) and amikacin (MIC90, 4 µg/ml) were close to the susceptibility breakpoints. MICs of isoniazid, ethambutol, trimethoprim, azithromycin, ciprofloxacin, ofloxacin, and levofloxacin were above the concentrations usually obtained in vivo. For each drug, the MIC50, geometric mean MIC, and modal MIC were very close, showing that all the strains had a similar susceptibility pattern. Percent agreement (within ±1 log2 dilution) between MICs yielded by the Etest method and by the agar dilution method used as reference were 83, 59, 43, and 24% for minocycline, rifampin, clarithromycin, and sparfloxacin, respectively. Reproducibility with the Etest was low, in contrast to that with the agar dilution method. In conclusion, M. marinum is a naturally multidrug-resistant species for which the agar dilution method is more accurate than the Etest for antibiotic susceptibility testing.

* Corresponding author. Mailing address: Faculté de Médecine Pitié-Salpêtrière, 91, Boulevard de l'Hôpital, 75634 Paris Cedex 13, France. Phone: (33) 1 40 77 97 46. Fax: (33) 1 45 82 75 77. E-mail: cambau{at}chups.jussieu.fr.

Antimicrobial Agents and Chemotherapy, November 2000, p. 3133-3136, Vol. 44, No. 11
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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