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Antimicrobial Agents and Chemotherapy, November 2000, p. 3158-3162, Vol. 44, No. 11
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Nucleotide Sequence of the Chromosomal ampC Gene of Enterobacter aerogenes

Karen E. Preston,1,* Christopher C. A. Radomski,2 and Richard A. Venezia1

Department of Pathology and Laboratory Medicine, Albany Medical Center Hospital, Albany, New York 12208,1 and TerraGen Discovery, Inc., Vancouver, British Columbia V6T 1Z3, Canada2

Received 18 January 2000/Returned for modification 18 May 2000/Accepted 2 August 2000

The AmpC beta -lactamase gene and a small portion of the regulatory ampR sequence of Enterobacter aerogenes 97B were cloned and sequenced. The beta -lactamase had an isoelectric point of 8 and conferred cephalosporin and cephamycin resistance on the host. The sequence of the cloned gene is most closely related to those of the ampC genes of E. cloacae and C. freundii.

* Corresponding author. Mailing address: Department of Pathology and Laboratory Medicine, Mail Code 22, Albany Medical Center Hospital, 43 New Scotland Ave., Albany, NY 12208. Phone: (518) 262-4270. Fax: (518) 262-4337. E-mail: PrestoK{at}mail.amc.edu.

Antimicrobial Agents and Chemotherapy, November 2000, p. 3158-3162, Vol. 44, No. 11
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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