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Antimicrobial Agents and Chemotherapy, November 2000, p. 3196-3198, Vol. 44, No. 11
0066-4804/00/$04.00+0

Resistance to Multiple Fluoroquinolones in a Clinical Isolate of Streptococcus pyogenes: Identification of gyrA and parC and Specification of Point Mutations Associated with Resistance

S. Steve Yan,1 Melissa L. Fox,2 Steven M. Holland,2 Frida Stock,1 Vee J. Gill,1 and Daniel P. Fedorko1,*

Microbiology Service, CPD, Clinical Center,1 and the Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases,2 National Institutes of Health, Bethesda, Maryland 20892-1508

Received 3 March 2000/Returned for modification 9 June 2000/Accepted 17 August 2000

A strain of Streptococcus pyogenes resistant to multiple fluoroquinolones was isolated from the blood of an immunocompromised patient. Resistance to fluoroquinolones in S. pyogenes has not been previously studied. Compared to 10 sensitive strains of S. pyogenes, the fluoroquinolone-resistant clinical isolate of S. pyogenes presented point mutations in gyrA, predicting that serine-81 was changed to phenylalanine and that methionine-99 was changed to leucine, and in parC, predicting that serine-79 was changed to tyrosine. The mechanism of fluoroquinolone resistance in this isolate of S. pyogenes appears to be analogous to previously reported mechanisms for Streptococcus pneumoniae.

* Corresponding author. Mailing address: Microbiology Service, CPD, Clinical Center, National Institutes of Health, Building 10, Rm. 2C385, Bethesda, MD 20892-1508. Phone: (301) 496-4433. Fax: (301) 402-1886. E-mail: dfedorko{at}mail.cc.nih.gov.

Antimicrobial Agents and Chemotherapy, November 2000, p. 3196-3198, Vol. 44, No. 11
0066-4804/00/$04.00+0


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