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Antimicrobial Agents and Chemotherapy, December 2000, p. 3425-3427, Vol. 44, No. 12
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Presence of Variations in Ribosomal Protein L16 Corresponding to Susceptibility of Enterococci to Oligosaccharides (Avilamycin and Evernimicin)

Frank Møller Aarestrup* and Lars Bogø Jensen

Danish Veterinary Laboratory, DK-1790 Copenhagen V, Denmark

Received 30 November 1999/Returned for modification 28 July 2000/Accepted 24 August 2000

Fragments (414 bp) of the gene-encoding ribosomal protein L16 from Enterococcus faecium and Enterococcus faecalis that were resistant and susceptible to the oligosaccharide antibiotics avilamycin and evernimicin (SCH 27899) were sequenced and compared. The susceptible E. faecalis and E. faecium isolates had sequences that were similar to those of the type strains. All resistant E. faecalis isolates contained the same base pair variation [CGT (Arg-56) right-arrow CAT (His-56)]. The same variation and two additional variations [ATC (Ile-52) right-arrow ACC (Thr-52) and ATC (Ile-52) right-arrow AGC (Ser-52)] were found in the resistant E. faecium isolates. This study indicated that resistance to the oligosaccharides in enterococci is associated with variations in the ribosomal protein L16.

* Corresponding author. Mailing address: Danish Veterinary Laboratory, 27 Bülowsvej, DK-1790 Copenhagen V, Denmark. Phone: 45 35 30 01 00. Fax: 45 35 30 01 20. E-mail: faa{at}svs.dk.

Antimicrobial Agents and Chemotherapy, December 2000, p. 3425-3427, Vol. 44, No. 12
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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