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Antimicrobial Agents and Chemotherapy, December 2000, p. 3461-3464, Vol. 44, No. 12
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Use of Molecular Beacons To Detect an Antifolate Resistance-Associated Mutation in Plasmodium falciparum

Rémy Durand,* Jobin Eslahpazire, Sayeh Jafari, Jean-François Delabre, Anne Marmorat-Khuong, Jean-Philippe di Piazza, and Jacques Le Bras

Centre National de Référence pour la Chimiosensibilité du Paludisme, Université Paris V et Assistance Publique-Hôpitaux de Paris, Hôpital Bichat-Claude Bernard, Laboratoire de Parasitologie, 75877 Paris Cedex 18, France

Received 25 August 1999/Returned for modification 3 November 1999/Accepted 11 September 2000

A PCR-based technique using new fluorescent probes, called molecular beacons, was developed to detect the antifolate resistance-associated S108N point mutation in Plasmodium falciparum. One hundred African clinical isolates were tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This new molecular technique appears to be a promising tool for epidemiological studies.

* Corresponding author. Mailing address: Laboratoire de Parasitologie, Hôpital Bichat-Claude Bernard, 46 rue Henri Huchard, 78877 Paris Cedex 18, France. Phone: 33 1 40 25 78 81. Fax: 33 1 40 25 67 63. E-mail: rjdurand{at}club-internet.fr.

Antimicrobial Agents and Chemotherapy, December 2000, p. 3461-3464, Vol. 44, No. 12
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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