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Antimicrobial Agents and Chemotherapy, February 2000, p. 428-432, Vol. 44, No. 2
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cloning, Nucleotide Sequencing, and Analysis of the Gene Encoding an AmpC beta -Lactamase in Acinetobacter baumannii

Germán Bou* and Jesús Martínez-Beltrán

Servicio de Microbiología, Hospital Ramón y Cajal, 28034 Madrid, Spain

Received 13 January 1999/Returned for modification 29 August 1999/Accepted 5 November 1999

A clinical strain of Acinetobacter baumannii (strain Ab RYC 52763/97) that was isolated during an outbreak in our hospital and that was resistant to all beta -lactam antibiotics tested produced three beta -lactamases: a TEM-1-type (pI, 5.4) plasmid-mediated beta -lactamase, a chromosomally mediated OXA-derived (pI, 9.0) beta -lactamase, and a presumptive chromosomal cephalosporinase (pI, 9.4). The nucleotide sequence of the chromosomal cephalosporinase gene shows for the first time the gene encoding an AmpC beta -lactamase in A. baumannii. In addition, we report here the biochemical properties of this A. baumannii AmpC beta -lactamase.

* Corresponding author. Present address: Department of Immunology and Division of Infectious Diseases, Mayo Clinic, 200 First St. SW, Guggenheim 516, Rochester, MN 55905. Phone: (507) 284-9504. Fax: (507) 284-3757. E-mail: germanbou{at}mailcity.com.

Antimicrobial Agents and Chemotherapy, February 2000, p. 428-432, Vol. 44, No. 2
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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