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Antimicrobial Agents and Chemotherapy, February 2000, p. 437-440, Vol. 44, No. 2
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification and Characterization of the Point Mutation Which Affects the Transcription Level of the Chromosomal 3-N-Acetyltransferase Gene of Streptomyces griseus SS-1198

Jun Ishikawa,* Atsuko Sunada, Ritsuko Oyama, and Kunimoto Hotta

Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162-8640, Japan

Received 9 April 1999/Returned for modification 18 July 1999/Accepted 4 October 1999

We determined the molecular basis for the enhanced expression of the aac(3)-Xa gene encoding an aminoglycoside 3-N-acetyltransferase in Streptomyces griseus. A Cright-arrowT substitution was identified at the putative promoter of the mutant gene. RNA analyses demonstrated that the substitution caused a marked increase in the production of the gene-specific transcripts. Therefore, it seemed very likely that the aac(3)-Xa gene was activated by the substitution resulting in the emergence of a stronger promoter.

* Corresponding author. Mailing address: Department of Bioactive Molecules, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan. Phone: 81 3 (5285) 1111. Fax: 81 3 (5285) 1272. E-mail: jun{at}nih.go.jp.

Antimicrobial Agents and Chemotherapy, February 2000, p. 437-440, Vol. 44, No. 2
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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