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Antimicrobial Agents and Chemotherapy, March 2000, p. 787-789, Vol. 44, No. 3
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Streptococcus gordonii Strains Resistant to Fluorodeoxyuridine Contain Mutations in the Thymidine Kinase Gene and Are Deficient in Thymidine Kinase Activity

C. A. Franke,1,2 T. M. Bolman,1 S. A. Ottum,2 K. F. Jones,1 and D. E. Hruby1,2,*

SIGA Pharmaceuticals, Inc.,1 and Department of Microbiology and Center for Gene Research and Biotechnology, Oregon State University,2 Corvallis, Oregon

Received 2 September 1999/Returned for modification 11 November 1999/Accepted 3 December 1999

Mutants of Streptococcus gordonii resistant to 5-fluorodeoxyuridine (FUdRr) were isolated. Each strain contained a point mutation resulting in the premature termination of the thymidine kinase (TK) open reading frame (tdk). In vitro translation of the mutant tdk coding regions resulted in synthesis of truncated TK polypeptides deficient in TK activity.

* Corresponding author. Mailing address: Siga Pharmaceuticals, Inc., 4575 SW Research Way, Suite 230, Corvallis, OR 97333. Phone: (541) 753-2000. Fax: (541) 753-9999. E-mail: dhruby{at}sgph.com.

Antimicrobial Agents and Chemotherapy, March 2000, p. 787-789, Vol. 44, No. 3
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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