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Antimicrobial Agents and Chemotherapy, March 2000, p. 798-801, Vol. 44, No. 3
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Convenient Assay for Estimating the Possible Involvement of Efflux of Fluoroquinolones by Streptococcus pneumoniae and Staphylococcus aureus: Evidence for Diminished Moxifloxacin, Sparfloxacin, and Trovafloxacin Efflux

Rebecca Beyer,1 Ekaterina Pestova,1 John J. Millichap,1 Valentina Stosor,1,2 Gary A. Noskin,1,2 and Lance R. Peterson1,2,*

Department of Pathology, Division of Microbiology,1 and Department of Medicine, Division of Infectious Diseases,2 Northwestern University Medical School, Chicago, Illinois 60611

Received 16 August 1999/Returned for modification 28 September 1999/Accepted 6 December 1999

We developed a simplified assay for estimating efflux by measuring the effect of reserpine on the growth of Streptococcus pneumoniae and Staphylococcus aureus over 7 h. Reserpine enhanced ciprofloxacin and levofloxacin 17 to 68%. The hydrophobic drug trovafloxacin and the drug moxifloxacin, with a bulky C-7 substituent but hydrophilicity similar to that of levofloxacin, showed little (0 to 11%) reserpine-enhancing effect. The ease of resistant mutant strain selection correlated with efflux susceptibility.

* Corresponding author. Mailing address: Microbiology Division, Department of Pathology, Wesley Pavilion, Room 565, Northwestern Memorial Hospital, 251 E. Huron, Chicago, IL 60611. Phone: (312) 926-2885. Fax: (312) 926-4139. E-mail: lancer{at}nwu.edu.

Antimicrobial Agents and Chemotherapy, March 2000, p. 798-801, Vol. 44, No. 3
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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