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Antimicrobial Agents and Chemotherapy, April 2000, p. 1075-1077, Vol. 44, No. 4
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Mutations in the Beginning of the rpoB Gene Can Induce Resistance to Rifamycins in both Helicobacter pylori and Mycobacterium tuberculosis

Markus Heep,* Ulrich Rieger, Daniela Beck, and Norbert Lehn

Institute for Medical Microbiology, Regensburg, Germany

Received 26 July 1999/Returned for modification 23 September 1999/Accepted 10 January 2000

A clinical isolate of Helicobacter pylori that developed resistance to rifabutin during therapy carried an rpoB gene that retained a wild-type cluster region sequence but had acquired a novel codon 149 (V149F) mutation. In transformation experiments, the mutation was shown to confer high-level rifabutin resistance. The equivalent mutation (V176F) was present in several resistant isolates of Mycobacterium tuberculosis.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie, Uniklinik Regensburg, D-93053, Regensburg, Germany. Phone: 49 941 944-6414. Fax: 49 941 944-6402. E-mail: Markus.Heep{at}klinik.uni-regensburg.de.

Antimicrobial Agents and Chemotherapy, April 2000, p. 1075-1077, Vol. 44, No. 4
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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