This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Poirel, L.
Right arrow Articles by Nordmann, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Poirel, L.
Right arrow Articles by Nordmann, P.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, April 2000, p. 891-897, Vol. 44, No. 4
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of VIM-2, a Carbapenem-Hydrolyzing Metallo-beta -Lactamase and Its Plasmid- and Integron-Borne Gene from a Pseudomonas aeruginosa Clinical Isolate in France

Laurent Poirel,1 Thierry Naas,1 Delphine Nicolas,1 Louis Collet,2 Samuel Bellais,1 Jean-Didier Cavallo,3 and Patrice Nordmann1,*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre,1 Laboratoire de Biologie, Institut Paoli-Calmettes, 13273 Marseille,2 and Laboratoire de Biologie Médicale, Hôpital d'Instruction des Armées Bégin, 94160 Saint Mandé,3 France

Received 13 October 1999/Returned for modification 28 December 1999/Accepted 18 January 2000

Pseudomonas aeruginosa COL-1 was identified in a blood culture of a 39-year-old-woman treated with imipenem in Marseilles, France, in 1996. This strain was resistant to beta -lactams, including ureidopenicillins, ticarcillin-clavulanic acid, cefepime, ceftazidime, imipenem, and meropenem, but remained susceptible to the monobactam aztreonam. The carbapenem-hydrolyzing beta -lactamase gene of P. aeruginosa COL-1 was cloned, sequenced, and expressed in Escherichia coli DH10B. The deduced 266-amino-acid protein was an Ambler class B beta -lactamase, with amino acid identities of 32% with B-II from Bacillus cereus; 31% with IMP-1 from several gram-negative rods in Japan, including P. aeruginosa; 27% with CcrA from Bacteroides fragilis; 24% with BlaB from Chryseobacterium meningosepticum; 24% with IND-1 from Chryseobacterium indologenes; 21% with CphA-1 from Aeromonas hydrophila; and 11% with L-1 from Stenotrophomonas maltophilia. It was most closely related to VIM-1 beta -lactamase recently reported from Italian P. aeruginosa clinical isolates (90% amino acid identity). Purified VIM-2 beta -lactamase had a pI of 5.6, a relative molecular mass of 29.7 kDa, and a broad substrate hydrolysis range, including penicillins, cephalosporins, cephamycins, oxacephamycins, and carbapenems, but not monobactams. As a metallo-beta -lactamase, its activity was zinc dependent and inhibited by EDTA (50% inhibitory concentration, 50 µM). VIM-2 conferred a resistance pattern to beta -lactams in E. coli DH10B that paralleled its in vitro hydrolytic properties, except for susceptibility to ureidopenicillins, carbapenems, and cefepime. blaVIM-2 was located on a ca. 45-kb plasmid that in addition conferred resistance to sulfamides and that was not self-transmissible either from P. aeruginosa to E. coli or from E. coli to E. coli. blaVIM-2 was the only gene cassette located within the variable region of a novel class 1 integron, In56, that was weakly related to the blaVIM-1-containing integron. VIM-2 is the second carbapenem-hydrolyzing metalloenzyme characterized from a P. aeruginosa isolate outside Japan.


* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre cedex, France. Phone: 33 1 45 21 36 32. Fax: 33 1 45 21 63 40. E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr.


Antimicrobial Agents and Chemotherapy, April 2000, p. 891-897, Vol. 44, No. 4
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Yong, D., Toleman, M. A., Giske, C. G., Cho, H. S., Sundman, K., Lee, K., Walsh, T. R. (2009). Characterization of a New Metallo-{beta}-Lactamase Gene, blaNDM-1, and a Novel Erythromycin Esterase Gene Carried on a Unique Genetic Structure in Klebsiella pneumoniae Sequence Type 14 from India. Antimicrob. Agents Chemother. 53: 5046-5054 [Abstract] [Full Text]  
  • Rodriguez-Martinez, J.-M., Poirel, L., Nordmann, P. (2009). Molecular Epidemiology and Mechanisms of Carbapenem Resistance in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 53: 4783-4788 [Abstract] [Full Text]  
  • Picao, R. C., Poirel, L., Gales, A. C., Nordmann, P. (2009). Diversity of {beta}-Lactamases Produced by Ceftazidime-Resistant Pseudomonas aeruginosa Isolates Causing Bloodstream Infections in Brazil. Antimicrob. Agents Chemother. 53: 3908-3913 [Abstract] [Full Text]  
  • Strateva, T., Yordanov, D. (2009). Pseudomonas aeruginosa - a phenomenon of bacterial resistance. J Med Microbiol 58: 1133-1148 [Abstract] [Full Text]  
  • Zhao, W.-H., Chen, G., Ito, R., Hu, Z.-Q. (2009). Relevance of resistance levels to carbapenems and integron-borne blaIMP-1, blaIMP-7, blaIMP-10 and blaVIM-2 in clinical isolates of Pseudomonas aeruginosa. J Med Microbiol 58: 1080-1085 [Abstract] [Full Text]  
  • Souli, M., Rekatsina, P. D., Chryssouli, Z., Galani, I., Giamarellou, H., Kanellakopoulou, K. (2009). Does the Activity of the Combination of Imipenem and Colistin In Vitro Exceed the Problem of Resistance in Metallo-{beta}-Lactamase-Producing Klebsiella pneumoniae Isolates?. Antimicrob. Agents Chemother. 53: 2133-2135 [Abstract] [Full Text]  
  • Siarkou, V. I., Vitti, D., Protonotariou, E., Ikonomidis, A., Sofianou, D. (2009). Molecular Epidemiology of Outbreak-Related Pseudomonas aeruginosa Strains Carrying the Novel Variant blaVIM-17 Metallo-{beta}-Lactamase Gene. Antimicrob. Agents Chemother. 53: 1325-1330 [Abstract] [Full Text]  
  • Qu, T.-t., Zhang, J.-l., Wang, J., Tao, J., Yu, Y.-s., Chen, Y.-g., Zhou, J.-y., Li, L.-j. (2009). Evaluation of Phenotypic Tests for Detection of Metallo-{beta}-Lactamase-Producing Pseudomonas aeruginosa Strains in China. J. Clin. Microbiol. 47: 1136-1142 [Abstract] [Full Text]  
  • Uma Karthika, R., Srinivasa Rao, R., Sahoo, S., Shashikala, P., Kanungo, R., Jayachandran, S., Prashanth, K. (2009). Phenotypic and genotypic assays for detecting the prevalence of metallo-{beta}-lactamases in clinical isolates of Acinetobacter baumannii from a South Indian tertiary care hospital. J Med Microbiol 58: 430-435 [Abstract] [Full Text]  
  • Castanheira, M., Bell, J. M., Turnidge, J. D., Mathai, D., Jones, R. N. (2009). Carbapenem Resistance among Pseudomonas aeruginosa Strains from India: Evidence for Nationwide Endemicity of Multiple Metallo-{beta}-Lactamase Clones (VIM-2, -5, -6, and -11 and the Newly Characterized VIM-18). Antimicrob. Agents Chemother. 53: 1225-1227 [Abstract] [Full Text]  
  • Jacoby, G. A. (2009). AmpC {beta}-Lactamases. Clin. Microbiol. Rev. 22: 161-182 [Abstract] [Full Text]  
  • Juan, C., Beceiro, A., Gutierrez, O., Alberti, S., Garau, M., Perez, J. L., Bou, G., Oliver, A. (2008). Characterization of the New Metallo-{beta}-Lactamase VIM-13 and Its Integron-Borne Gene from a Pseudomonas aeruginosa Clinical Isolate in Spain. Antimicrob. Agents Chemother. 52: 3589-3596 [Abstract] [Full Text]  
  • Li, H., Toleman, M. A., Bennett, P. M., Jones, R. N., Walsh, T. R. (2008). Complete Sequence of p07-406, a 24,179-Base-Pair Plasmid Harboring the blaVIM-7 Metallo-{beta}-Lactamase Gene in a Pseudomonas aeruginosa Isolate from the United States. Antimicrob. Agents Chemother. 52: 3099-3105 [Abstract] [Full Text]  
  • Marchiaro, P., Ballerini, V., Spalding, T., Cera, G., Mussi, M. A., Moran-Barrio, J., Vila, A. J., Viale, A. M., Limansky, A. S. (2008). A convenient microbiological assay employing cell-free extracts for the rapid characterization of Gram-negative carbapenemase producers. J Antimicrob Chemother 62: 336-344 [Abstract] [Full Text]  
  • Schneider, I., Keuleyan, E., Rasshofer, R., Markovska, R., Queenan, A. M., Bauernfeind, A. (2008). VIM-15 and VIM-16, Two New VIM-2-Like Metallo-{beta}-Lactamases in Pseudomonas aeruginosa Isolates from Bulgaria and Germany. Antimicrob. Agents Chemother. 52: 2977-2979 [Abstract] [Full Text]  
  • Mastoraki, A., Douka, E., Kriaras, I., Stravopodis, G., Saroglou, G., Geroulanos, S. (2008). Preventing strategy of multidrug-resistant Acinetobacter baumanii susceptible only to colistin in cardiac surgical intensive care units. Eur. J. Cardiothorac. Surg. 33: 1086-1090 [Abstract] [Full Text]  
  • Marchiaro, P., Tomatis, P. E., Mussi, M. A., Pasteran, F., Viale, A. M., Limansky, A. S., Vila, A. J. (2008). Biochemical Characterization of Metallo-{beta}-Lactamase VIM-11 from a Pseudomonas aeruginosa Clinical Strain. Antimicrob. Agents Chemother. 52: 2250-2252 [Abstract] [Full Text]  
  • Giakkoupi, P., Vourli, S., Polemis, M., Kalapothaki, V., Tzouvelekis, L. S., Vatopoulos, A. C. (2008). Supplementation of Growth Media with Zn2+ Facilitates Detection of VIM-2-Producing Pseudomonas aeruginosa. J. Clin. Microbiol. 46: 1568-1569 [Full Text]  
  • Aschbacher, R., Doumith, M., Livermore, D. M., Larcher, C., Woodford, N. (2008). Linkage of acquired quinolone resistance (qnrS1) and metallo-{beta}-lactamase (blaVIM-1) genes in multiple species of Enterobacteriaceae from Bolzano, Italy. J Antimicrob Chemother 61: 515-523 [Abstract] [Full Text]  
  • Tokatlidou, D., Tsivitanidou, M., Pournaras, S., Ikonomidis, A., Tsakris, A., Sofianou, D. (2008). Outbreak Caused by a Multidrug-Resistant Klebsiella pneumoniae Clone Carrying blaVIM-12 in a University Hospital. J. Clin. Microbiol. 46: 1005-1008 [Abstract] [Full Text]  
  • Biendo, M., Canarelli, B., Thomas, D., Rousseau, F., Hamdad, F., Adjide, C., Laurans, G., Eb, F. (2008). Successive Emergence of Extended-Spectrum {beta}-Lactamase-Producing and Carbapenemase-Producing Enterobacter aerogenes Isolates in a University Hospital. J. Clin. Microbiol. 46: 1037-1044 [Abstract] [Full Text]  
  • Yakupogullari, Y., Poirel, L., Bernabeu, S., Kizirgil, A., Nordmann, P. (2008). Multidrug-resistant Pseudomonas aeruginosa isolate co-expressing extended-spectrum -lactamase PER-1 and metallo- -lactamase VIM-2 from Turkey. J Antimicrob Chemother 61: 221-222 [Full Text]  
  • Gutierrez, O., Juan, C., Cercenado, E., Navarro, F., Bouza, E., Coll, P., Perez, J. L., Oliver, A. (2007). Molecular Epidemiology and Mechanisms of Carbapenem Resistance in Pseudomonas aeruginosa Isolates from Spanish Hospitals. Antimicrob. Agents Chemother. 51: 4329-4335 [Abstract] [Full Text]  
  • Queenan, A. M., Bush, K. (2007). Carbapenemases: the Versatile {beta}-Lactamases. Clin. Microbiol. Rev. 20: 440-458 [Abstract] [Full Text]  
  • Maniati, M., Ikonomidis, A., Mantzana, P., Daponte, A., Maniatis, A. N., Pournaras, S. (2007). A highly carbapenem-resistant Pseudomonas aeruginosa isolate with a novel blaVIM-4/blaP1b integron overexpresses two efflux pumps and lacks OprD. J Antimicrob Chemother 60: 132-135 [Abstract] [Full Text]  
  • Mendes, R. E., Castanheira, M., Toleman, M. A., Sader, H. S., Jones, R. N., Walsh, T. R. (2007). Characterization of an Integron Carrying blaIMP-1 and a New Aminoglycoside Resistance Gene, aac(6')-31, and Its Dissemination among Genetically Unrelated Clinical Isolates in a Brazilian Hospital. Antimicrob. Agents Chemother. 51: 2611-2614 [Abstract] [Full Text]  
  • Pitout, J. D. D., Chow, B. L., Gregson, D. B., Laupland, K. B., Elsayed, S., Church, D. L. (2007). Molecular Epidemiology of Metallo-{beta}-Lactamase-Producing Pseudomonas aeruginosa in the Calgary Health Region: Emergence of VIM-2-Producing Isolates. J. Clin. Microbiol. 45: 294-298 [Abstract] [Full Text]  
  • Libisch, B., Muzslay, M., Gacs, M., Minarovits, J., Knausz, M., Watine, J., Ternak, G., Kenez, E., Kustos, I., Rokusz, L., Szeles, K., Balogh, B., Fuzi, M. (2006). Molecular Epidemiology of VIM-4 Metallo-{beta}-Lactamase-Producing Pseudomonas sp. Isolates in Hungary. Antimicrob. Agents Chemother. 50: 4220-4223 [Abstract] [Full Text]  
  • Rodloff, A. C., Goldstein, E. J. C., Torres, A. (2006). Two decades of imipenem therapy. J Antimicrob Chemother 58: 916-929 [Abstract] [Full Text]  
  • Yu, Y.-S., Qu, T.-T., Zhou, J.-Y., Wang, J., Li, H.-Y., Walsh, T. R. (2006). Integrons Containing the VIM-2 Metallo-{beta}-Lactamase Gene among Imipenem-Resistant Pseudomonas aeruginosa Strains from Different Chinese Hospitals. J. Clin. Microbiol. 44: 4242-4245 [Abstract] [Full Text]  
  • Horianopoulou, M., Legakis, N. J., Kanellopoulou, M., Lambropoulos, S., Tsakris, A., Falagas, M. E. (2006). Frequency and predictors of colonization of the respiratory tract by VIM-2-producing Pseudomonas aeruginosa in patients of a newly established intensive care unit.. J Med Microbiol 55: 1435-1439 [Abstract] [Full Text]  
  • Yan, J.-J., Hsueh, P.-R., Lu, J.-J., Chang, F.-Y., Ko, W.-C., Wu, J.-J. (2006). Characterization of acquired {beta}-lactamases and their genetic support in multidrug-resistant Pseudomonas aeruginosa isolates in Taiwan: the prevalence of unusual integrons. J Antimicrob Chemother 58: 530-536 [Abstract] [Full Text]  
  • Poirel, L., Cabanne, L., Collet, L., Nordmann, P. (2006). Class II Transposon-Borne Structure Harboring Metallo-{beta}-Lactamase Gene blaVIM-2 in Pseudomonas putida.. Antimicrob. Agents Chemother. 50: 2889-2891 [Abstract] [Full Text]  
  • Galani, I., Souli, M., Koratzanis, E., Chryssouli, Z., Giamarellou, H. (2006). Molecular characterization of an Escherichia coli clinical isolate that produces both metallo-{beta}-lactamase VIM-2 and extended-spectrum {beta}-lactamase GES-7: identification of the In8 integron carrying the blaVIM-2 gene. J Antimicrob Chemother 58: 432-433 [Abstract] [Full Text]  
  • Lagatolla, C., Edalucci, E., Dolzani, L., Riccio, M. L., De Luca, F., Medessi, E., Rossolini, G. M., Tonin, E. A. (2006). Molecular Evolution of Metallo-{beta}-Lactamase-Producing Pseudomonas aeruginosa in a Nosocomial Setting of High-Level Endemicity.. J. Clin. Microbiol. 44: 2348-2353 [Abstract] [Full Text]  
  • Jacoby, G. A., Walsh, K. E., Walker, V. J. (2006). Identification of Extended-Spectrum, AmpC, and Carbapenem- Hydrolyzing {beta}-Lactamases in Escherichia coli and Klebsiella pneumoniae by Disk Tests.. J. Clin. Microbiol. 44: 1971-1976 [Abstract] [Full Text]  
  • Yong, D., Choi, Y. S., Roh, K. H., Kim, C. K., Park, Y. H., Yum, J. H., Lee, K., Chong, Y. (2006). Increasing Prevalence and Diversity of Metallo-{beta}-Lactamases in Pseudomonas spp., Acinetobacter spp., and Enterobacteriaceae from Korea.. Antimicrob. Agents Chemother. 50: 1884-1886 [Abstract] [Full Text]  
  • Fiett, J., Baraniak, A., Mrowka, A., Fleischer, M., Drulis-Kawa, Z., Naumiuk, L., Samet, A., Hryniewicz, W., Gniadkowski, M. (2006). Molecular Epidemiology of Acquired-Metallo-{beta}-Lactamase-Producing Bacteria in Poland. Antimicrob. Agents Chemother. 50: 880-886 [Abstract] [Full Text]  
  • Wang, C.-X., Mi, Z.-H. (2006). Imipenem-resistant Pseudomonas aeruginosa producing IMP-1 metallo-{beta}-lactamases and lacking the outer-membrane protein OprD.. J Med Microbiol 55: 353-354 [Full Text]  
  • Xiong, J., Hynes, M. F., Ye, H., Chen, H., Yang, Y., M'Zali, F., Hawkey, P. M., the Guangzhou Antibiotic Resistance Study Group, (2006). blaIMP-9 and Its Association with Large Plasmids Carried by Pseudomonas aeruginosa Isolates from the People's Republic of China. Antimicrob. Agents Chemother. 50: 355-358 [Abstract] [Full Text]  
  • Marra, A. R., Pereira, C. A. P., Gales, A. C., Menezes, L. C., Cal, R. G. R., de Souza, J. M. A., Edmond, M. B., Faro, C., Wey, S. B. (2006). Bloodstream Infections with Metallo-{beta}-Lactamase-Producing Pseudomonas aeruginosa: Epidemiology, Microbiology, and Clinical Outcomes. Antimicrob. Agents Chemother. 50: 388-390 [Abstract] [Full Text]  
  • Siroy, A., Molle, V., Lemaitre-Guillier, C., Vallenet, D., Pestel-Caron, M., Cozzone, A. J., Jouenne, T., De, E. (2005). Channel Formation by CarO, the Carbapenem Resistance-Associated Outer Membrane Protein of Acinetobacter baumannii. Antimicrob. Agents Chemother. 49: 4876-4883 [Abstract] [Full Text]  
  • Pournaras, S., Ikonomidis, A., Tzouvelekis, L. S., Tokatlidou, D., Spanakis, N., Maniatis, A. N., Legakis, N. J., Tsakris, A. (2005). VIM-12, a Novel Plasmid-Mediated Metallo-{beta}-Lactamase from Klebsiella pneumoniae That Resembles a VIM-1/VIM-2 Hybrid. Antimicrob. Agents Chemother. 49: 5153-5156 [Abstract] [Full Text]  
  • Lee, K., Yum, J. H., Yong, D., Lee, H. M., Kim, H. D., Docquier, J.-D., Rossolini, G. M., Chong, Y. (2005). Novel Acquired Metallo-{beta}-Lactamase Gene, blaSIM-1, in a Class 1 Integron from Acinetobacter baumannii Clinical Isolates from Korea. Antimicrob. Agents Chemother. 49: 4485-4491 [Abstract] [Full Text]  
  • Marchiaro, P., Mussi, M. A., Ballerini, V., Pasteran, F., Viale, A. M., Vila, A. J., Limansky, A. S. (2005). Sensitive EDTA-Based Microbiological Assays for Detection of Metallo-{beta}-Lactamases in Nonfermentative Gram-Negative Bacteria. J. Clin. Microbiol. 43: 5648-5652 [Abstract] [Full Text]  
  • Gacar, G. G., Midilli, K., Kolayli, F., Ergen, K., Gundes, S., Hosoglu, S., Karadenizli, A., Vahaboglu, H. (2005). Genetic and Enzymatic Properties of Metallo-{beta}-Lactamase VIM-5 from a Clinical Isolate of Enterobacter cloacae. Antimicrob. Agents Chemother. 49: 4400-4403 [Abstract] [Full Text]  
  • Ikonomidis, A., Tokatlidou, D., Kristo, I., Sofianou, D., Tsakris, A., Mantzana, P., Pournaras, S., Maniatis, A. N. (2005). Outbreaks in Distinct Regions Due to a Single Klebsiella pneumoniae Clone Carrying a blaVIM-1 Metallo-{beta}-Lactamase Gene. J. Clin. Microbiol. 43: 5344-5347 [Abstract] [Full Text]  
  • Horii, T., Muramatsu, H., Iinuma, Y. (2005). Mechanisms of resistance to fluoroquinolones and carbapenems in Pseudomonas putida. J Antimicrob Chemother 56: 643-647 [Abstract] [Full Text]  
  • Vedel, G. (2005). Simple method to determine {beta}-lactam resistance phenotypes in Pseudomonas aeruginosa using the disc agar diffusion test. J Antimicrob Chemother 56: 657-664 [Abstract] [Full Text]  
  • Poirel, L., Brinas, L., Verlinde, A., Ide, L., Nordmann, P. (2005). BEL-1, a Novel Clavulanic Acid-Inhibited Extended-Spectrum {beta}-Lactamase, and the Class 1 Integron In120 in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 49: 3743-3748 [Abstract] [Full Text]  
  • Furushita, M., Okamoto, A., Maeda, T., Ohta, M., Shiba, T. (2005). Isolation of Multidrug-Resistant Stenotrophomonas maltophilia from Cultured Yellowtail (Seriola quinqueradiata) from a Marine Fish Farm. Appl. Environ. Microbiol. 71: 5598-5600 [Abstract] [Full Text]  
  • Guerin, F., Henegar, C., Spiridon, G., Launay, O., Salmon-Ceron, D., Poyart, C. (2005). Bacterial prostatitis due to Pseudomonas aeruginosa harbouring the blaVIM-2 metallo-{beta}-lactamase gene from Saudi Arabia. J Antimicrob Chemother 56: 601-602 [Full Text]  
  • Tortola, M. T., Lavilla, S., Miro, E., Gonzalez, J. J., Larrosa, N., Sabate, M., Navarro, F., Prats, G. (2005). First Detection of a Carbapenem-Hydrolyzing Metalloenzyme in Two Enterobacteriaceae Isolates in Spain. Antimicrob. Agents Chemother. 49: 3492-3494 [Abstract] [Full Text]  
  • Lolans, K., Queenan, A. M., Bush, K., Sahud, A., Quinn, J. P. (2005). First Nosocomial Outbreak of Pseudomonas aeruginosa Producing an Integron-Borne Metallo-{beta}-Lactamase (VIM-2) in the United States. Antimicrob. Agents Chemother. 49: 3538-3540 [Abstract] [Full Text]  
  • Dubois, V., Arpin, C., Noury, P., Andre, C., Coulange, L., Quentin, C. (2005). Prolonged Outbreak of Infection Due to TEM-21-Producing Strains of Pseudomonas aeruginosa and Enterobacteria in a Nursing Home. J. Clin. Microbiol. 43: 4129-4138 [Abstract] [Full Text]  
  • Poirel, L., Van De Loo, M., Mammeri, H., Nordmann, P. (2005). Association of Plasmid-Mediated Quinolone Resistance with Extended-Spectrum {beta}-Lactamase VEB-1. Antimicrob. Agents Chemother. 49: 3091-3094 [Abstract] [Full Text]  
  • Pitout, J. D. D., Gregson, D. B., Poirel, L., McClure, J.-A., Le, P., Church, D. L. (2005). Detection of Pseudomonas aeruginosa Producing Metallo-{beta}-Lactamases in a Large Centralized Laboratory. J. Clin. Microbiol. 43: 3129-3135 [Abstract] [Full Text]  
  • Yamaguchi, Y., Kuroki, T., Yasuzawa, H., Higashi, T., Jin, W., Kawanami, A., Yamagata, Y., Arakawa, Y., Goto, M., Kurosaki, H. (2005). Probing the Role of Asp-120(81) of Metallo-{beta}-lactamase (IMP-1) by Site-directed Mutagenesis, Kinetic Studies, and X-ray Crystallography. J. Biol. Chem. 280: 20824-20832 [Abstract] [Full Text]  
  • Quinteira, S., Ferreira, H., Peixe, L. (2005). First Isolation of blaVIM-2 in an Environmental Isolate of Pseudomonas pseudoalcaligenes. Antimicrob. Agents Chemother. 49: 2140-2141 [Full Text]  
  • Galani, I., Souli, M., Chryssouli, Z., Orlandou, K., Giamarellou, H. (2005). Characterization of a new integron containing blaVIM-1 and aac(6')-IIc in an Enterobacter cloacae clinical isolate from Greece. J Antimicrob Chemother 55: 634-638 [Abstract] [Full Text]  
  • Walsh, T. R., Toleman, M. A., Poirel, L., Nordmann, P. (2005). Metallo-{beta}-Lactamases: the Quiet before the Storm?. Clin. Microbiol. Rev. 18: 306-325 [Abstract] [Full Text]  
  • Deplano, A., Denis, O., Poirel, L., Hocquet, D., Nonhoff, C., Byl, B., Nordmann, P., Vincent, J. L., Struelens, M. J. (2005). Molecular Characterization of an Epidemic Clone of Panantibiotic-Resistant Pseudomonas aeruginosa. J. Clin. Microbiol. 43: 1198-1204 [Abstract] [Full Text]  
  • Riccio, M. L., Pallecchi, L., Docquier, J.-D., Cresti, S., Catania, M. R., Pagani, L., Lagatolla, C., Cornaglia, G., Fontana, R., Rossolini, G. M. (2005). Clonal Relatedness and Conserved Integron Structures in Epidemiologically Unrelated Pseudomonas aeruginosa Strains Producing the VIM-1 Metallo-{beta}-Lactamase from Different Italian Hospitals. Antimicrob. Agents Chemother. 49: 104-110 [Abstract] [Full Text]  
  • Quinteira, S., Sousa, J. C., Peixe, L. (2005). Characterization of In100, a New Integron Carrying a Metallo-{beta}-Lactamase and a Carbenicillinase, from Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 49: 451-453 [Abstract] [Full Text]  
  • Conceicao, T., Brizio, A., Duarte, A., Barros, R. (2005). First Isolation of blaVIM-2 in Klebsiella oxytoca Clinical Isolates from Portugal. Antimicrob. Agents Chemother. 49: 476-476 [Full Text]  
  • Toleman, M. A., Biedenbach, D., Bennett, D. M. C., Jones, R. N., Walsh, T. R. (2005). Italian metallo-{beta}-lactamases: a national problem? Report from the SENTRY Antimicrobial Surveillance Programme. J Antimicrob Chemother 55: 61-70 [Abstract] [Full Text]  
  • Castanheira, M., Toleman, M. A., Jones, R. N., Schmidt, F. J., Walsh, T. R. (2004). Molecular Characterization of a {beta}-Lactamase Gene, blaGIM-1, Encoding a New Subclass of Metallo-{beta}-Lactamase. Antimicrob. Agents Chemother. 48: 4654-4661 [Abstract] [Full Text]  
  • Mendes, R. E., Toleman, M. A., Ribeiro, J., Sader, H. S., Jones, R. N., Walsh, T. R. (2004). Integron Carrying a Novel Metallo-{beta}-Lactamase Gene, blaIMP-16, and a Fused Form of Aminoglycoside-Resistant Gene aac(6')-30/aac(6')-Ib': Report from the SENTRY Antimicrobial Surveillance Program. Antimicrob. Agents Chemother. 48: 4693-4702 [Abstract] [Full Text]  
  • Lartigue, M.-F., Poirel, L., Nordmann, P. (2004). First Detection of a Carbapenem-Hydrolyzing Metalloenzyme in an Enterobacteriaceae Isolate in France. Antimicrob. Agents Chemother. 48: 4929-4930 [Full Text]  
  • Nishio, H., Komatsu, M., Shibata, N., Shimakawa, K., Sueyoshi, N., Ura, T., Satoh, K., Toyokawa, M., Nakamura, T., Wada, Y., Orita, T., Kofuku, T., Yamasaki, K., Sakamoto, M., Kinoshita, S., Aihara, M., Arakawa, Y. (2004). Metallo-{beta}-Lactamase-Producing Gram-Negative Bacilli: Laboratory-Based Surveillance in Cooperation with 13 Clinical Laboratories in the Kinki Region of Japan. J. Clin. Microbiol. 42: 5256-5263 [Abstract] [Full Text]  
  • Garau, G., Garcia-Saez, I., Bebrone, C., Anne, C., Mercuri, P., Galleni, M., Frere, J.-M., Dideberg, O. (2004). Update of the Standard Numbering Scheme for Class B {beta}-Lactamases. Antimicrob. Agents Chemother. 48: 2347-2349 [Full Text]  
  • Wachino, J.-i., Doi, Y., Yamane, K., Shibata, N., Yagi, T., Kubota, T., Ito, H., Arakawa, Y. (2004). Nosocomial Spread of Ceftazidime-Resistant Klebsiella pneumoniae Strains Producing a Novel Class A {beta}-Lactamase, GES-3, in a Neonatal Intensive Care Unit in Japan. Antimicrob. Agents Chemother. 48: 1960-1967 [Abstract] [Full Text]  
  • Girlich, D., Naas, T., Nordmann, P. (2004). Biochemical Characterization of the Naturally Occurring Oxacillinase OXA-50 of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 48: 2043-2048 [Abstract] [Full Text]  
  • Koh, T. H., Wang, G. C. Y., Sng, L.-H. (2004). IMP-1 and a Novel Metallo-{beta}-Lactamase, VIM-6, in Fluorescent Pseudomonads Isolated in Singapore. Antimicrob. Agents Chemother. 48: 2334-2336 [Abstract] [Full Text]  
  • Poirel, L., Magalhaes, M., Lopes, M., Nordmann, P. (2004). Molecular Analysis of Metallo-{beta}-Lactamase Gene blaSPM-1-Surrounding Sequences from Disseminated Pseudomonas aeruginosa Isolates in Recife, Brazil. Antimicrob. Agents Chemother. 48: 1406-1409 [Abstract] [Full Text]  
  • Mendes, R. E., Castanheira, M., Garcia, P., Guzman, M., Toleman, M. A., Walsh, T. R., Jones, R. N. (2004). First Isolation of blaVIM-2 in Latin America: Report from the SENTRY Antimicrobial Surveillance Program. Antimicrob. Agents Chemother. 48: 1433-1434 [Full Text]  
  • Patzer, J., Toleman, M. A., Deshpande, L. M., Kaminska, W., Dzierzanowska, D., Bennett, P. M., Jones, R. N., Walsh, T. R. (2004). Pseudomonas aeruginosa strains harbouring an unusual blaVIM-4 gene cassette isolated from hospitalized children in Poland (1998-2001). J Antimicrob Chemother 53: 451-456 [Abstract] [Full Text]  
  • Yatsuyanagi, J., Saito, S., Harata, S., Suzuki, N., Ito, Y., Amano, K.-i., Enomoto, K. (2004). Class 1 Integron Containing Metallo-{beta}-Lactamase Gene blaVIM-2 in Pseudomonas aeruginosa Clinical Strains Isolated in Japan. Antimicrob. Agents Chemother. 48: 626-628 [Abstract] [Full Text]  
  • Luzzaro, F., Docquier, J.-D., Colinon, C., Endimiani, A., Lombardi, G., Amicosante, G., Rossolini, G. M., Toniolo, A. (2004). Emergence in Klebsiella pneumoniae and Enterobacter cloacae Clinical Isolates of the VIM-4 Metallo-{beta}-Lactamase Encoded by a Conjugative Plasmid. Antimicrob. Agents Chemother. 48: 648-650 [Abstract] [Full Text]  
  • Toleman, M. A., Rolston, K., Jones, R. N., Walsh, T. R. (2004). blaVIM-7, an Evolutionarily Distinct Metallo-{beta}-Lactamase Gene in a Pseudomonas aeruginosa Isolate from the United States. Antimicrob. Agents Chemother. 48: 329-332 [Abstract] [Full Text]  
  • Shibata, N., Doi, Y., Yamane, K., Yagi, T., Kurokawa, H., Shibayama, K., Kato, H., Kai, K., Arakawa, Y. (2003). PCR Typing of Genetic Determinants for Metallo-{beta}-Lactamases and Integrases Carried by Gram-Negative Bacteria Isolated in Japan, with Focus on the Class 3 Integron. J. Clin. Microbiol. 41: 5407-5413 [Abstract] [Full Text]  
  • Lee, K., Lim, Y. S., Yong, D., Yum, J. H., Chong, Y. (2003). Evaluation of the Hodge Test and the Imipenem-EDTA Double-Disk Synergy Test for Differentiating Metallo-{beta}-Lactamase-Producing Isolates of Pseudomonas spp. and Acinetobacter spp.. J. Clin. Microbiol. 41: 4623-4629 [Abstract] [Full Text]  
  • Yomoda, S., Okubo, T., Takahashi, A., Murakami, M., Iyobe, S. (2003). Presence of Pseudomonas putida Strains Harboring Plasmids Bearing the Metallo-{beta}-Lactamase Gene blaIMP in a Hospital in Japan. J. Clin. Microbiol. 41: 4246-4251 [Abstract] [Full Text]  
  • Walsh, T. R., Toleman, M. A., Hryniewicz, W., Bennett, P. M., Jones, R. N. (2003). Evolution of an integron carrying blaVIM-2 in Eastern Europe: report from the SENTRY Antimicrobial Surveillance Program. J Antimicrob Chemother 52: 116-119 [Abstract] [Full Text]  
  • Materon, I. C., Queenan, A. M., Koehler, T. M., Bush, K., Palzkill, T. (2003). Biochemical Characterization of {beta}-Lactamases Bla1 and Bla2 from Bacillus anthracis. Antimicrob. Agents Chemother. 47: 2040-2042 [Abstract] [Full Text]  
  • Pournaras, S., Maniati, M., Petinaki, E., Tzouvelekis, L. S., Tsakris, A., Legakis, N. J., Maniatis, A. N. (2003). Hospital outbreak of multiple clones of Pseudomonas aeruginosa carrying the unrelated metallo-{beta}-lactamase gene variants blaVIM-2 and blaVIM-4. J Antimicrob Chemother 51: 1409-1414 [Abstract] [Full Text]  
  • Riccio, M. L., Docquier, J.-D., Dell'Amico, E., Luzzaro, F., Amicosante, G., Rossolini, G. M. (2003). Novel 3-N-Aminoglycoside Acetyltransferase Gene, aac(3)-Ic, from a Pseudomonas aeruginosa Integron. Antimicrob. Agents Chemother. 47: 1746-1748 [Abstract] [Full Text]  
  • Murphy, T. A., Simm, A. M., Toleman, M. A., Jones, R. N., Walsh, T. R. (2003). Biochemical Characterization of the Acquired Metallo-{beta}-Lactamase SPM-1 from Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 47: 582-587 [Abstract] [Full Text]  
  • Poirel, L., Heritier, C., Podglajen, I., Sougakoff, W., Gutmann, L., Nordmann, P. (2003). Emergence in Klebsiella pneumoniae of a Chromosome-Encoded SHV {beta}-Lactamase That Compromises the Efficacy of Imipenem. Antimicrob. Agents Chemother. 47: 755-758 [Abstract] [Full Text]  
  • Docquier, J.-D., Lamotte-Brasseur, J., Galleni, M., Amicosante, G., Frere, J.-M., Rossolini, G. M. (2003). On functional and structural heterogeneity of VIM-type metallo-{beta}-lactamases. J Antimicrob Chemother 51: 257-266 [Abstract] [Full Text]  
  • Jeong, S. H., Lee, K., Chong, Y., Yum, J. H., Lee, S. H., Choi, H. J., Kim, J. M., Park, K. H., Han, B. H., Lee, S. W., Jeong, T. S. (2003). Characterization of a new integron containing VIM-2, a metallo- {beta}-lactamase gene cassette, in a clinical isolate of Enterobacter cloacae. J Antimicrob Chemother 51: 397-400 [Abstract] [Full Text]  
  • Miriagou, V., Tzelepi, E., Gianneli, D., Tzouvelekis, L. S. (2003). Escherichia coli with a Self-Transferable, Multiresistant Plasmid Coding for Metallo-{beta}-Lactamase VIM-1. Antimicrob. Agents Chemother. 47: 395-397 [Abstract] [Full Text]  
  • Pournaras, S., Tsakris, A., Maniati, M., Tzouvelekis, L. S., Maniatis, A. N. (2002). Novel Variant (blaVIM-4) of the Metallo-{beta}-Lactamase Gene blaVIM-1 in a Clinical Strain of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 46: 4026-4028 [Abstract] [Full Text]  
  • Lopez-Otsoa, F., Gallego, L., Towner, K. J., Tysall, L., Woodford, N., Livermore, D. M. (2002). Endemic Carbapenem Resistance Associated with OXA-40 Carbapenemase among Acinetobacter baumannii Isolates from a Hospital in Northern Spain. J. Clin. Microbiol. 40: 4741-4743 [Abstract] [Full Text]  
  • Dubois, V., Arpin, C., Noury, P., Quentin, C. (2002). Clinical Strain of Pseudomonas aeruginosa Carrying a blaTEM-21 Gene Located on a Chromosomal Interrupted TnA Type Transposon. Antimicrob. Agents Chemother. 46: 3624-3626 [Abstract] [Full Text]