Previous Article | Next Article 
Antimicrobial Agents and Chemotherapy, May 2000, p. 1315-1321, Vol. 44, No. 5
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Distribution and Content of Class 1 Integrons in
Different Vibrio cholerae O-Serotype Strains Isolated
in Thailand
Anders
Dalsgaard,1,*
Anita
Forslund,1
Oralak
Serichantalergs,2 and
Dorthe
Sandvang1,3
Department of Veterinary Microbiology, Royal
Veterinary and Agricultural University, Frederiksberg, DK-1870
Frederiksberg C,1 and Danish Veterinary
Laboratory, DK-1790 Copenhagen V,3 and
Armed Forces Research Institute of Medical Sciences, Bangkok
10400, Thailand2
Received 1 June 1999/Returned for modification 26 July
1999/Accepted 8 February 2000
In this study, 176 clinical and environmental Vibrio
cholerae strains of different O serotypes isolated in Thailand
from 1982 to 1995 were selected and studied for the presence of class 1 integrons, a new group of genetic elements which carry antibiotic resistance genes. Using PCR and DNA sequencing, we found that 44 isolates contained class 1 integrons harboring the aadB,
aadA2, blaP1, dfrA1, and
dfrA15 gene cassettes, which encode resistance to
gentamicin, kanamycin, and tobramycin; streptomycin and spectinomycin;
-lactams; and trimethoprim, respectively. Each cassette array contained only a single antibiotic resistance gene. Although resistance genes in class 1 integrons were found in strains from the same epidemic, as well as in unrelated non-O1, non-O139 strains isolated from children with diarrhea, they were found to encode only some of the
antibiotic resistance expressed by the strains. Serotype O139 strains
did not contain class 1 integrons. However, the appearance and
disappearance of the O139 serotype in the coastal city Samutsakorn in
1992 and 1993 were associated with the emergence of a distinct V. cholerae O1 strain which contained the aadA2
resistance gene cassette. A 150-kb self-transmissible plasmid found in
three O1 strains isolated in 1982 contained the aadB gene
cassette. Surprisingly, several strains harbored two integrons
containing different cassettes. Thus, class 1 integrons containing
various resistance gene cassettes are distributed among different
V. cholerae O serotypes of mainly clinical origin in Thailand.
*
Corresponding author. Mailing address: Department of
Veterinary Microbiology, The Royal Veterinary and Agricultural
University, Bülowsvej 13, 1870 Frederiksberg C, Denmark. Phone:
45-35-282720. Fax: 45-35-282757. E-mail: ad{at}kvl.dk.
Antimicrobial Agents and Chemotherapy, May 2000, p. 1315-1321, Vol. 44, No. 5
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Khan, A. A., Ponce, E., Nawaz, M. S., Cheng, C.-M., Khan, J. A., West, C. S.
(2009). Identification and Characterization of Class 1 Integron Resistance Gene Cassettes among Salmonella Strains Isolated from Imported Seafood. Appl. Environ. Microbiol.
75: 1192-1196
[Abstract]
[Full Text]
-
Pourshafie, M. R., Bakhshi, B., Ranjbar, R., Sedaghat, M., Sadeghifard, N., Zaemi Yazdi, J., Parzadeh, M., Raesi, J.
(2007). Dissemination of a single Vibrio cholerae clone in cholera outbreaks during 2005 in Iran. J Med Microbiol
56: 1615-1619
[Abstract]
[Full Text]
-
Pan, J.-C., Ye, R., Meng, D.-M., Zhang, W., Wang, H.-Q., Liu, K.-Z.
(2006). Molecular characteristics of class 1 and class 2 integrons and their relationships to antibiotic resistance in clinical isolates of Shigella sonnei and Shigella flexneri. J Antimicrob Chemother
58: 288-296
[Abstract]
[Full Text]
-
Srinivasan, V. B., Virk, R. K., Kaundal, A., Chakraborty, R., Datta, B., Ramamurthy, T., Mukhopadhyay, A. K., Ghosh, A.
(2006). Mechanism of Drug Resistance in Clonally Related Clinical Isolates of Vibrio fluvialis Isolated in Kolkata, India.. Antimicrob. Agents Chemother.
50: 2428-2432
[Abstract]
[Full Text]
-
Ceccarelli, D., Salvia, A. M., Sami, J., Cappuccinelli, P., Colombo, M. M.
(2006). New Cluster of Plasmid-Located Class 1 Integrons in Vibrio cholerae O1 and a dfrA15 Cassette-Containing Integron in Vibrio parahaemolyticus Isolated in Angola.. Antimicrob. Agents Chemother.
50: 2493-2499
[Abstract]
[Full Text]
-
Shi, L., Fujihara, K., Sato, T., Ito, H., Garg, P., Chakrabarty, R., Ramamurthy, T., Nair, G. B., Takeda, Y., Yamasaki, S.
(2006). Distribution and characterization of integrons in various serogroups of Vibrio cholerae strains isolated from diarrhoeal patients between 1992 and 2000 in Kolkata, India.. J Med Microbiol
55: 575-583
[Abstract]
[Full Text]
-
Vora, G. J., Meador, C. E., Bird, M. M., Bopp, C. A., Andreadis, J. D., Stenger, D. A.
(2005). Microarray-based detection of genetic heterogeneity, antimicrobial resistance, and the viable but nonculturable state in human pathogenic Vibrio spp.. Proc. Natl. Acad. Sci. USA
102: 19109-19114
[Abstract]
[Full Text]
-
Kehrenberg, C., Catry, B., Haesebrouck, F., de Kruif, A., Schwarz, S.
(2005). Novel Spectinomycin/Streptomycin Resistance Gene, aadA14, from Pasteurella multocida. Antimicrob. Agents Chemother.
49: 3046-3049
[Abstract]
[Full Text]
-
Toleman, M. A., Biedenbach, D., Bennett, D. M. C., Jones, R. N., Walsh, T. R.
(2005). Italian metallo-{beta}-lactamases: a national problem? Report from the SENTRY Antimicrobial Surveillance Programme. J Antimicrob Chemother
55: 61-70
[Abstract]
[Full Text]
-
Petroni, A., Melano, R. G., Saka, H. A., Garutti, A., Mange, L., Pasteran, F., Rapoport, M., Miranda, M., Faccone, D., Rossi, A., Hoffman, P. S., Galas, M. F.
(2004). CARB-9, a Carbenicillinase Encoded in the VCR Region of Vibrio cholerae Non-O1, Non-O139 Belongs to a Family of Cassette-Encoded {beta}-Lactamases. Antimicrob. Agents Chemother.
48: 4042-4046
[Abstract]
[Full Text]
-
Vakulenko, S. B., Mobashery, S.
(2003). Versatility of Aminoglycosides and Prospects for Their Future. Clin. Microbiol. Rev.
16: 430-450
[Abstract]
[Full Text]
-
Thungapathra, M., Amita, , Sinha, K. K., Chaudhuri, S. R., Garg, P., Ramamurthy, T., Nair, G. B., Ghosh, A.
(2002). Occurrence of Antibiotic Resistance Gene Cassettes aac(6')-Ib, dfrA5, dfrA12, and ereA2 in Class I Integrons in Non-O1, Non-O139 Vibrio cholerae Strains in India. Antimicrob. Agents Chemother.
46: 2948-2955
[Abstract]
[Full Text]
-
Melano, R., Petroni, A., Garutti, A., Saka, H. A., Mange, L., Pasteran, F., Rapoport, M., Rossi, A., Galas, M.
(2002). New Carbenicillin-Hydrolyzing {beta}-Lactamase (CARB-7) from Vibrio cholerae Non-O1, Non-O139 Strains Encoded by the VCR Region of the V. cholerae Genome. Antimicrob. Agents Chemother.
46: 2162-2168
[Abstract]
[Full Text]
-
Partridge, S. R., Brown, H. J., Hall, R. M.
(2002). Characterization and Movement of the Class 1 Integron Known as Tn2521 and Tn1405. Antimicrob. Agents Chemother.
46: 1288-1294
[Abstract]
[Full Text]
-
Dalsgaard, A., Forslund, A., Sandvang, D., Arntzen, L., Keddy, K.
(2001). Vibrio cholerae O1 outbreak isolates in Mozambique and South Africa in 1998 are multiple-drug resistant, contain the SXT element and the aadA2 gene located on class 1 integrons. J Antimicrob Chemother
48: 827-838
[Abstract]
[Full Text]
-
Hochhut, B., Lotfi, Y., Mazel, D., Faruque, S. M., Woodgate, R., Waldor, M. K.
(2001). Molecular Analysis of Antibiotic Resistance Gene Clusters in Vibrio cholerae O139 and O1 SXT Constins. Antimicrob. Agents Chemother.
45: 2991-3000
[Abstract]
[Full Text]
-
Girlich, D., Poirel, L., Leelaporn, A., Karim, A., Tribuddharat, C., Fennewald, M., Nordmann, P.
(2001). Molecular Epidemiology of the Integron-Located VEB-1 Extended-Spectrum {beta}-Lactamase in Nosocomial Enterobacterial Isolates in Bangkok, Thailand. J. Clin. Microbiol.
39: 175-182
[Abstract]
[Full Text]
-
Dalsgaard, A., Forslund, A., Petersen, A., Brown, D. J., Dias, F., Monteiro, S., Molbak, K., Aaby, P., Rodrigues, A., Sandström, A.
(2000). Class 1 Integron-Borne, Multiple-Antibiotic Resistance Encoded by a 150-Kilobase Conjugative Plasmid in Epidemic Vibrio cholerae O1 Strains Isolated in Guinea-Bissau. J. Clin. Microbiol.
38: 3774-3779
[Abstract]
[Full Text]