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Antimicrobial Agents and Chemotherapy, May 2000, p. 1315-1321, Vol. 44, No. 5
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Distribution and Content of Class 1 Integrons in Different Vibrio cholerae O-Serotype Strains Isolated in Thailand

Anders Dalsgaard,1,* Anita Forslund,1 Oralak Serichantalergs,2 and Dorthe Sandvang1,3

Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Frederiksberg, DK-1870 Frederiksberg C,1 and Danish Veterinary Laboratory, DK-1790 Copenhagen V,3 and Armed Forces Research Institute of Medical Sciences, Bangkok 10400, Thailand2

Received 1 June 1999/Returned for modification 26 July 1999/Accepted 8 February 2000

In this study, 176 clinical and environmental Vibrio cholerae strains of different O serotypes isolated in Thailand from 1982 to 1995 were selected and studied for the presence of class 1 integrons, a new group of genetic elements which carry antibiotic resistance genes. Using PCR and DNA sequencing, we found that 44 isolates contained class 1 integrons harboring the aadB, aadA2, blaP1, dfrA1, and dfrA15 gene cassettes, which encode resistance to gentamicin, kanamycin, and tobramycin; streptomycin and spectinomycin; beta -lactams; and trimethoprim, respectively. Each cassette array contained only a single antibiotic resistance gene. Although resistance genes in class 1 integrons were found in strains from the same epidemic, as well as in unrelated non-O1, non-O139 strains isolated from children with diarrhea, they were found to encode only some of the antibiotic resistance expressed by the strains. Serotype O139 strains did not contain class 1 integrons. However, the appearance and disappearance of the O139 serotype in the coastal city Samutsakorn in 1992 and 1993 were associated with the emergence of a distinct V. cholerae O1 strain which contained the aadA2 resistance gene cassette. A 150-kb self-transmissible plasmid found in three O1 strains isolated in 1982 contained the aadB gene cassette. Surprisingly, several strains harbored two integrons containing different cassettes. Thus, class 1 integrons containing various resistance gene cassettes are distributed among different V. cholerae O serotypes of mainly clinical origin in Thailand.

* Corresponding author. Mailing address: Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Bülowsvej 13, 1870 Frederiksberg C, Denmark. Phone: 45-35-282720. Fax: 45-35-282757. E-mail: ad{at}kvl.dk.

Antimicrobial Agents and Chemotherapy, May 2000, p. 1315-1321, Vol. 44, No. 5
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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