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Antimicrobial Agents and Chemotherapy, May 2000, p. 1346-1348, Vol. 44, No. 5
Unité des Agents Antibactériens,
Institut Pasteur, 75724 Paris Cedex 15, France,1
and Department of Biochemistry, University of Cambridge,
Cambridge, United Kingdom, CB2 1QW2
Received 30 July 1999/Returned for modification 26 November
1999/Accepted 19 January 2000
VanD-type Enterococcus faecium BM4416 was
constitutively resistant to vancomycin and to teicoplanin by synthesis
of peptidoglycan precursors ending in
D-alanyl-D-lactate. Like E. faecium BM4339, the only VanD-type strain described so far,
BM4416 produced an impaired D-alanine:D-alanine
ligase. Unlike for BM4339, which had a 5-bp insertion in
ddl, inactivation of the gene in BM4416 was due to
insertion of IS19.
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Glycopeptide-Resistant Enterococcus
faecium BM4416 Is a VanD-Type Strain with an Impaired
D-Alanine:D-Alanine Ligase
*
Corresponding author. Mailing address: Unité des
Agents Antibactériens, Institut Pasteur, 25, rue du Docteur Roux,
75724 Paris Cedex 15, France. Phone: (33) (1) 45 68 83 18. Fax: (33) (1) 45 68 83 19. E-mail: brunoper{at}pasteur.fr.
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