Prevalence of SHV-12 among Clinical Isolates of Klebsiella pneumoniae Producing Extended-Spectrum beta -Lactamases and Identification of a Novel AmpC Enzyme (CMY-8) in Southern Taiwan

doi:10.1128/AAC.44.6.1438-1442.2000

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Antimicrobial Agents and Chemotherapy, June 2000, p. 1438-1442, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Prevalence of SHV-12 among Clinical Isolates of Klebsiella pneumoniae Producing Extended-Spectrum $beta$ -Lactamases and Identification of a Novel AmpC Enzyme (CMY-8) in Southern Taiwan

Jing-Jou Yan,¹ Shiou-Mei Wu,² Shu-Huei Tsai,¹ Jiunn-Jong Wu,²^,^* and Ih-Jen Su¹

Department of Pathology,¹ National Cheng Kung University Medical Center, and Department of Medical Technology,² National Cheng Kung University Medical College, Tainan, Taiwan

Received 19 October 1999/Returned for modification 26 January 2000/Accepted 29 February 2000

Twenty (8.5%) of 234 nonrepetitive clinical isolates of Klebsiella pneumoniae from southern Taiwan were found to produce extended-spectrum $beta$ -lactamases (ESBLs): 10 strains produced SHV-12, 4 produced SHV-5,2 produced a non-TEM non-SHV ESBL with a pI of 8.3, 3 produceda novel AmpC $beta$ -lactamase designated CMY-8 with a pI of 8.25, and1 produced SHV-12 and an unidentified AmpC enzyme with a pI of8.2. The CMY-8 enzyme confers a resistance phenotype similar toCMY-1 and MOX-1, and sequence comparisons showed high homologies(>95%) of nucleotide and amino acid sequences among these threeenzymes. Plasmid and pulse-field gel electrophoresis analysesrevealed that all isolates harboring an SHV-derived ESBL weregenetically unrelated, indicating that dissemination of resistanceplasmids is responsible for the spread of SHV ESBLs among K. pneumoniaein this area. All three isolates carrying CMY-8 had identicalgenotypic patterns, suggesting the presence of an epidemicstrain.

^* Corresponding author. Mailing address: Department of Medical Technology, National Cheng Kung University Medical College, No.1 University Rd., Tainan, Taiwan 70101, Taiwan. Phone: 886-6-2353535,ext. 5775. Fax: 886-6-2363956. E-mail: jjwu{at}mail.ncku.edu.tw.

Antimicrobial Agents and Chemotherapy, June 2000, p. 1438-1442, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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Prevalence of SHV-12 among Clinical Isolates of Klebsiella pneumoniae Producing Extended-Spectrum -Lactamases and Identification of a Novel AmpC Enzyme (CMY-8) in Southern Taiwan

Prevalence of SHV-12 among Clinical Isolates of Klebsiella pneumoniae Producing Extended-Spectrum $beta$ -Lactamases and Identification of a Novel AmpC Enzyme (CMY-8) in Southern Taiwan