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Antimicrobial Agents and Chemotherapy, June 2000, p. 1438-1442, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Prevalence of SHV-12 among Clinical Isolates of Klebsiella pneumoniae Producing Extended-Spectrum beta -Lactamases and Identification of a Novel AmpC Enzyme (CMY-8) in Southern Taiwan

Jing-Jou Yan,1 Shiou-Mei Wu,2 Shu-Huei Tsai,1 Jiunn-Jong Wu,2,* and Ih-Jen Su1

Department of Pathology,1 National Cheng Kung University Medical Center, and Department of Medical Technology,2 National Cheng Kung University Medical College, Tainan, Taiwan

Received 19 October 1999/Returned for modification 26 January 2000/Accepted 29 February 2000

Twenty (8.5%) of 234 nonrepetitive clinical isolates of Klebsiella pneumoniae from southern Taiwan were found to produce extended-spectrum beta -lactamases (ESBLs): 10 strains produced SHV-12, 4 produced SHV-5, 2 produced a non-TEM non-SHV ESBL with a pI of 8.3, 3 produced a novel AmpC beta -lactamase designated CMY-8 with a pI of 8.25, and 1 produced SHV-12 and an unidentified AmpC enzyme with a pI of 8.2. The CMY-8 enzyme confers a resistance phenotype similar to CMY-1 and MOX-1, and sequence comparisons showed high homologies (>95%) of nucleotide and amino acid sequences among these three enzymes. Plasmid and pulse-field gel electrophoresis analyses revealed that all isolates harboring an SHV-derived ESBL were genetically unrelated, indicating that dissemination of resistance plasmids is responsible for the spread of SHV ESBLs among K. pneumoniae in this area. All three isolates carrying CMY-8 had identical genotypic patterns, suggesting the presence of an epidemic strain.

* Corresponding author. Mailing address: Department of Medical Technology, National Cheng Kung University Medical College, No. 1 University Rd., Tainan, Taiwan 70101, Taiwan. Phone: 886-6-2353535, ext. 5775. Fax: 886-6-2363956. E-mail: jjwu{at}mail.ncku.edu.tw.

Antimicrobial Agents and Chemotherapy, June 2000, p. 1438-1442, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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