This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Girlich, D.
Right arrow Articles by Nordmann, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Girlich, D.
Right arrow Articles by Nordmann, P.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, June 2000, p. 1470-1478, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Biochemical-Genetic Characterization and Regulation of Expression of an ACC-1-Like Chromosome-Borne Cephalosporinase from Hafnia alvei

Delphine Girlich, Thierry Naas, Samuel Bellais, Laurent Poirel, Amal Karim, and Patrice Nordmann*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre cedex, France

Received 14 September 1999/Returned for modification 24 January 2000/Accepted 17 March 2000

A naturally occurring AmpC beta -lactamase (cephalosporinase) gene was cloned from the Hafnia alvei 1 clinical isolate and expressed in Escherichia coli. The deduced AmpC beta -lactamase (ACC-2) had a pI of 8 and a relative molecular mass of 37 kDa and showed 50 and 47% amino acid identity with the chromosome-encoded AmpCs from Serratia marcescens and Providentia stuartii, respectively. It had 94% amino acid identity with the recently described plasmid-borne cephalosporinase ACC-1 from Klebsiella pneumoniae, suggesting the chromosomal origin of ACC-1. The hydrolysis constants (kcat and Km) showed that ACC-2 was a peculiar cephalosporinase, since it significantly hydrolyzed cefpirome. Once its gene was cloned and expressed in E. coli (pDEL-1), ACC-2 conferred resistance to ceftazidime and cefotaxime but also an uncommon reduced susceptibility to cefpirome. A divergently transcribed ampR gene with an overlapping promoter compared with ampC (blaACC-2) was identified in H. alvei 1, encoding an AmpR protein that shared 64% amino acid identity with the closest AmpR protein from P. stuartii. beta -Lactamase induction experiments showed that the ampC gene was repressed in the absence of ampR and was activated when cefoxitin or imipenem was added as an inducer. From H. alvei 1 cultures that expressed an inducible-cephalosporinase phenotype, several ceftazidime- and cefpirome-cross-resistant H. alvei 1 mutants were obtained upon selection on cefpirome- or ceftazidime-containing plates, and H. alvei 1 DER, a ceftazidime-resistant mutant, stably overproduced cephalosporinase. Transformation of H. alvei 1 DER or E. coli JRG582 (ampDE mutant) harboring ampC and ampR from H. alvei 1 with a recombinant plasmid containing ampD from E. coli resulted in a decrease in the MIC of beta -lactam and recovery of an inducible phenotype for H. alvei 1 DER. Thus, AmpR and AmpD proteins may regulate biosynthesis of the H. alvei cephalosporinase similarly to other enterobacterial cephalosporinases.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78, rue du Général Leclerc, 94275 Le Kremlin-Bicêtre cedex, France. Phone: 33-1-45 21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, June 2000, p. 1470-1478, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Savini, V., Catavitello, C., Talia, M., Balbinot, A., Febbo, F., Pompilio, A., Di Bonaventura, G., Piccolomini, R., D'Antonio, D. (2009). Isolation of colistin-resistant Hafnia alvei. J Med Microbiol 58: 278-280 [Full Text]  
  • Jacoby, G. A. (2009). AmpC {beta}-Lactamases. Clin. Microbiol. Rev. 22: 161-182 [Abstract] [Full Text]  
  • Papagiannitsis, C. C., Tzouvelekis, L. S., Tzelepi, E., Miriagou, V. (2007). Plasmid-Encoded ACC-4, an Extended-Spectrum Cephalosporinase Variant from Escherichia coli. Antimicrob. Agents Chemother. 51: 3763-3767 [Abstract] [Full Text]  
  • Partridge, S. R., Arlet, G. (2007). Genetic Environment of ISEcp1 and blaACC-1. Antimicrob. Agents Chemother. 51: 2658-2659 [Full Text]  
  • Stone, N. D., O'Hara, C. M., Williams, P. P., McGowan, J. E. Jr., Tenover, F. C. (2007). Comparison of Disk Diffusion, VITEK 2, and Broth Microdilution Antimicrobial Susceptibility Test Results for Unusual Species of Enterobacteriaceae. J. Clin. Microbiol. 45: 340-346 [Abstract] [Full Text]  
  • Ruppe, E., Bidet, P., Verdet, C., Arlet, G., Bingen, E. (2006). First Detection of the Ambler Class C 1 AmpC {beta}-Lactamase in Citrobacter freundii by a New, Simple Double-Disk Synergy Test. J. Clin. Microbiol. 44: 4204-4207 [Abstract] [Full Text]  
  • Girlich, D., Naas, T., Nordmann, P. (2006). Regulation of class D beta-lactamase gene expression in Ralstonia pickettii.. Microbiology 152: 2661-2672 [Abstract] [Full Text]  
  • Janda, J. M., Abbott, S. L. (2006). The Genus Hafnia: from Soup to Nuts. Clin. Microbiol. Rev. 19: 12-28 [Abstract] [Full Text]  
  • Bidet, P., Burghoffer, B., Gautier, V., Brahimi, N., Mariani-Kurkdjian, P., El-Ghoneimi, A., Bingen, E., Arlet, G. (2005). In Vivo Transfer of Plasmid-Encoded ACC-1 AmpC from Klebsiella pneumoniae to Escherichia coli in an Infant and Selection of Impermeability to Imipenem in K. pneumoniae. Antimicrob. Agents Chemother. 49: 3562-3565 [Abstract] [Full Text]  
  • Lau, S. K. P., Ho, P.-l., Li, M. W. S., Tsoi, H.-w., Yung, R. W. H., Woo, P. C. Y., Yuen, K.-y. (2005). Cloning and Characterization of a Chromosomal Class C {beta}-Lactamase and Its Regulatory Gene in Laribacter hongkongensis. Antimicrob. Agents Chemother. 49: 1957-1964 [Abstract] [Full Text]  
  • Poirel, L., Lartigue, M.-F., Decousser, J.-W., Nordmann, P. (2005). ISEcp1B-Mediated Transposition of blaCTX-M in Escherichia coli. Antimicrob. Agents Chemother. 49: 447-450 [Abstract] [Full Text]  
  • Reisbig, M. D., Hanson, N. D. (2004). Promoter Sequences Necessary for High-Level Expression of the Plasmid-Associated ampC {beta}-Lactamase Gene blaMIR-1. Antimicrob. Agents Chemother. 48: 4177-4182 [Abstract] [Full Text]  
  • Naas, T., Aubert, D., Vimont, S., Nordmann, P. (2004). Identification of a chromosome-borne class C {beta}-lactamase from Erwinia rhapontici. J Antimicrob Chemother 54: 932-935 [Abstract] [Full Text]  
  • Reisbig, M. D., Hossain, A., Hanson, N. D. (2003). Factors influencing gene expression and resistance for Gram-negative organisms expressing plasmid-encoded ampC genes of Enterobacter origin. J Antimicrob Chemother 51: 1141-1151 [Abstract] [Full Text]  
  • Decre, D., Verdet, C., Raskine, L., Blanchard, H., Burghoffer, B., Philippon, A., Sanson-Le-Pors, M. J., Petit, J. C., Arlet, G. (2002). Characterization of CMY-type {beta}-lactamases in clinical strains of Proteus mirabilis and Klebsiella pneumoniae isolated in four hospitals in the Paris area. J Antimicrob Chemother 50: 681-688 [Abstract] [Full Text]  
  • Doi, Y., Shibata, N., Shibayama, K., Kamachi, K., Kurokawa, H., Yokoyama, K., Yagi, T., Arakawa, Y. (2002). Characterization of a Novel Plasmid-Mediated Cephalosporinase (CMY-9) and Its Genetic Environment in an Escherichia coli Clinical Isolate. Antimicrob. Agents Chemother. 46: 2427-2434 [Abstract] [Full Text]  
  • Perez-Perez, F. J., Hanson, N. D. (2002). Detection of Plasmid-Mediated AmpC {beta}-Lactamase Genes in Clinical Isolates by Using Multiplex PCR. J. Clin. Microbiol. 40: 2153-2162 [Abstract] [Full Text]  
  • Fortineau, N., Poirel, L., Nordmann, P. (2001). Plasmid-mediated and inducible cephalosporinase DHA-2 from Klebsiella pneumoniae. J Antimicrob Chemother 47: 207-210 [Abstract] [Full Text]  
  • Girlich, D., Naas, T., Bellais, S., Poirel, L., Karim, A., Nordmann, P. (2000). Heterogeneity of AmpC Cephalosporinases of Hafnia alvei Clinical Isolates Expressing Inducible or Constitutive Ceftazidime Resistance Phenotypes. Antimicrob. Agents Chemother. 44: 3220-3223 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»