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Antimicrobial Agents and Chemotherapy, June 2000, p. 1518-1523, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Morphological Changes and Lysis Induced by beta -Lactams Associated with the Characteristic Profiles of Affinities of Penicillin-Binding Proteins in Actinobacillus pleuropneumoniae

Takashi Inui,* Toshio Endo, and Tadahiro Matsushita

Discovery Research Laboratory, Tanabe Seiyaku Co., Ltd., 2-2-50, Kawagishi, Toda-shi, Saitama 335-8505, Japan

Received 6 July 1999/Returned for modification 3 November 1999/Accepted 10 March 2000

Actinobacillus pleuropneumoniae, which was formerly classified in the genus Haemophilus, is a pathogen causing swine pleuropneumonia. We found that aspoxicillin showed strong activity and that meropenem had better lytic activity against this pathogen. In the present study, we for the first time identified penicillin-binding proteins (PBPs) of A. pleuropneumoniae in order to elucidate the relationship between the antibacterial and lytic activities of beta -lactam antibiotics and affinities of the PBPs. The competitive assay using 3H-labeled benzylpenicillin revealed seven PBPs in A. pleuropneumoniae; they were determined to be PBPs 1a, 1b, 2, 3, 4, 5, and 6, and the molecular masses of these PBPs were estimated to be 92, 80, 76, 72, 50, 44, and 30 kDa, respectively, by comparison with those of Haemophilus influenzae. Our detailed analysis of the affinities of the PBPs of A. pleuropneumoniae and of the bacterial lysis kinetics for several beta -lactam antibiotics revealed that the strong antibacterial activity of aspoxicillin against this strain could be related to the higher affinity of PBP 3 and that preferential inactivation of PBP 1b could cause rapid lysis.

* Corresponding author. Mailing address: Discovery Research Laboratory, Tanabe Seiyaku Co., Ltd., 2-2-50, Kawagishi, Toda-shi, Saitama 335-8505, Japan. Phone: 81-48-433-8071. Fax: 81-48-433-8161. E-mail: tksh{at}tanabe.co.jp.

Antimicrobial Agents and Chemotherapy, June 2000, p. 1518-1523, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Morikawa, Y., Kitazato, M., Mitsuyama, J., Mizunaga, S., Minami, S., Watanabe, Y. (2004). In Vitro Activities of Piperacillin against {beta}-Lactamase-Negative Ampicillin-Resistant Haemophilus influenzae. Antimicrob. Agents Chemother. 48: 1229-1234 [Abstract] [Full Text]  


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