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Antimicrobial Agents and Chemotherapy, June 2000, p. 1694-1696, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

In Vitro Susceptibility Tests for Cationic Peptides: Comparison of Broth Microdilution Methods for Bacteria That Grow Aerobically

A. Giacometti,* O. Cirioni, F. Barchiesi, M. S. Del Prete, M. Fortuna, F. Caselli, and G. Scalise

Institute of Infectious Diseases and Public Health, University of Ancona, Italy

Received 13 September 1999/Returned for modification 7 January 2000/Accepted 26 February 2000

The in vitro susceptibilities of 90 clinical isolates of gram-positive and gram-negative aerobic bacteria to six cationic peptides, buforin II, cecropin P1, indolicidin, magainin II, nisin, and ranalexin, were evaluated by two broth microdilution methods. The first method was performed according to the procedures outlined by the National Committee for Clinical Laboratory Standards for bacteria that grow aerobically, while the second was performed according to the procedures recently proposed by the R. E. W. Hancock laboratory for testing antimicrobial peptides. Overall, the first method produced MICs two- and fourfold higher than the second method.

* Corresponding author. Mailing address: Clinica delle Malattie Infettive, c/o Azienda Ospedaliera Umberto I, Piazza Cappelli, 1, 60121 Ancona, Italy. Phone: 39 71 5963467. Fax: 39 71 5963468. E-mail: cmalinf{at}popcsi.unian.it.

Antimicrobial Agents and Chemotherapy, June 2000, p. 1694-1696, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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