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Antimicrobial Agents and Chemotherapy, June 2000, p. 1713-1715, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Mutations at Four Distinct Regions of the rpoB Gene Can Reduce the Susceptibility of Helicobacter pylori to Rifamycins

Markus Heep,1,* Stefan Odenbreit,2 Daniela Beck,1 Jochen Decker,1 Elke Prohaska,1 Ulrich Rieger,1 and Norbert Lehn1

Institut für Medizinische Mikrobiologie, Universität Regensburg, Regensburg,1 and Max-von-Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Universität München, Munich,2 Germany

Received 27 August 1999/Returned for modification 21 December 1999/Accepted 6 March 2000

The transformation of a panel of rpoB mutations (codons 525 to 586) from rifampin-resistant donor strains into Helicobacter pylori 2802A confirmed the MICs associated with the respective mutations. RpoB V149X random mutations were generated and induced different levels of resistance, depending on the replacement amino acid. Mutagenesis of the rpoB gene at codon position 701 (R701H) induced low-level resistance.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie, Uniklinik Regensburg, D-93053, Regensburg, Germany. Phone: 49 941 944 6414. Fax: 49 941 944 6402. E-mail: Markus.Heep{at}klinik.uni-regensburg.de.

Antimicrobial Agents and Chemotherapy, June 2000, p. 1713-1715, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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