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Antimicrobial Agents and Chemotherapy, June 2000, p. 1734-1736, Vol. 44, No. 6
Mycotic Diseases Branch, Division of
Bacterial and Mycotic Diseases, National Center for Infectious
Diseases, Centers for Disease Control and Prevention, Atlanta,
Georgia 30333,1 and Department of
Pathology, University of Texas Medical Branch, Galveston, Texas
77555-06092
Received 23 September 1999/Returned for modification 7 March
2000/Accepted 21 March 2000
The in vitro activity of voriconazole was compared to those of
itraconazole and amphotericin B against the mold forms of 304 isolates
of three dimorphic fungi, Blastomyces dermatitidis,
Coccidioides immitis, and Histoplasma
capsulatum. MICs were determined by a broth microdilution
adaptation of the National Committee for Clinical Laboratory Standards
M27-A procedure. RPMI 1640 medium was used for tests with voriconazole
and itraconazole, whereas Antibiotic Medium 3 with 2% glucose was used
for amphotericin B. Minimum fungicidal concentrations (MFCs) were also
determined. Amphotericin B was active against all three dimorphic
fungi, with MICs at which 90% of the isolates tested are inhibited
(MIC90s) of 0.5 to 1 µg/ml. Itraconazole had
MIC90s of 0.06 µg/ml for H. capsulatum, 0.125 µg/ml for B. dermatitidis, and 1 µg/ml for C. immitis. The MIC90s of voriconazole were 0.25 µg/ml
for all three fungi. Amphotericin B was fungicidal for B. dermatitidis and H. capsulatum with MFCs at which
90% of strains tested are killed (MFC90s) of 0.5 and 2 µg/ml, respectively. It was less active against C. immitis, with MFCs ranging from 0.5 to >16 µg/ml. Voriconazole
and itraconazole were lethal for most isolates of B. dermatitidis, with MFC50s and MFC90s of
0.125 and 4 µg/ml, respectively. Both azoles were fungicidal for some
isolates of H. capsulatum, with MFC50s of 2 and
8 µg/ml for itraconazole and voriconazole, respectively; neither had
a lethal effect upon C. immitis. Our results suggest that
voriconazole possesses promising activity against these important human pathogens.
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
In Vitro Activities of Voriconazole, Itraconazole,
and Amphotericin B against Blastomyces dermatitidis,
Coccidioides immitis, and Histoplasma
capsulatum
*
Corresponding author. Mailing address: Mycotic Diseases
Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd., N.E., Mailstop C-09, Atlanta, GA 30333. Phone: (404) 639-3053. Fax:
(404) 639-2780. E-mail: dsw8{at}cdc.gov.
Antimicrobial Agents and Chemotherapy, June 2000, p. 1734-1736, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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