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Antimicrobial Agents and Chemotherapy, August 2000, p. 2034-2038, Vol. 44, No. 8
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

beta -Lactamases in Shigella flexneri Isolates from Hong Kong and Shanghai and a Novel OXA-1-Like beta -Lactamase, OXA-30

L. K. Siu,1,dagger J. Y. C. Lo,2 K. Y. Yuen,1 P. Y. Chau,1 M. H. Ng,1 and P. L. Ho1,*

Department of Microbiology, The University of Hong Kong,1 and Department of Health,2 Hong Kong, China

Received 8 October 1999/Returned for modification 5 April 2000/Accepted 11 May 2000

Ninety-one ampicillin-resistant Shigella flexneri strains from Hong Kong and Shanghai were studied for production of beta -lactamases. TEM-1-like and OXA-1-like enzymes were identified in 21 and 79% of the strains, respectively, by isoelectric focusing (IEF). No difference in the pattern of beta -lactamase production was found between strains from Hong Kong and Shanghai. Four ribotypes were detected. Over 88% of OXA-producing strains had the same ribotype. All TEM-1-like strains harbored a plasmid which hybridized positively with the blaTEM probe. Total DNA from OXA-1-like strains failed to hybridize or only hybridized weakly with an OXA probe. The OXA resistance was not transferable. OXA-1-like enzymes exhibited substrate and inhibition profiles similar to that of OXA-1 and were shown to have a pI of 7.3 by further IEF using a narrow-range ampholine gel. The gene encoding the OXA-1-like enzyme from one isolate (CH-07) was cloned, sequenced, and found to differ from blaOXA-1 at codon 131 (AGAright-arrowGGA; Arg to Gly), resulting in the novel designation OXA-30. The predominance of OXA-type enzymes in ampicillin-resistant S. flexneri suggests host preference for specific beta -lactamases.

* Corresponding author. Mailing address: Department of Microbiology, The University of Hong Kong, Pokfulam Rd., Hong Kong, China. Phone: (852) 28554892. Fax: (852) 28551241. E-mail: plho{at}hkucc.hku.hk.

dagger Present address: Clinical Research Division, National Health Research Institute, Taipei, Taiwan, Republic of China.

Antimicrobial Agents and Chemotherapy, August 2000, p. 2034-2038, Vol. 44, No. 8
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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