AAC
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Siu, L. K.
Right arrow Articles by Ho, P. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Siu, L. K.
Right arrow Articles by Ho, P. L.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, August 2000, p. 2034-2038, Vol. 44, No. 8
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

beta -Lactamases in Shigella flexneri Isolates from Hong Kong and Shanghai and a Novel OXA-1-Like beta -Lactamase, OXA-30

L. K. Siu,1,dagger J. Y. C. Lo,2 K. Y. Yuen,1 P. Y. Chau,1 M. H. Ng,1 and P. L. Ho1,*

Department of Microbiology, The University of Hong Kong,1 and Department of Health,2 Hong Kong, China

Received 8 October 1999/Returned for modification 5 April 2000/Accepted 11 May 2000

Ninety-one ampicillin-resistant Shigella flexneri strains from Hong Kong and Shanghai were studied for production of beta -lactamases. TEM-1-like and OXA-1-like enzymes were identified in 21 and 79% of the strains, respectively, by isoelectric focusing (IEF). No difference in the pattern of beta -lactamase production was found between strains from Hong Kong and Shanghai. Four ribotypes were detected. Over 88% of OXA-producing strains had the same ribotype. All TEM-1-like strains harbored a plasmid which hybridized positively with the blaTEM probe. Total DNA from OXA-1-like strains failed to hybridize or only hybridized weakly with an OXA probe. The OXA resistance was not transferable. OXA-1-like enzymes exhibited substrate and inhibition profiles similar to that of OXA-1 and were shown to have a pI of 7.3 by further IEF using a narrow-range ampholine gel. The gene encoding the OXA-1-like enzyme from one isolate (CH-07) was cloned, sequenced, and found to differ from blaOXA-1 at codon 131 (AGAright-arrowGGA; Arg to Gly), resulting in the novel designation OXA-30. The predominance of OXA-type enzymes in ampicillin-resistant S. flexneri suggests host preference for specific beta -lactamases.

* Corresponding author. Mailing address: Department of Microbiology, The University of Hong Kong, Pokfulam Rd., Hong Kong, China. Phone: (852) 28554892. Fax: (852) 28551241. E-mail: plho{at}hkucc.hku.hk.

dagger Present address: Clinical Research Division, National Health Research Institute, Taipei, Taiwan, Republic of China.

Antimicrobial Agents and Chemotherapy, August 2000, p. 2034-2038, Vol. 44, No. 8
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Clin. Vaccine Immunol. Clin. Microbiol. Rev.
J. Clin. Microbiol. ALL ASM JOURNALS

Copyright © 2000 by the American Society for Microbiology. All rights reserved.